Item card
Proteomics and protein engineering
Some basic information about the module
Cycle of education:
2022/2023
The name of the faculty organization unit:
The faculty Chemistry
The name of the field of study:
Biotechnology
The area of study:
technical sciences
The profile of studing:
The level of study:
first degree study
Type of study:
full time
discipline specialities :
Applied biochemistry, Purification and analysis of biotechnological products
The degree after graduating from university:
Bachelor of Science (BSc)
The name of the module department :
Department of Biochemistry and Bioinformatics
The code of the module:
241
The module status:
mandatory for the speciality Purification and analysis of biotechnological products
The position in the studies teaching programme:
sem: 7 / W30 L15 / 4 ECTS / Z
The language of the lecture:
Polish
The name of the coordinator:
Andrzej Łyskowski, PhD, Eng.
The aim of studying and bibliography
The main aim of study:
Understanding of the basics of proteomics and protein engineering used in modern biotechnology
The general information about the module:
The module is introduced in the 7th semester of the first degree/ undergraduate course as a mandatory module for the purification and biotechnological products analysis specialty. It is accomplished with 30 hours of lecture and 15 hours of laboratory work. It ends ends with an examination-based assessment.
Bibliography required to complete the module
| 1 | Agnieszka Kraj, Anna Drabik, Jerzy Silberring | Proteomika i metabolomika | WUW. | 2010 |
| 2 | Stanisław Ledakiewicz | Inżynieria biochemiczna | Wydawnictwo WNT. | 2011, |
| 3 | Jin Xiong | Podstawy bioinformatyki | Wydawnictwo Uniwersytetu Warszawskiego . | 2009 |
| 4 | P.C. Turner et al. | Krótkie wykłady: Biologia molekularna | Wydawnictwo Naukowe PWN. | 2001 |
| 5 | B.D. Hames et al. | Krótkie wykłady: Biochemia | Wydawnictwo naukowe PWN. | 2002 |
| 6 | Sawn Doonan | Białka i peptydy | Wydawnictwo Naukowe PWN. | 2008 |
| 7 | Mariusz Jaskólski | Krystalografia dla biologów | Wydawnictwo naukowe UAM. | 2010 |
| 1 | Jak dla wykładów | - | -. | - |
| 1 | Jak dla wykładów | - | -. | - |
Basic requirements in category knowledge/skills/social competences
Formal requirements:
Positively finished courses: biochemistry and molecular biology
Basic requirements in category knowledge:
Basic knowledge of biochemistry, genetic engineering and molecular biology as well as (bio)informatics
Basic requirements in category skills:
Self-education skills, computer skills
Basic requirements in category social competences:
Ability to work both alone and in a group
Module outcomes
| MEK | The student who completed the module | Types of classes / teaching methods leading to achieving a given outcome of teaching | Methods of verifying every mentioned outcome of teaching | Relationships with KEK | Relationships with PRK |
|---|---|---|---|---|---|
| MEK01 | Understands the goal of the protein engineering as well as the tools necessary for its achievement | lecture | written test |
K-W05+++ K-W12+++ K-U03++ |
P6S-UK P6S-WG |
| MEK02 | Knows and understands bioinformatic tools for protein characterisation and its recombinant derivatives | lecture | written test |
K-W14+++ K-U03+++ |
P6S-UK P6S-WG |
| MEK03 | Knows and understands molecular techniques necessary for recombinant protein production | lecture | written test |
K-W10+++ K-U18+ |
P6S-UO P6S-UW P6S-WG |
| MEK04 | Knows and understands isolation, purification and characterisation techniques of recombinant proteins | lecture | written test |
K-U09++ K-U19++ |
P6S-UO P6S-UW |
| MEK05 | Understands the importance of the engineering activities and its influence on the natural environment as well as the responsibilities connected with the decisions made during the process | lecture | written test |
K-K02+++ |
P6S-KO P6S-KR |
| MEK06 | Usage of in-silico methods in protein engineering: -analysis of DNA and protein sequences, -proteomic analysis, -structural analysis. | laboratory | teacher supervision, written report |
K-W14+++ K-U09+ K-U18+ |
P6S-UO P6S-UW P6S-WG |
The syllabus of the module
| Sem. | TK | The content | realized in | MEK |
|---|---|---|---|---|
| 7 | TK01 | W01-03 | MEK01 | |
| 7 | TK02 | W04-09 | MEK02 MEK06 | |
| 7 | TK03 | W10-18 | MEK03 MEK04 | |
| 7 | TK04 | W19-24 | MEK02 MEK04 MEK05 MEK06 | |
| 7 | TK05 | W25-30 | MEK03 MEK04 |
The student's effort
| The type of classes | The work before classes | The participation in classes | The work after classes |
|---|---|---|---|
| Lecture (sem. 7) | contact hours:
30.00 hours/sem. |
complementing/reading through notes:
15.00 hours/sem. Studying the recommended bibliography: 15.00 hours/sem. |
|
| Laboratory (sem. 7) | The preparation for a Laboratory:
3.00 hours/sem. The preparation for a test: 5.00 hours/sem. |
contact hours:
15.00 hours/sem. |
Finishing/Making the report:
5.00 hours/sem. |
| Advice (sem. 7) | |||
| Credit (sem. 7) | The preparation for a Credit:
15.00 hours/sem. |
The written credit:
2.00 hours/sem. |
The way of giving the component module grades and the final grade
| The type of classes | The way of giving the final grade |
|---|---|
| Lecture | The note is proportional to the number of points earned during exam and follows criteria of the 'Wydziałowa Księga Jakości Kształcenia'. The positive note is achived only when 50% primary points is collected. |
| Laboratory | Pass note is granted to the students who participated in all laboratory exercises and presented written report based on performed exercises. |
| The final grade | The final note is an avarage of all notes collected from the modules: K=a*wA+c*wC+l*wL (a-lecture coeff., c-tutorial coeff., l-laboratory coeff., w=1,0, w=0,9, w=0,8 receptively for the 1st, 2nd, 3rd attempt). The note is calculated only if all components have been passed. Values of coefficiants are presented to the students during the initial teaching session. |
Sample problems
Required during the exam/when receiving the credit
(-)
Realized during classes/laboratories/projects
(-)
Others
(-)
Can a student use any teaching aids during the exam/when receiving the credit : no
The contents of the module are associated with the research profile yes
| 1 | A. Bocian; A. Łyskowski; B. Marczak | Antimicrobial Peptide Databases as the Guiding Resource in New Antimicrobial Agent Identification via Computational Methods | 2025 |
| 2 | A. Bocian; J. Buczkowicz; E. Ciszkowicz; K. Hus; K. Lecka-Szlachta; A. Łyskowski | Peptyd antybakteryjny oraz jego zastosowanie | 2025 |
| 3 | A. Bocian; J. Buczkowicz; E. Ciszkowicz; K. Hus; K. Lecka-Szlachta; A. Łyskowski | Peptyd antybakteryjny oraz zastosowanie tego peptydu antybakteryjnego | 2025 |
| 4 | E. Ciszkowicz; M. Dżugan; A. Łyskowski; M. Miłek; M. Tomczyk | Selected Polyphenols of Polish Poplar Propolis as a Key Component Shaping Its Antibacterial Properties—In Vitro and In Silico Approaches | 2025 |
| 5 | M. Bauer; A. Bocian; J. Buczkowicz; E. Ciszkowicz; K. Hus; W. Kamysz; K. Lecka-Szlachta; A. Łyskowski; A. Miłoś; D. Neubauer; A. Nieczaj; K. Sikora | AMPEC4: Naja ashei Venom-Derived Peptide as a Stimulator of Fibroblast Migration with Antibacterial Activity | 2025 |
| 6 | M. Dąbrowska; A. Łyskowski; M. Misiorek; Ż. Szymaszek; M. Twardowska; Ł. Uram; S. Wołowiec | Human Embryonic Kidney HEK293 Cells as a Model to Study SMVT-Independent Transport of Biotin and Biotin-Furnished Nanoparticles in Targeted Therapy | 2025 |
| 7 | A. Łyskowski; M. Misiorek; Z. Rauk; Z. Setkowicz; Ż. Szymaszek; M. Twardowska; Ł. Uram; S. Wołowiec | The Importance of Biotinylation for the Suitability of Cationic and Neutral Fourth-Generation Polyamidoamine Dendrimers as Targeted Drug Carriers in the Therapy of Glioma and Liver Cancer | 2024 |
| 8 | M. Dżugan; A. Łyskowski; M. Miłek | Assessing the Antimicrobial Properties of Honey Protein Components through In Silico Comparative Peptide Composition and Distribution Analysis | 2023 |
| 9 | V. Csitkovits; K. Gruber; C. Kratky; B. Kräutler; A. Łyskowski | Structure-Based Demystification of Radical Catalysis by a Coenzyme B12 dependent Enzyme – Crystallographic Study of Glutamate Mutase with Cofactor Homologues | 2022 |
| 10 | K. Chen; Ł. Jaremko; M. Jaremko; A. Łyskowski | Genetic and Molecular Factors Determining Grain Weight in Rice | 2021 |