The aim of studying and bibliography

The main aim of study: Familiarization with basic principles of drugs action at molecular level, classical methods of molecular farmacology and new trends in pharmacology, pharmacogenetics ang pharmacogenomics.

The general information about the module: The module is introduced in the 2nd semester of the second degree course as a mandatory module for the speciality. It is accomplished with 30 hours of lectures and 15 hours of laboratory work.

Teaching materials: Strona internetowa dla studentów: http://www.nrl.uvlf.sk/prz/

Bibliography required to complete the module

Bibliography used during lectures

1	Rosypal S.	Úvod do molekulární biologie (rozdziały 1-4)	Brno.	2002
2	Křemen J. et al.	Techniky molekulární biologie a jejich využití v medicíně. Skriptum UK 1.LF	Karolinum, Praha.	1998
3	Kalow, Meyer, Tyndale	Pharmacogenomics	Marcel Dekker.	2001

Bibliography used during classes/laboratories/others

1

J. Legáth

Ćwiczenia - Molekularne podstawy farmakologii

http://www.nrl.uvlf.sk/prz/cwmf.html.

2013

Basic requirements in category knowledge/skills/social competences

Formal requirements: Positively finished courses: Immunological techniques in biotechnology, Biochemistry, Enzymology

Basic requirements in category knowledge: Basic knowledge of biochemistry, immunology and enzymology

Basic requirements in category skills: Self-education and searching for information skills.

Basic requirements in category social competences: Individual and team work skills.

Module outcomes

MEK	The student who completed the module	Types of classes / teaching methods leading to achieving a given outcome of teaching	Methods of verifying every mentioned outcome of teaching	Relationships with KEK	Relationships with PRK
01	Knows basic terminology and properly uses pharmacological nomenclature.	lecture	written test	K_W03++	P7S_WG
02	Has a basic knowledge of the drug development process and using of natural drugs and biopharmaceuticals	lecture	written test	K_W03++ K_U15++	P7S_UW P7S_WG
03	Has a basic knowledge about causes of diseases.	lecture	written test	K_W03+++	P7S_WG
04	Knows basic molecular targets of drugs.	lecture	written test	K_W03+++	P7S_WG
05	Knows fundamental research methods in molecular oharmacology.	lecture, laboratory work	written test	K_W03+++ K_U15++	P7S_UW P7S_WG
06	Knows new trends and ethical issues of contemporary pharmacology.	lecture, laboratory work	written test	K_W03+++ K_K03+++	P7S_KR P7S_WG

Attention: Depending on the epidemic situation, verification of the achieved learning outcomes specified in the study program, in particular credits and examinations at the end of specific classes, can be implemented remotely (real-time meetings).

The syllabus of the module

Sem.	TK	The content	realized in	MEK
2	TK01	Introduction in to molecular pharmacology, definition, terminology, methodology and introduction on the drug development.	W01	MEK01 MEK02
2	TK02	Live cycle of prokaryotic and eukaryotic cells, mechanisms of regulatory, apoptosis and necrosis	W02	MEK03
2	TK03	Cells parasites: viruses, bacteriophage, his function in the science and biotechnology. Prions diseases.	W03	MEK03
2	TK04	Mechanism of action of antivirotics, classify of antivirotics, desinficiens and resistance.	W04	MEK03 MEK04
2	TK05	Drugs and cell membrane, mechanisms of transporting, mechanisms of signal transduction, ionic canals, chemoreceptors, classify of receptors, receptor theory.	W05	MEK04
2	TK06	The basis of enzymology. Enzymes of nuclei, mitochondrial enzymes, extra cellular enzymes and metabolism of drugs, pharmacogenetics and pharmacogenomics.	W06	MEK01 MEK04
2	TK07	Cells organization, tissue and organs, organs system, mechanisms of cell differentiation. Transmission of signal between cells, potential of action, mediators, synapses. Influence of formation and transmission of excitement with drugs – mechanism of action.	W07	MEK03 MEK04
2	TK08	Mutagenity, carcinogenity, tumor, mechanism of action of cytostatics, resistance.	W08	MEK03 MEK04
2	TK09	Structure of proteins and utilization of change of structure of proteins to development of new drugs. Value of protein p53.	W09	MEK02 MEK03 MEK04
2	TK10	Molecular base of choice pathophysiological state (neurogenerative diseases, cardiovascular diseases etc.) – molecular influence this states with drugs.	W10	MEK03 MEK04
2	TK11	Molecular base of immunofarmacology and immunotoxicology, inflammation, allergy, Drugs, which influence immune system.	W11	MEK01 MEK03 MEK04
2	TK12	Natural drugs of plants or animal origin – preparation and separation, mechanism of molecular action in the organisms.	W12	MEK02
2	TK13	Application and use molecular methods in the pharmacology - biopharmaceuticals.	W13	MEK02 MEK06
2	TK14	GMO and their products and ethic problem of molecular biology and pharmacology.	W14	MEK01 MEK06
2	TK15	Cells culture – cultivate, reproduce and preparation for testing of new anti-tumors drugs	L01	MEK05 MEK06
2	TK16	Hystochemical methods – preparation, methods of coloring, determination of intracellular enzymes in the tissues	L02	MEK05
2	TK17	Modeling of molecular membrane effect and membrane mode of action of synthetic and natural drugs	L03	MEK04 MEK05
2	TK18	Methodology and methods of pharmacogenetics and pharmacogenomics – modeling of enzymobiotic and others biological active proteins (model exercise)	L04	MEK05 MEK06

The student's effort

The type of classes	The work before classes	The participation in classes	The work after classes
Lecture (sem. 2)		contact hours: 30.00 hours/sem.	complementing/reading through notes: 5.00 hours/sem.
Laboratory (sem. 2)	The preparation for a Laboratory: 4.00 hours/sem. The preparation for a test: 8.00 hours/sem.	contact hours: 15.00 hours/sem.
Advice (sem. 2)		The participation in Advice: 2.00 hours/sem.
Credit (sem. 2)	The preparation for a Credit: 12.00 hours/sem.	The written credit: 1.00 hours/sem.

The way of giving the component module grades and the final grade

The type of classes	The way of giving the final grade
Lecture	test, examination
Laboratory	written test
The final grade	The final note (OK) is calculated from written test including issues from lectures and laboratory exercises. 100 points; 51-60p. - 3.0; 61-70p. - 3.5; 71-80p. - 4.0; 81-90p. - 4.5; 91-100p. - 5.0

Sample problems

Required during the exam/when receiving the credit
(-)

Realized during classes/laboratories/projects
(-)

Others
(-)

Can a student use any teaching aids during the exam/when receiving the credit : no

The contents of the module are associated with the research profile: yes

1	A. Bocian; J. Buczkowicz; K. Hus; J. Legath; T. Litschka-Koen; V. Petrilla; M. Petrillova; M. Pietrowska	Venom diversity in Naja mossambica: Insights from proteomic and immunochemical analyses reveal intraspecific differences	2024
2	B. Bakera; M. Rakoczy-Trojanowska; M. Szeliga; M. Święcicka; M. Tyrka	Identification of candidate genes responsible for chasmogamy in wheat	2023
3	P. Bednarek; A. Dorczyk; T. Drzazga; D. Jasińska; P. Krajewski; B. Ługowska; R. Martofel; P. Matysik; M. Niewińska; D. Ratajczak; K. Rączka; T. Sikora; D. Tyrka; M. Tyrka; E. Witkowski; U. Woźna-Pawlak	Genome-wide association mapping in elite winter wheat breeding for yield improvement	2023
4	A. Bocian; J. Gikunju; K. Hus; J. Kimotho ; J. Legath; E. Manson; K. Mutinda; V. Petrilla	Development of an Inhibition Enzyme-Linked Immunosorbent Assay (ELISA) Prototype for Detecting Cytotoxic Three-Finger Toxins (3FTxs) in African Spitting Cobra Venoms	2022
5	A. Bocian; K. Hus; J. Kimani; J. Kimotho ; M. Kyama; J. Legath; E. Manson; V. Petrilla	Development and Characterization of Anti-Naja ashei Three-Finger Toxins (3FTxs)-Specific Monoclonal Antibodies and Evaluation of Their In Vitro Inhibition Activity	2022
6	M. Dyda; G. Gołębiowska; M. Rapacz; M. Szechyńska-Hebda; M. Tyrka; I. Wąsek; M. Wędzony	Quantitative trait loci and candidate genes associated with freezing tolerance of winter triticale (× Triticosecale Wittmack)	2022
7	M. Dyda; G. Gołębiowska; M. Rapacz; M. Tyrka; M. Wędzony	Genetic mapping of adult-plant resistance genes to powdery mildew in triticale	2022
8	M. Dyda; G. Gołębiowska; M. Rapacz; M. Tyrka; M. Wędzony	Mapping of QTL and candidate genes associated with powdery mildew resistance in triticale (× Triticosecale Wittm.)	2022
9	P. Krajewski; R. Marcinkowski; R. Martofel; P. Matysik; M. Mokrzycka; M. Rakoczy-Trojanowska; M. Rokicki; S. Stojałowski; M. Tyrka; U. Woźna-Pawlak; B. Żmijewska	Genome-Wide Association Analysis for Hybrid Breeding in Wheat	2022
10	A. Barbasz; A. Bocian; A. Czyżowska; B. Dyba; K. Hus; J. Legath; V. Petrilla; M. Petrillova; E. Rudolphi-Szydło	Effects of 3FTx Protein Fraction from Naja ashei Venom on the Model and Native Membranes: Recognition and Implications for the Mechanisms of Toxicity	2021
11	A. Pietrusińska; M. Tyrka	Linkage of Lr55 wheat leaf rust resistance gene with microsatellite and DArT-based markers	2021
12	B. Bakera; P. Krajewski; M. Mokrzycka; M. Rakoczy-Trojanowska; M. Szeliga; M. Święcicka; M. Tyrka	Identification of Rf Genes in Hexaploid Wheat (Triticumaestivum L.) by RNA-Seq and Paralog Analyses	2021
13	B. Bakera; P. Krajewski; P. Matysik; M. Mokrzycka; M. Rakoczy-Trojanowska; M. Rokicki; S. Stojałowski; M. Szeliga; D. Tyrka; M. Tyrka	Evaluation of genetic structure in European wheat cultivars and advanced breeding lines using high-density genotyping-by-sequencing approach	2021
14	J. Buczkowicz; T. Drzazga; B. Ługowska; P. Matysik; K. Rubrycki; M. Semik; D. Tyrka; M. Tyrka; E. Witkowski	Identyfikacja efektywnych genów odporności na wybrane choroby wirusowe i grzybowe pszenicy zwyczajnej	2021
15	J. Buczkowicz; T. Drzazga; G. Fic; M. Jaromin; P. Krajewski; P. Matysik; R. Mazur; P. Milczarski; T. Sikora; M. Szeliga; D. Tyrka; M. Tyrka; E. Witkowski	Selekcja genomowa pszenicy ozimej	2021
16	A. Bocian; J. Buczkowicz; E. Ciszkowicz; K. Hus; K. Lecka-Szlachta; J. Legath; L. Legath; V. Petrilla; M. Petrillova; M. Pietrowska	Antimicrobial Activity of Protein Fraction from Naja ashei Venom Against Staphylococcus epidermidis	2020
17	A. Bocian; J. Buczkowicz; K. Hus; M. Jaromin; J. Legath; D. Łysiak; V. Petrilla; M. Petrillova; S. Sławek	Comparison of Methods for Measuring Protein Concentration in Venom Samples	2020
18	A. Bocian; K. Hus; E. Kuna; J. Legath; A. Lewińska; V. Petrilla; M. Petrillova; M. Wnuk	Evaluation of Antifungal Activity of Naja pallida and Naja mossambica Venoms against Three Candida Species	2020
19	A. Bocian; K. Hus; J. Legath; Ł. Marczak; V. Petrilla; M. Petrillova	Different research approaches in unraveling the venom proteome of Naja ashei	2020
20	E. Ciszkowicz; E. Kaznowska; P. Porzycki; M. Semik; M. Tyrka	MiR-93/miR-375: Diagnostic Potential, Aggressiveness Correlation and Common Target Genes in Prostate Cancer	2020
21	G. Czajowski; M. Karbarz; M. Pojmaj; A. Strzembicka; D. Tyrka; M. Tyrka; A. Wardyńska; M. Wędzony	Quantitative trait loci mapping of adult-plant resistance to powdery mildew in triticale	2020
22	J. Ciura; M. Szeliga; M. Tyrka	Representational Difference Analysis of Transcripts Involved in Jervine Biosynthesis	2020
23	A. Bocian; J. Buczkowicz; K. Hus; M. Jaromin; J. Legath	An effective method of isolating honey proteins	2019
24	J. Ciura; M. Grzesik; M. Szeliga; M. Tyrka	Identification of candidate genes involved in steroidal alkaloids biosynthesis in organ-specific transcriptomes of Veratrum nigrum L.	2019
25	J. Legath; D. Marcincakova; M. Miłek	Polyphenols Content, Antioxidant Activity, and Cytotoxicity Assessment of Taraxacum officinale Extracts Prepared through the Micelle-Mediated Extraction Method	2019
26	M. Dyda; M. Szechyńska-Hebda; M. Tyrka; I. Wąsek; M. Wędzony	Local and systemic regulation of PSII efficiency in triticale infected by the hemibiotrophic pathogen Microdochium nivale	2019
27	M. Dziurka; K. Hura; T. Hura; A. Ostrowska; M. Tyrka	Participation of Wheat and Rye Genome in Drought Induced Senescence in Winter Triticale (X Triticosecale Wittm.)	2019
28	T. Csank; M. Falis; M. Fedorova; K. Hus; J. Legath; S. Marcincak; D. Marcincakova; D. Mudronova; P. Schusterova	Impact of Zinc Sulfate Exposition on Viability, Proliferation and Cell Cycle Distribution of Epithelial Kidney Cells	2019
29	Z. Banaszak; A. Fiust; Z. Nita; W. Orłowska-Job; M. Pojmaj; M. Rapacz; M. Tyrka; M. Wójcik-Jagła	Sposób selekcji mrozoodpornych genotypów jęczmienia ozimego	2019