The aim of studying and bibliography

The main aim of study: Understanding of the basics of proteomics and protein engineering used in modern biotechnology

The general information about the module: The module is introduced in the 7th semester of the first degree/ undergraduate course as a mandatory module for the purification and biotechnological products analysis specialty. It is accomplished with 30 hours of lecture and 15 hours of laboratory work. It ends ends with an examination-based assessment.

Bibliography required to complete the module

Bibliography used during lectures

1	Agnieszka Kraj, Anna Drabik, Jerzy Silberring	Proteomika i metabolomika	WUW.	2010
2	Stanisław Ledakiewicz	Inżynieria biochemiczna	Wydawnictwo WNT.	2011,
3	Jin Xiong	Podstawy bioinformatyki	Wydawnictwo Uniwersytetu Warszawskiego .	2009
4	P.C. Turner et al.	Krótkie wykłady: Biologia molekularna	Wydawnictwo Naukowe PWN.	2001
5	B.D. Hames et al.	Krótkie wykłady: Biochemia	Wydawnictwo naukowe PWN.	2002
6	Sawn Doonan	Białka i peptydy	Wydawnictwo Naukowe PWN.	2008
7	Mariusz Jaskólski	Krystalografia dla biologów	Wydawnictwo naukowe UAM.	2010

Bibliography used during classes/laboratories/others

1

Jak dla wykładów

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Bibliography to self-study

1

Jak dla wykładów

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Basic requirements in category knowledge/skills/social competences

Formal requirements: Positively finished courses: biochemistry and molecular biology

Basic requirements in category knowledge: Basic knowledge of biochemistry, genetic engineering and molecular biology as well as (bio)informatics

Basic requirements in category skills: Self-education skills, computer skills

Basic requirements in category social competences: Ability to work both alone and in a group

Module outcomes

MEK	The student who completed the module	Types of classes / teaching methods leading to achieving a given outcome of teaching	Methods of verifying every mentioned outcome of teaching	Relationships with KEK	Relationships with PRK
01	Understands the goal of the protein engineering as well as the tools necessary for its achievement	lecture	written test	K_W05+++ K_W12+++ K_U03++	P6S_UK P6S_WG
02	Knows and understands bioinformatic tools for protein characterisation and its recombinant derivatives	lecture	written test	K_W14+++ K_U03+++	P6S_UK P6S_WG
03	Knows and understands molecular techniques necessary for recombinant protein production	lecture	written test	K_W10+++ K_U18+	P6S_UO P6S_UW P6S_WG
04	Knows and understands isolation, purification and characterisation techniques of recombinant proteins	lecture	written test	K_U09++ K_U19++	P6S_UO P6S_UW
05	Understands the importance of the engineering activities and its influence on the natural environment as well as the responsibilities connected with the decisions made during the process	lecture	written test	K_K02+++	P6S_KO P6S_KR
06	Usage of in-silico methods in protein engineering: -analysis of DNA and protein sequences, -proteomic analysis, -structural analysis.	laboratory	teacher supervision, written report	K_W14+++ K_U09+ K_U18+	P6S_UO P6S_UW P6S_WG

Attention: Depending on the epidemic situation, verification of the achieved learning outcomes specified in the study program, in particular credits and examinations at the end of specific classes, can be implemented remotely (real-time meetings).

The syllabus of the module

Sem.	TK	The content	realized in	MEK
7	TK01	Goal and importance of protein engineering	W01-03	MEK01
7	TK02	Bioinformatic methods in analysis and characterisation of proteins and its recombinant derivatives	W04-09	MEK02 MEK06
7	TK03	Selected aspects of biophysical and biochemical protein characterisation (i) in-silico (ii) by experimental methods	W10-18	MEK03 MEK04
7	TK04	Design and production of recombinant proteins	W19-24	MEK02 MEK04 MEK05 MEK06
7	TK05	Selected aspects of natural and non-natural protein modifications and their importance	W25-30	MEK03 MEK04

The student's effort

The type of classes	The work before classes	The participation in classes	The work after classes
Lecture (sem. 7)		contact hours: 30.00 hours/sem.	complementing/reading through notes: 15.00 hours/sem. Studying the recommended bibliography: 15.00 hours/sem.
Laboratory (sem. 7)	The preparation for a Laboratory: 3.00 hours/sem. The preparation for a test: 5.00 hours/sem.	contact hours: 15.00 hours/sem.	Finishing/Making the report: 5.00 hours/sem.
Advice (sem. 7)
Credit (sem. 7)	The preparation for a Credit: 15.00 hours/sem.	The written credit: 2.00 hours/sem.

The way of giving the component module grades and the final grade

The type of classes	The way of giving the final grade
Lecture	The note is proportional to the number of points earned during exam and follows criteria of the 'Wydziałowa Księga Jakości Kształcenia'. The positive note is achived only when 50% primary points is collected.
Laboratory	Pass note is granted to the students who participated in all laboratory exercises and presented written report based on performed exercises.
The final grade	The final note is an avarage of all notes collected from the modules: K=a*wA+c*wC+l*wL (a-lecture coeff., c-tutorial coeff., l-laboratory coeff., w=1,0, w=0,9, w=0,8 receptively for the 1st, 2nd, 3rd attempt). The note is calculated only if all components have been passed. Values of coefficiants are presented to the students during the initial teaching session.

Sample problems

Required during the exam/when receiving the credit
(-)

Realized during classes/laboratories/projects
(-)

Others
(-)

Can a student use any teaching aids during the exam/when receiving the credit : no

The contents of the module are associated with the research profile: yes

1	M. Dżugan; A. Łyskowski; M. Miłek	Assessing the Antimicrobial Properties of Honey Protein Components through In Silico Comparative Peptide Composition and Distribution Analysis	2023
2	V. Csitkovits; K. Gruber; C. Kratky; B. Kräutler; A. Łyskowski	Structure-Based Demystification of Radical Catalysis by a Coenzyme B12 dependent Enzyme – Crystallographic Study of Glutamate Mutase with Cofactor Homologues	2022
3	K. Chen; Ł. Jaremko; M. Jaremko; A. Łyskowski	Genetic and Molecular Factors Determining Grain Weight in Rice	2021
4	D. Antos; M. Kołodziej; A. Łyskowski; W. Piątkowski; I. Poplewska; P. Szałański	Determination of protein crystallization kinetics by a through-flow small-angle X-ray scattering method	2019