The aim of studying and bibliography

The main aim of study: Introduction to the basic knowledge and terminology in the field of general toxicology. Acquainting with mechanisms of action of toxic substances and foundations of their characteristics.

The general information about the module: The module is introduced in the 5th semester of the first degree/ undergraduate course as a mandatory module for the speciality. It is accomplished with 30 hours of lectures and 15 hours of laboratory work.

Teaching materials: http://www.nrl.uvm.sk/prz/ - stona internetowa dla studentów PRz Katedry Biochemii i Biotechnologii

Bibliography required to complete the module

Bibliography used during lectures

1	Piotrowski J.K.	Podstawy toksykologii	Wydawnictwo Naukowo-Techniczne, Warszawa .	2006
2	Seńczuk W. (red.)	Toksykologia współczesna	PZWL.	2005
3	Manahan S.E.	Toksykologia środowiska. Aspekty chemiczne i biochemiczne.	PWN, Warszawa.	2006
4	Duffus JH., Worth HG.	Fundamental toxicology	RSCP Publishing.	2006

Bibliography used during classes/laboratories/others

1

J. Legáth

Ćwiczenia z toksykologii

http://www.nrl.uvlf.sk/prz/cwt.html.

2013

Basic requirements in category knowledge/skills/social competences

Formal requirements: Positively finished courses: Cell Biology, General and Inorganic Chemistry, Organic Chemistry and Biochemistry

Basic requirements in category knowledge: Basic knowledge of inorganic and organic chemistry and biochemistry.

Basic requirements in category skills: Self-education and searching for information skills.

Basic requirements in category social competences: Individual and team work skills.

Module outcomes

MEK	The student who completed the module	Types of classes / teaching methods leading to achieving a given outcome of teaching	Methods of verifying every mentioned outcome of teaching	Relationships with KEK	Relationships with PRK
01	Knows the basic issues of impact of chemical substances on natural environment.	lecture	written test
02	Knows basic techniques and methods for poisons determination, their analysis and basic principles of poisoning diagnostics	lecture	written test	K_W14+++ K_U19++	P6S_UO P6S_UW P6S_WG
03	Properly uses toxicological nomenclature.	lecture, laboratory work	written test	K_U03+++	P6S_UK
04	Can properly handle with samples for analysis and determine the content of toxic substances in biological sample.	laboratory work	written test
05	Realizes the importance of engineering, its effects and impact on natural environment and the responsibilities arising from made decisions	lecture	written test	K_K02+++	P6S_KO P6S_KR
06	Is able to correctly identify and resolve dilemmas associated with the pursuit of profession.	lecture	written test	K_K05+++	P6S_KK P6S_KR

Attention: Depending on the epidemic situation, verification of the achieved learning outcomes specified in the study program, in particular credits and examinations at the end of specific classes, can be implemented remotely (real-time meetings).

The syllabus of the module

Sem.	TK	The content	realized in	MEK
6	TK01	Introduction on the toxicology, definition of poison, intoxication, intoxication types, toxicity of chemical compounds, accumulation, persistence, way of introduction of poisons in the organisms.	W01	MEK01 MEK03
6	TK02	Factors which influence of toxicity of poisons, synergisms and antagonisms.	W02	MEK01 MEK03
6	TK03	Biotransformation of poisons in the organisms and degradation process of the poisons in the environment, elimination of poisons from organisms (pathway and biochemical mechanisms of elimination), etiology of intoxication, definition of abbreviation which will be used in the toxicology.	W03	MEK01 MEK03
6	TK04	Prevention of the intoxication and basic therapy of intoxication REACH process – legislative in the European Union.	W04	MEK05 MEK06
6	TK05	Risk assessment, definition of RA, identification of harmful substance, dose – response, exposition, risk characteristic, calculation of ADI (or RfD) and LD50, definition of abbreviation NOEL, NOAEL, NOEC, NOAEC, SF, UF, MF, ADI	W05	MEK01 MEK03
6	TK06	Practical presentation of risk assessment of use of herbicide in the aquatic environment.	W06	MEK01 MEK05
6	TK07	Developmental toxicology, toxicology versus spermatogenesis, oogenesis and fertilization. Evaluation of toxic compounds on the embryo and developmental organism after birth to adulthood.	W07	MEK01
6	TK08	Toxicology of choice inorganic compounds (CO, CN-, NO2-, NH3, H2S, Cl2, PH3 …). Toxicology of acids and hydroxide.	W08	MEK01
6	TK09	Toxicology of selected organic compounds.	W09	MEK01
6	TK10	Toxicology of selected heavy metals (Pb, Cd, Hg, Cu, As, Ba, Mg ....)	W10	MEK01
6	TK11	Toxicology of pesticides – divide of pesticides according to use in the agricultural practice, toxicology of selected pesticides according to chemical groups	W11	MEK01
6	TK12	Intoxications of selected drugs	W12	MEK02 MEK06
6	TK13	Mycotoxins - characterization, toxicity, risk, divide by effect of the living organism	W13	MEK01 MEK02
6	TK14	Poison plants – chemical compounds of toxic plants, divide toxic plants by effect of the living organism (by effect on the bodily organs)	W14	MEK01
6	TK15	Poisonous animals – chemical compounds of animal toxins, representative animal species.	W15	MEK01
6	TK16	General information about toxicology, diagnose of intoxication, sampling, packing and sending for chemical toxicology analysis	L01	MEK04
6	TK17	Determination of noxa in biological material without samples adjustment	L02	MEK04
6	TK18	Determination of toxicologically important chemical compounds separable by water steam distillation	L03	MEK04
6	TK19	Determination of warfarine (kumarine) in the biological material	L04	MEK04
6	TK20	Determination of alkaloids in biological material by TLC method	L05	MEK04
6	TK21	Determination of drugs in the biological material by TLC method (salinomycín, monenzin, paracetamol)	L06	MEK04
6	TK22	Determination of herbicides MCPA and DNOK in the biological material	L07	MEK04

The student's effort

The type of classes	The work before classes	The participation in classes	The work after classes
Lecture (sem. 6)		contact hours: 30.00 hours/sem.	complementing/reading through notes: 5.00 hours/sem.
Laboratory (sem. 6)	The preparation for a Laboratory: 4.00 hours/sem. The preparation for a test: 8.00 hours/sem.	contact hours: 15.00 hours/sem.
Advice (sem. 6)
Credit (sem. 6)	The preparation for a Credit: 12.00 hours/sem.	The written credit: 1.00 hours/sem.

The way of giving the component module grades and the final grade

The type of classes	The way of giving the final grade
Lecture
Laboratory
The final grade	The final note (OK) is calculated based on the sum of points from tests (OW+OL) 51-60p. - 3.0; 61-70p. - 3.5; 71-80p. - 4.0; 81-90p. - 4.5; 91-100p. - 5.0

Sample problems

Required during the exam/when receiving the credit
(-)

Realized during classes/laboratories/projects
(-)

Others
(-)

Can a student use any teaching aids during the exam/when receiving the credit : no

The contents of the module are associated with the research profile: yes

1	A. Bocian; J. Buczkowicz; K. Hus; J. Legath; T. Litschka-Koen; V. Petrilla; M. Petrillova; M. Pietrowska	Venom diversity in Naja mossambica: Insights from proteomic and immunochemical analyses reveal intraspecific differences	2024
2	B. Bakera; M. Rakoczy-Trojanowska; M. Szeliga; M. Święcicka; M. Tyrka	Identification of candidate genes responsible for chasmogamy in wheat	2023
3	P. Bednarek; A. Dorczyk; T. Drzazga; D. Jasińska; P. Krajewski; B. Ługowska; R. Martofel; P. Matysik; M. Niewińska; D. Ratajczak; K. Rączka; T. Sikora; D. Tyrka; M. Tyrka; E. Witkowski; U. Woźna-Pawlak	Genome-wide association mapping in elite winter wheat breeding for yield improvement	2023
4	A. Bocian; J. Gikunju; K. Hus; J. Kimotho ; J. Legath; E. Manson; K. Mutinda; V. Petrilla	Development of an Inhibition Enzyme-Linked Immunosorbent Assay (ELISA) Prototype for Detecting Cytotoxic Three-Finger Toxins (3FTxs) in African Spitting Cobra Venoms	2022
5	A. Bocian; K. Hus; J. Kimani; J. Kimotho ; M. Kyama; J. Legath; E. Manson; V. Petrilla	Development and Characterization of Anti-Naja ashei Three-Finger Toxins (3FTxs)-Specific Monoclonal Antibodies and Evaluation of Their In Vitro Inhibition Activity	2022
6	M. Dyda; G. Gołębiowska; M. Rapacz; M. Szechyńska-Hebda; M. Tyrka; I. Wąsek; M. Wędzony	Quantitative trait loci and candidate genes associated with freezing tolerance of winter triticale (× Triticosecale Wittmack)	2022
7	M. Dyda; G. Gołębiowska; M. Rapacz; M. Tyrka; M. Wędzony	Genetic mapping of adult-plant resistance genes to powdery mildew in triticale	2022
8	M. Dyda; G. Gołębiowska; M. Rapacz; M. Tyrka; M. Wędzony	Mapping of QTL and candidate genes associated with powdery mildew resistance in triticale (× Triticosecale Wittm.)	2022
9	P. Krajewski; R. Marcinkowski; R. Martofel; P. Matysik; M. Mokrzycka; M. Rakoczy-Trojanowska; M. Rokicki; S. Stojałowski; M. Tyrka; U. Woźna-Pawlak; B. Żmijewska	Genome-Wide Association Analysis for Hybrid Breeding in Wheat	2022
10	A. Barbasz; A. Bocian; A. Czyżowska; B. Dyba; K. Hus; J. Legath; V. Petrilla; M. Petrillova; E. Rudolphi-Szydło	Effects of 3FTx Protein Fraction from Naja ashei Venom on the Model and Native Membranes: Recognition and Implications for the Mechanisms of Toxicity	2021
11	A. Pietrusińska; M. Tyrka	Linkage of Lr55 wheat leaf rust resistance gene with microsatellite and DArT-based markers	2021
12	B. Bakera; P. Krajewski; M. Mokrzycka; M. Rakoczy-Trojanowska; M. Szeliga; M. Święcicka; M. Tyrka	Identification of Rf Genes in Hexaploid Wheat (Triticumaestivum L.) by RNA-Seq and Paralog Analyses	2021
13	B. Bakera; P. Krajewski; P. Matysik; M. Mokrzycka; M. Rakoczy-Trojanowska; M. Rokicki; S. Stojałowski; M. Szeliga; D. Tyrka; M. Tyrka	Evaluation of genetic structure in European wheat cultivars and advanced breeding lines using high-density genotyping-by-sequencing approach	2021
14	J. Buczkowicz; T. Drzazga; B. Ługowska; P. Matysik; K. Rubrycki; M. Semik; D. Tyrka; M. Tyrka; E. Witkowski	Identyfikacja efektywnych genów odporności na wybrane choroby wirusowe i grzybowe pszenicy zwyczajnej	2021
15	J. Buczkowicz; T. Drzazga; G. Fic; M. Jaromin; P. Krajewski; P. Matysik; R. Mazur; P. Milczarski; T. Sikora; M. Szeliga; D. Tyrka; M. Tyrka; E. Witkowski	Selekcja genomowa pszenicy ozimej	2021
16	A. Bocian; J. Buczkowicz; E. Ciszkowicz; K. Hus; K. Lecka-Szlachta; J. Legath; L. Legath; V. Petrilla; M. Petrillova; M. Pietrowska	Antimicrobial Activity of Protein Fraction from Naja ashei Venom Against Staphylococcus epidermidis	2020
17	A. Bocian; J. Buczkowicz; K. Hus; M. Jaromin; J. Legath; D. Łysiak; V. Petrilla; M. Petrillova; S. Sławek	Comparison of Methods for Measuring Protein Concentration in Venom Samples	2020
18	A. Bocian; K. Hus; E. Kuna; J. Legath; A. Lewińska; V. Petrilla; M. Petrillova; M. Wnuk	Evaluation of Antifungal Activity of Naja pallida and Naja mossambica Venoms against Three Candida Species	2020
19	A. Bocian; K. Hus; J. Legath; Ł. Marczak; V. Petrilla; M. Petrillova	Different research approaches in unraveling the venom proteome of Naja ashei	2020
20	E. Ciszkowicz; E. Kaznowska; P. Porzycki; M. Semik; M. Tyrka	MiR-93/miR-375: Diagnostic Potential, Aggressiveness Correlation and Common Target Genes in Prostate Cancer	2020
21	G. Czajowski; M. Karbarz; M. Pojmaj; A. Strzembicka; D. Tyrka; M. Tyrka; A. Wardyńska; M. Wędzony	Quantitative trait loci mapping of adult-plant resistance to powdery mildew in triticale	2020
22	J. Ciura; M. Szeliga; M. Tyrka	Representational Difference Analysis of Transcripts Involved in Jervine Biosynthesis	2020
23	A. Bocian; J. Buczkowicz; K. Hus; M. Jaromin; J. Legath	An effective method of isolating honey proteins	2019
24	J. Ciura; M. Grzesik; M. Szeliga; M. Tyrka	Identification of candidate genes involved in steroidal alkaloids biosynthesis in organ-specific transcriptomes of Veratrum nigrum L.	2019
25	J. Legath; D. Marcincakova; M. Miłek	Polyphenols Content, Antioxidant Activity, and Cytotoxicity Assessment of Taraxacum officinale Extracts Prepared through the Micelle-Mediated Extraction Method	2019
26	M. Dyda; M. Szechyńska-Hebda; M. Tyrka; I. Wąsek; M. Wędzony	Local and systemic regulation of PSII efficiency in triticale infected by the hemibiotrophic pathogen Microdochium nivale	2019
27	M. Dziurka; K. Hura; T. Hura; A. Ostrowska; M. Tyrka	Participation of Wheat and Rye Genome in Drought Induced Senescence in Winter Triticale (X Triticosecale Wittm.)	2019
28	T. Csank; M. Falis; M. Fedorova; K. Hus; J. Legath; S. Marcincak; D. Marcincakova; D. Mudronova; P. Schusterova	Impact of Zinc Sulfate Exposition on Viability, Proliferation and Cell Cycle Distribution of Epithelial Kidney Cells	2019
29	Z. Banaszak; A. Fiust; Z. Nita; W. Orłowska-Job; M. Pojmaj; M. Rapacz; M. Tyrka; M. Wójcik-Jagła	Sposób selekcji mrozoodpornych genotypów jęczmienia ozimego	2019