The aim of studying and bibliography

The main aim of study: Acquisition of a theoretical knowledge and experience on practical applications of modern instrumental analysis techniques used for chemical analysis of organic and inorganic compounds.

The general information about the module: The module is realised in the 5-th semester and include 30 hours of lectures and 45 hours of laboratory practice. Module ends with a graded assignment.

others: Instrukcje do ćwiczeń laboratoryjnych

Bibliography required to complete the module

Bibliography used during lectures

1	K. Danzer i inn.	Analityka – przegląd systematyczny	WNT, Warszawa .	1993
2	A. Cygański	Metody spektroskopowe w chemii analitycznej	WNT, Warszawa.	1997
3	E. Hoffmann, J. Charette, V. Stroobant	Spektrometria mas	WNT, Warszawa.	1998
4	A. Cygański	Metody elektroanalityczne	WNT, Warszawa.	1995
5	Z. Witkiewicz	Podstawy chromatografii	WNT, Warszawa.	2005

Bibliography used during classes/laboratories/others

1	W. Szczepaniak	Metody instrumentalne w analizie chemicznej	PWN, Warszawa.	2004
2	R.M. Silverstein i inn.	Spektroskopowe metody identyfikacji związków organicznych	PWN, Warszawa.	2007

Bibliography to self-study

1

W. Szczepaniak

Metody instrumentalne w analizie chemicznej

PWN, Warszawa.

2004

Basic requirements in category knowledge/skills/social competences

Formal requirements: Registration for V semester

Basic requirements in category knowledge: Knowledge and understanding of the basic physics, general, analytical, organic and physical chemistry.

Basic requirements in category skills: Skills in calculation of chemical concentration, solutions preparation, operation of basic laboratory equipment for chemical analysis, measurement as well as computer. A basic knowledge of mathematics

Basic requirements in category social competences: Knowledge of principles of safe work in chemical laboratory, responsibility during experiments performed individually or in laboratory group.

Module outcomes

MEK	The student who completed the module	Types of classes / teaching methods leading to achieving a given outcome of teaching	Methods of verifying every mentioned outcome of teaching	Relationships with KEK	Relationships with PRK
01	Has a theoretical and practical knowledge of construction and mechanizm of action of modern analytical equipment.	lecture, laboratory	written test, performance observation	K_W04++	P6S_WG
02	Has a basic knowledge of the possibilities and application of particular instrumental methods for solving analytical problems.	lecture	written test	K_W04++	P6S_WG
03	Knows basic concepts connected with the instrumental methods used for analysis of the materials and organic compounds.	lecture	written test	K_W04++	P6S_WG
04	Is able to justify the choice of a particular instrumental method suitable to the analytical and economical conditions.	lecture, laboratory	written test	K_U11++ K_U21+	P6S_UO P6S_UW
05	Is able to make use of analytical instruments to perform individual types of analysis.	laboratory	written test, observation of job performance	K_U21++	P6S_UW
06	Can select the appropriate calibration method; carry out identification and elimination of measurement errors.	lecture, laboratory	written test	K_U11+	P6S_UO P6S_UW
07	Is able to prepare a report on the results of the investigation, perform interpretation and evaluation of the analysis results and draw correct conclusions.	laboratory	written report	K_U21+	P6S_UW
08	Is abble to work individually and in a team environment in laboratory of instrumental analysis.	laboratory	observation of job performance	K_U21+	P6S_UW
09	Is able to identify a situation threatening to life or health and follow the principles of safe work in chemical laboratory.	laboratory	observation of job performance	K_U14+	P6S_UW
10	Understand the need of widening their knowledge in the field of new solutions related to equipment and methods of instrumental analysis.	lecture, laboratory	observation of job performance	K_K01++	P6S_KK P6S_KO P6S_KR

Attention: Depending on the epidemic situation, verification of the achieved learning outcomes specified in the study program, in particular credits and examinations at the end of specific classes, can be implemented remotely (real-time meetings).

The syllabus of the module

Sem.	TK	The content	realized in	MEK
5	TK01	Analytical process, its elements and statistical evaluation of each step. Analysis of elements and compounds by spectroscopic methods. Atomic Emission Spectroscopy - basis of the method, methods of sample atomization and excitation, applications. Atomic absorption spectroscopy. Molecular spectroscopy in the ultraviolet and visible light. Infrared spectroscopy. Spectra recording techniques, methods of quantitative and qualitative analysis. Fundamentals of nuclear magnetic resonance spectroscopy. The quantitative and structural analysis based on the NMR spectra. Fundamentals of mass spectrometry. Interpretation and application of analytical mass spectra for organic compounds. Chromatographic methods for separation of mixtures. Basic principles and classification. Theoreticasl basis of separation process. Retention mechanisms and parameters. Separation efficiency. Definition and determination of resolution index, theoretical plate number, selectivity factor. Separation techniques in liquid chromatography - adsorption chromatography, partition - normal/reverse chromatography, ion-exchange chromatography , gel filtration chromatography. Selection of the chromatographic conditions - rules for the selection of the stationary and mobile phases. High Performance Liquid Chromatography HPLC and thin-layer HPTLC. Isocratic and gradient techniques, instrumentation. Gas chromatography. The rate theory of chromatography - band broadening, column efficiency. Optimization of column performance. Chromatographic methods of qualitative and quantitative analysis. Potentiometric methods. Design, operation and application of the selected ion-selective electrodes. Conductometry and its analytical application. Voltammetric methods - linear-sweep LSV, cyclic CV, and stripplng CSV, ASV techniques. Quantitative and qualitative analysis. Selected applications in analytical laboratory and industrial applications, criteria for the method selection.	W30	MEK01 MEK02 MEK03 MEK04 MEK06 MEK10
5	TK02	1.Gas Chromatography - Identification of components in a mixture of hydrocarbons. Quantitative determination of the substance content in a multi-component mixture. 2. Liquid chromatography - Determination of caffeine using liquid chromatography. 3.Mass spectrometry. Analysis of the composition of the mixture of hydrocarbons using gas chromatography coupled with mass spectrometry. 4. Analysis of the structure of organic compounds by IR spectroscopy. Basic rules for the interpretation of IR spectra. Methods of sample preparation in IR spectroscopy. 5. Determination of the parameters of the absorption band and the molar absorption coefficient. Quantitative determination of the content of picric acid in the test sample. 6. Analysis of 1H-NMR spectra. 7.Determination of the content of elements in solutions by atomic absorption spectroscopy (AAS). 8. Polarimetric determination of sucrose concentration in aqueous solutions. 9. Quantitative determination of paracetamol content by cyclic voltammetry. 10. Determination of the concentration of iodides and chlorides side by side by potentiometric precipitation titration. 11. Determination of weak acid concentration by conductometric titration.	L45	MEK01 MEK04 MEK05 MEK07 MEK08 MEK09 MEK10

The student's effort

The type of classes	The work before classes	The participation in classes	The work after classes
Lecture (sem. 5)		contact hours: 30.00 hours/sem.	complementing/reading through notes: 3.00 hours/sem. Studying the recommended bibliography: 10.00 hours/sem.
Laboratory (sem. 5)	The preparation for a Laboratory: 2.00 hours/sem. The preparation for a test: 30.00 hours/sem.	contact hours: 45.00 hours/sem.	Finishing/Making the report: 15.00 hours/sem.
Advice (sem. 5)		The participation in Advice: 2.00 hours/sem.
Credit (sem. 5)	The preparation for a Credit: 15.00 hours/sem.	The written credit: 2.00 hours/sem.

The way of giving the component module grades and the final grade

The type of classes	The way of giving the final grade
Lecture	A written test including the content of lectures and laboratories. The test includes theoretical part and calculation problems. The mark (OW) depends on the score gained: 3.0 (50.0%-60.0%) MP ; 3.5 (60.1%-70.0%) MP; 4.0 (70.1%-80.0%) MP; 4,5 (80.1%-90.0%) MP; 5.0 (90.1%-100%) MP. MP denotes the full score.
Laboratory	The necessary condition for receiving a credit for the laboratory is obtaining at least a passing mark on each exercise included in the schedule. The mark obtained during each laboratory exercise is an arithmetic mean of the marks for a written/oral test, correct performance of an experiment and correct preparation of a report. The total mark in the laboratory work (OL) is calculated as arithmetic mean of the marks obtained for every exercise included in the schedule. A final mark for the laboratory is rounded according to WKZJK.
The final grade	The final mark in the module (K) is calculated according to the formula: K= w 0,5 OW + w 0,5 OL; where: OW, OL denote positive marks for lecture test and laboratory practice, respectively, w - coefficient for delay, w =1.0 when a passing mark is obtained in due time, w=0.9 for a first resit, w=0.8 for a second resit. The final mark is rounded according to WKZJK.

Sample problems

Required during the exam/when receiving the credit
(-)

Realized during classes/laboratories/projects
(-)

Others
(-)

Can a student use any teaching aids during the exam/when receiving the credit : no

The contents of the module are associated with the research profile: yes

1	D. Naróg; A. Sobkowiak	Electrochemistry of Flavonoids	2023
2	D. Naróg; A. Sobkowiak	Electrochemical Investigation of some Flavonoids in Aprotic Media	2022
3	D. Naróg	Electrochemical study of quercetin in the presence of galactopyranose: Potential application to the electrosynthesis of glycoconjugates of quinone/quinone methide of quercetin	2020