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Immunological techniques in biotechnology

Some basic information about the module

Cycle of education: 2022/2023

The name of the faculty organization unit: The faculty Chemistry

The name of the field of study: Biotechnology

The area of study: technical sciences

The profile of studing:

The level of study: first degree study

Type of study: full time

discipline specialities : Applied biochemistry, Purification and analysis of biotechnological products

The degree after graduating from university: Bachelor of Science (BSc)

The name of the module department : Department of Biochemistry and Bioinformatics

The code of the module: 254

The module status: mandatory for teaching programme

The position in the studies teaching programme: sem: 5 / W30 L30 / 4 ECTS / Z

The language of the lecture: Polish

The name of the coordinator: Aleksandra Bocian, DSc, PhD

semester 5: Ewa Ciszkowicz, PhD

The aim of studying and bibliography

The main aim of study: Learning the immunological techniques in biological studies with particular emphasis on biotechnology

The general information about the module: The module is introduced in the 5th semester of the first degree studies. It is accomplished with 30 hours of lecture and 30 hours of laboratory work. It ends with an examination-based assessment.

Bibliography required to complete the module
Bibliography used during lectures
1 Ptak Włodzimierz, Ptak Maria, Szczepanik Marian Podstawy immunologii PZWL. 2010
2 P.M. Lydyard, A. Whelan, M.W. Fanger Immunologia. Krótkie wykłady Wydawnictwo Naukowe PWN. 2009
3 Male D. Immunologia Wroclaw. 2008
4 Golab J., Jakobsiak M., Lasek W. Immunologia. Nowe wydanie Warszawa. 2017
Bibliography used during classes/laboratories/others
1 Teresa Kaminska Cwiczenia z immunologii Lublin. Wydawnictwo Uniwersytetu Marii Curie-Sklodowskiej. 2013
2 Motyka Kaudefer-Szerszen Cwiczenia z immunologii Lublin. Podreczniki laboratoryjne. 2006
Bibliography to self-study
1 Solnica B., Sztefko K. Medyczne laboratorium diagnostyczne. Metodyka i aparatura Wydawnictwo Lekarskie PZWL. 2015

Basic requirements in category knowledge/skills/social competences

Formal requirements: Valid registration

Basic requirements in category knowledge: Basic knowledge of how living organisms function

Basic requirements in category skills: Self-education skills

Basic requirements in category social competences: Ability to work both alone and in a group

Module outcomes

MEK The student who completed the module Types of classes / teaching methods leading to achieving a given outcome of teaching Methods of verifying every mentioned outcome of teaching Relationships with KEK Relationships with PRK
01 Has knowledge of structure and mechanisms of immune system Lecture Written exam K_W05++
P6S_WG
02 Knows the examples of practical use of immunological mechanisms in biotechnology lecture Written exam K_W09+++
K_W14++
P6S_WG
03 Has the ability to self-study Lecture Written exam K_U06+++
P6S_UU
04 Is able to us the basic immunological laboratory techniques to analyze macromolecules with particular emphasis on their detection with methods based on the antigen-antibody reaction Laboratory work Progress observation K_U09++
K_U15++
K_U17++
P6S_UO
P6S_UW
05 Understands the need of improving their skills and knowledge resulting from the constantly expanding knowledge of the processes and immunological phenomena, and the development of new techniques based on them Lecture Written exam K_K01+++
P6S_KK
P6S_KR
06 Knows how to work in a group and individually, can make decisions and carry out the instructions of superiors Laboratory work Progress observation K_K03+++
P6S_KR

Attention: Depending on the epidemic situation, verification of the achieved learning outcomes specified in the study program, in particular credits and examinations at the end of specific classes, can be implemented remotely (real-time meetings).

The syllabus of the module

Sem. TK The content realized in MEK
5 TK01 The structure and functioning of the immune system, specific and non-specific immunity. W1-W6 MEK01 MEK03
5 TK02 Antigens and the mechanisms of their identification. Characteristics of innate and acquired immunological response mechanisms. Mechanism of receptor activation in B and T cells by an antigen: antigen processing and presentation W6-W8 MEK01
5 TK03 In vivo production of monoclonal and polyclonal antibodies. Obtaining monoclonal antibodies using the method of in vivo and in vitro immunization, and the method of genetic engineering W14-W15 MEK05
5 TK04 Methods of the qualitative and quantitative evaluation of detectable macromolecules, using the ELISA method, immunoprecipitation, immunobloting, flow cytometry W9-W13 MEK02 MEK04
5 TK05 Immunological techniques: immunodiffusion, electrophoresis, rocket electrophoresis, Western blot, ELISA L1-L6 MEK04 MEK06

The student's effort

The type of classes The work before classes The participation in classes The work after classes
Lecture (sem. 5) The preparation for a test: 6.00 hours/sem.
contact hours: 30.00 hours/sem.
complementing/reading through notes: 6.00 hours/sem.
Laboratory (sem. 5) The preparation for a Laboratory: 6.00 hours/sem.
The preparation for a test: 8.00 hours/sem.
contact hours: 30.00 hours/sem.
Finishing/Making the report: 6.00 hours/sem.
Advice (sem. 5)
Credit (sem. 5) The preparation for a Credit: 12.00 hours/sem.
The written credit: 2.00 hours/sem.

The way of giving the component module grades and the final grade

The type of classes The way of giving the final grade
Lecture The grade is issued in a manner appropriate to the number of points received during the exam: 50-59% - [3], 60-69% - [3,5], 70-79% - [4], 80-89 - [4,5], 90-100 - [5]
Laboratory The grade is issued on the basis of the average grade of the tests. Test points: 50-59% - [3], 60-69% - [3,5], 70-79% - [4], 80-89 - [4,5, 90-100 - [5]. All reports must be included for a positive final assessment.
The final grade The final grade is the average of the grade of lectures and a final evaluation labs K=0,5wL+0,5wZ (L-llaboratory, Z- final test,, w-coefficient taking into account term, w = 1 for the first term. W = 0.9 for the second term in = 0.8 for the third term). The average may be drawn only if all grades are positive

Sample problems

Required during the exam/when receiving the credit
(-)

Realized during classes/laboratories/projects
(-)

Others
(-)

Can a student use any teaching aids during the exam/when receiving the credit : no

The contents of the module are associated with the research profile: no