The aim of studying and bibliography

The main aim of study: Students are acquainting with techniques of purify biotechnological products.

The general information about the module: The student is getting the knowledge in the scope of mixtures separation and cleaning of a products with membrane, chromatography and other methods. During lectures conducted in the form of presentations, visualizations of mechanisms, construction of membranes, diagrams of discussed processes made in AutoCAD and Autodesk Inventor Professional are presented.

Bibliography required to complete the module

Bibliography used during lectures

1	R. Rautenbach	Procesy membranowe	WNT.	1996
2	W. Szewczyk	Technologia biochemiczna	Oficyna Wydawn. Pol. Warszawska.	2003
3	A. Selecki, R. Gawroński	Podstawy projektowania wybranych procesów rozdzielania mieszanin	WNT.	1992
4	Antos Dorota, Kaczmarski Krzysztof, Piątkowski Wojciech	Chromatografia preparatywna jako proces rozdzielania mieszanin	WNT.	2011

Bibliography used during classes/laboratories/others

1	praca zbiorowa pod red. R Petrusa	Inżynieria Chemiczna Laboratorium	Of. Wyd. PRz.	1998
2	W. Zapała, K. Kaczmarski, I. Poplewska, W. Piątkowski	Wybrane operacje jednostkowe w inżynierii procesowej	Of. Wyd. PRz.	2012

Basic requirements in category knowledge/skills/social competences

Formal requirements: Registration for the given semester.

Basic requirements in category knowledge: Student knows basic methods, techniques, tools and materials applied for solving basic problems connected with the biotechnology.

Basic requirements in category skills: Student has a skill for self-education.

Basic requirements in category social competences: team work

Module outcomes

MEK	The student who completed the module	Types of classes / teaching methods leading to achieving a given outcome of teaching	Methods of verifying every mentioned outcome of teaching	Relationships with KEK	Relationships with PRK
01	Student is getting the knowledge about typpical unite operation proceses leading to isolation from the post-reaction mixture a required product. He knows physico-chemical principels of the separation and preparing the product with following methods: permeation, chromatography, drying and crystallization.	lecture	Colloquium	K_W10++ K_K03+	P6S_KR P6S_WG
02	He is able to separate required species with chromatography, drying and crystallization methods.	laboratory	pass all exercises	K_W10++ K_U17+	P6S_UO P6S_UW P6S_WG

Attention: Depending on the epidemic situation, verification of the achieved learning outcomes specified in the study program, in particular credits and examinations at the end of specific classes, can be implemented remotely (real-time meetings).

The syllabus of the module

Sem.	TK	The content	realized in	MEK
7	TK01	Strategies to recover and purify product. The permeate techniques of the mixtures separation: ultrafiltration, osmosis, reverse osmosis, microfiltration, dialysis, electrodialysis. Mathematical models of the processes. The examples of applications for species separation in biotechnology.	W01-30	MEK01
7	TK02	Chromatographic and adsorptive technique of species separation. Thin layer chromatography, column periodical chromatography and continuous chromatography. The normal and reversed phase chromatography. Ion exchange and gel chromatography. Theory of chromatographic separation: basic mathematical models of adsorption and mass transfer. The influence of process parameters: temperature, composition of mobile phase, solid phase, pH, ion strength of mobile phase on the mixtures separation. The optimization of periodical and continuous process. Principles of selections of chromatographic systems.	W01-30 L01-15	MEK01 MEK02
7	TK03	Drying methods.	W01-30 L01-15	MEK01 MEK02
7	TK04	Crystallization methods - process definition, process statics, crystallization equilibrium, mathematical description and illustration of equilibrium lines, process kinetics, kinetic equations of mass penetration and transfer in crystallization.	W01-30 L01-15	MEK01 MEK02

The student's effort

The type of classes	The work before classes	The participation in classes	The work after classes
Lecture (sem. 7)	The preparation for a test: 2.00 hours/sem.	contact hours: 30.00 hours/sem.	complementing/reading through notes: 2.00 hours/sem.
Laboratory (sem. 7)	The preparation for a Laboratory: 2.00 hours/sem. The preparation for a test: 2.00 hours/sem.	contact hours: 15.00 hours/sem.	Finishing/Making the report: 2.00 hours/sem.
Advice (sem. 7)	The preparation for Advice: 1.00 hours/sem.	The participation in Advice: 1.00 hours/sem.
Credit (sem. 7)	The preparation for a Credit: 2.00 hours/sem.	The written credit: 2.00 hours/sem.

The way of giving the component module grades and the final grade

The type of classes	The way of giving the final grade
Lecture	Passing the Lecture evaluation on the result of the test - OW: Factor related to the term Course test OW: w=1 - 1-st term; w=0.9 - 2-nd term; w=0.8 - 3rd term. w=0.8 - 3-rd term;
Laboratory	Laboratories - lab exercises performed by the group under the supervision of a teacher. The final grade from the laboratory (OL) depends on the total number of points obtained from: the written test at the end of the semester. The condition for passing the laboratory is also correct execution of reports on all exercises. Factor related to the term Course test OL: w=1 - 1-st term; w=0.9 - 2-nd term; w=0.8 - 3rd term. w=0.8 - 3-rd term;
The final grade	FE = 0.7L+0.3Lab.

Sample problems

Required during the exam/when receiving the credit
(-)

Realized during classes/laboratories/projects
(-)

Others
(-)

Can a student use any teaching aids during the exam/when receiving the credit : no

The contents of the module are associated with the research profile: yes

1	D. Antos; R. Muca	Protein association on multimodal chromatography media	2023
2	D. Antos; I. Poplewska; P. Zimoch	Dissociation events during processing of monoclonal antibodies on strong cation exchange resins	2022
3	D. Antos; A. Bajek-Bil; M. Balawejder; M. Olbrycht; W. Piątkowski; I. Poplewska	Development of a Route to the Most Active Nafronyl Stereoisomer by Coupling Asymmetric Synthesis and Chiral Chromatography Separation	2021
4	D. Antos; W. Piątkowski; I. Poplewska	A case study of the mechanism of unfolding and aggregation of a monoclonal antibody in ion exchange chromatography	2021
5	D. Antos; G. Carta; M. Kołodziej; R. Muca; W. Piątkowski	Effects of negative and positive cooperative adsorption of proteins on hydrophobic interaction chromatography media	2020
6	D. Antos; M. Balawejder; H. Lorenz; M. Olbrycht; W. Piątkowski; I. Poplewska; A. Seidel-Morgenstern	Cooperative Kinetic Model to Describe Crystallization in Solid Solution Forming Systems	2019
7	D. Antos; M. Kołodziej; A. Łyskowski; W. Piątkowski; I. Poplewska; P. Szałański	Determination of protein crystallization kinetics by a through-flow small-angle X-ray scattering method	2019