The aim of studying and bibliography

The main aim of study: Familiarize students with the latest technologies used in genome and proteome research

The general information about the module: The module is introduced in the 2nd semester of the second degree/ graduate course as a faculty module for specialty.It is accomplished with 15 hours of lecture and 30 hours of laboratory work.

Bibliography required to complete the module

Bibliography used during lectures

1	Nedelkov D. Nelson R. W.	New and Emerging Proteomic Techniques	Humana Press, New Jersey, USA.	2006
2	Harding S.E.	Biotechnology and Genetic Engineering Reviews	Nottingham University Press.	2008
3	Mitchelson K.R.	New high throughput technologies for DNA sequencing and genomics	Elsevier .	2007

Basic requirements in category knowledge/skills/social competences

Formal requirements: Valid registration

Basic requirements in category knowledge: Basic knowledge of protein and nucleic acids structure

Basic requirements in category skills: Self-education skills

Basic requirements in category social competences: Ability to work both alone and in a group

Module outcomes

MEK	The student who completed the module	Types of classes / teaching methods leading to achieving a given outcome of teaching	Methods of verifying every mentioned outcome of teaching	Relationships with KEK	Relationships with PRK
01	Has knowledge about DNA and RNA technologies used in molecular biology	lecture	written test	K_W06+++ K_W07++ K_U05++	P7S_UU P7S_WG
02				K_U09++ K_U10++ K_U11++ K_K02++	P7S_KR P7S_UW
03	Has knowledge about proteomic technologies as well as those using antibidies	lecture	written test	K_W06+++ K_W07+++ K_U05++ K_U09++	P7S_UU P7S_UW P7S_WG
04	Is able to choose proper methodology to resolve the problems in the field of molecular biology	lecture	writen test	K_W06+++ K_W07+++ K_U09+++ K_U10+++	P7S_UW P7S_WG

Attention: Depending on the epidemic situation, verification of the achieved learning outcomes specified in the study program, in particular credits and examinations at the end of specific classes, can be implemented remotely (real-time meetings).

The syllabus of the module

Sem.	TK	The content	realized in	MEK
2	TK01	Protein isolation, two-dimensional electrophoresis, protein identification techniques	L	MEK02 MEK03
2	TK02	DNA- and RNA-based technologies	W1-W5	MEK01 MEK03 MEK04
2	TK03	Ig-based technologies	W6-W8	MEK01 MEK03 MEK04
2	TK04	Proteomic techniques	W9-W11	MEK01 MEK03
2	TK05	Recombinant proteins and protein engineering	W12-W14	MEK01 MEK03 MEK04
2	TK06	Transgenic organisms	W15	MEK01 MEK03 MEK04

The student's effort

The type of classes	The work before classes	The participation in classes	The work after classes
Lecture (sem. 2)		contact hours: 15.00 hours/sem.
Laboratory (sem. 2)	The preparation for a test: 15.00 hours/sem.	contact hours: 30.00 hours/sem.
Advice (sem. 2)		The participation in Advice: 2.00 hours/sem.
Credit (sem. 2)	The preparation for a Credit: 15.00 hours/sem.	The written credit: 1.00 hours/sem.

The way of giving the component module grades and the final grade

The type of classes	The way of giving the final grade
Lecture	The grade is issued in a manner appropriate to the number of points received during the exam: 50-59% - [3], 60-69% - [3,5], 70-79% - [4], 80-89 - [4,5], 90-100 - [5]
Laboratory	The grade is issued on the basis of the average grade of 5 tests. Test points: 50-59% - [3], 60-69% - [3,5], 70-79% - [4], 80-89 - [4,5, 90-100 - [5]
The final grade	The final grade is the average of the grade of lectures and a final evaluation labs (50/50). The average may be drawn only if both grades are positive

Sample problems

Required during the exam/when receiving the credit
(-)

Realized during classes/laboratories/projects
(-)

Others
(-)

Can a student use any teaching aids during the exam/when receiving the credit : no

The contents of the module are associated with the research profile: yes

1	A. Bocian; J. Buczkowicz; K. Hus; J. Legath; T. Litschka-Koen; V. Petrilla; M. Petrillova; M. Pietrowska	Venom diversity in Naja mossambica: Insights from proteomic and immunochemical analyses reveal intraspecific differences	2024
2	A. Bocian; J. Buczkowicz; M. Dżugan; J. Hęclik; M. Miłek; E. Sidor	The Application of SDS-PAGE Protein and HPTLC Amino Acid Profiling for Verification of Declared Variety and Geographical Origin of Honey	2023
3	A. Bocian; M. Dżugan; P. Kielar; K. Marciniak-Lukasiak; M. Miłek; M. Mołoń; A. Pasternakiewicz ; E. Sidor	The Comparison of Honey Enriched with Laboratory Fermented Pollen vs. Natural Bee Bread in Terms of Nutritional and Antioxidant Properties, Protein In Vitro Bioaccessibility, and Its Genoprotective Effect in Yeast Cells	2023
4	A. Bocian; J. Gikunju; K. Hus; J. Kimotho ; J. Legath; E. Manson; K. Mutinda; V. Petrilla	Development of an Inhibition Enzyme-Linked Immunosorbent Assay (ELISA) Prototype for Detecting Cytotoxic Three-Finger Toxins (3FTxs) in African Spitting Cobra Venoms	2022
5	A. Bocian; K. Hus; J. Kimani; J. Kimotho ; M. Kyama; J. Legath; E. Manson; V. Petrilla	Development and Characterization of Anti-Naja ashei Three-Finger Toxins (3FTxs)-Specific Monoclonal Antibodies and Evaluation of Their In Vitro Inhibition Activity	2022
6	A. Bocian; M. Dżugan; M. Miłek; E. Sidor ; M. Tomczyk; G. Zaguła	The Use of HPTLC and SDS-PAGE Methods for Coniferous Honeydew Honey Fingerprinting Compiled with Mineral Content and Antioxidant Activity	2022
7	A. Bocian; M. Dżugan; P. Kielar; M. Miłek; E. Sidor ; K. Stępień	SDS-PAGE Protein and HPTLC Polyphenols Profiling as a Promising Tool for Authentication of Goldenrod Honey	2022
8	A. Barbasz; A. Bocian; A. Czyżowska; B. Dyba; K. Hus; J. Legath; V. Petrilla; M. Petrillova; E. Rudolphi-Szydło	Effects of 3FTx Protein Fraction from Naja ashei Venom on the Model and Native Membranes: Recognition and Implications for the Mechanisms of Toxicity	2021
9	A. Bocian; M. Dżugan; E. Kleczyńska ; M. Miłek; P. Sowa	The Comparison of Physicochemical Parameters, Antioxidant Activity and Proteins for the Raw Local Polish Honeys and Imported Honey Blends	2021
10	A. Bocian; M. Dżugan; M. Miłek; E. Sidor ; G. Zaguła	Searching for Differences in Chemical Composition and Biological Activity of Crude Drone Brood and Royal Jelly Useful for Their Authentication	2021
11	A. Bocian; J. Buczkowicz; E. Ciszkowicz; K. Hus; K. Lecka-Szlachta; J. Legath; L. Legath; V. Petrilla; M. Petrillova; M. Pietrowska	Antimicrobial Activity of Protein Fraction from Naja ashei Venom Against Staphylococcus epidermidis	2020
12	A. Bocian; J. Buczkowicz; K. Hus; M. Jaromin; J. Legath; D. Łysiak; V. Petrilla; M. Petrillova; S. Sławek	Comparison of Methods for Measuring Protein Concentration in Venom Samples	2020
13	A. Bocian; K. Hus	Antibacterial properties of snake venom components	2020
14	A. Bocian; K. Hus; E. Kuna; J. Legath; A. Lewińska; V. Petrilla; M. Petrillova; M. Wnuk	Evaluation of Antifungal Activity of Naja pallida and Naja mossambica Venoms against Three Candida Species	2020
15	A. Bocian; K. Hus; J. Legath; Ł. Marczak; V. Petrilla; M. Petrillova	Different research approaches in unraveling the venom proteome of Naja ashei	2020
16	A. Bocian; J. Buczkowicz; K. Hus; M. Jaromin; J. Legath	An effective method of isolating honey proteins	2019
17	A. Bocian; K. Hus	Dlaczego jady węży wywołują krwotoki? Krótka historia metaloproteinaz z jadów węży	2019
18	A. Bocian; K. Hus	Potencjał farmakologiczny składników jadu węży	2019
19	J. Adamczyk‐Grochala; A. Bocian; W. Hendzel; K. Hus; E. Kaleniuk; A. Lewinska; V. Petrilla; M. Petrillova; K. Szymura; M. Wnuk	Snake venoms promote stress‐induced senescence in human fibroblasts	2019