The aim of studying and bibliography

The main aim of study: To get acquainted with basic methods of in vitro cell cultivation, their application in biotechnology

The general information about the module: The module is introduced in the 2nd semester of the second degree/graduate course as faculty module for specialty. It is accomplished with 15 hours of lecture and 20 hours of laboratory work.

Bibliography required to complete the module

Bibliography used during lectures

1	Stokłosowa S.	Hodowla komórek i tkanek	PWN, Warszawa.	2004
2	Harrison M.A. Rae I.F.	General techniques of cell culture	Cambridge University Press.	1997

Bibliography used during classes/laboratories/others

1		Fundamental techniques in cell culture. A laboratory handbook	SIGMA .	2002
2	Stasiak P., Sznitowska M.,	Zastosowanie hodowli komórkowych w badaniach biofarmaceutycznych	Badania farmaceutyczne.	2010
3		MCF-7	http//www.atcc.org/ps/HTB-22.ashx.	2014
4		HEK-293	http://www.atcc.org/ps/CRL-1573.ashx.	2014

Bibliography to self-study

1

Longo-Sorbello G.S.A., Saydam G., Banerjiee D., Bertino J/R/

Cytotoxicity and Cell Growth Assays

Elsevier Science (USA) .

2006

Basic requirements in category knowledge/skills/social competences

Formal requirements: Valid registration

Basic requirements in category knowledge: Knowledge on structure and functioning of cells and methods of their study

Basic requirements in category skills: Student knows basic principles of behavior in sterile conditions

Basic requirements in category social competences: Individual work ability and team-work ability

Module outcomes

MEK	The student who completed the module	Types of classes / teaching methods leading to achieving a given outcome of teaching	Methods of verifying every mentioned outcome of teaching	Relationships with KEK	Relationships with PRK
01	To get acquainted with basic methods of in vitro cell cultivation, their application in biotechnology	lecture, laboratory	written exam	K_W03+	P7S_WG
02	Is able to conduct independent experiments with the use of selecten cell cultures	laboratory excercises	written report	K_W03+ K_U11+	P7S_UW P7S_WG
03	It has the ability to establish and passaging cells	laboratory	observation of performance	K_W03+	P7S_WG
04	Has knowledge of the use of in vitro cultures in biotechnological production	lecture, laboratory excercises	written exam	K_W03+	P7S_WG

Attention: Depending on the epidemic situation, verification of the achieved learning outcomes specified in the study program, in particular credits and examinations at the end of specific classes, can be implemented remotely (real-time meetings).

The syllabus of the module

Sem.	TK	The content	realized in	MEK
2	TK01	To get acquainted with basic methods of in vitro cell cultivation, their application in biotechnology	W02, W03, W04	MEK01 MEK04
2	TK02	Knowledge of techniques for establishing cell cultures of the cell lines, culturing and passaging cells.	L1-L4	MEK01 MEK02 MEK03
2	TK03	Knowledge of working under sterile conditions	L1-L6	MEK02
2	TK04	Knowledge of basic techniques of analysis of the selected cell lines.	L5-L6	MEK02

The student's effort

The type of classes	The work before classes	The participation in classes	The work after classes
Lecture (sem. 2)	The preparation for a test: 5.00 hours/sem.	contact hours: 15.00 hours/sem.	complementing/reading through notes: 5.00 hours/sem. Studying the recommended bibliography: 5.00 hours/sem.
Laboratory (sem. 2)	The preparation for a Laboratory: 1.00 hours/sem. The preparation for a test: 5.00 hours/sem.	contact hours: 30.00 hours/sem.	Finishing/Making the report: 2.00 hours/sem.
Advice (sem. 2)		The participation in Advice: 2.00 hours/sem.
Exam (sem. 2)	The preparation for an Exam: 10.00 hours/sem.	The written exam: 1.00 hours/sem.

The way of giving the component module grades and the final grade

The type of classes	The way of giving the final grade
Lecture	The grade is issued in a manner appropriate to the number of points received during the exam: 50-59% - [3], 60-69% - [3.5], 70-79% - [4], 80-89% - [4.5], 90-100% - [5]
Laboratory	Grade, which allows to pass the exam, is administered as a middle mark of all colloquiums
The final grade	70% Lekture + 20% Colloquiums + 10% Laboratorium

Sample problems

Required during the exam/when receiving the credit
(-)

Realized during classes/laboratories/projects
(-)

Others
(-)

Can a student use any teaching aids during the exam/when receiving the credit : no

The contents of the module are associated with the research profile: yes

1	E. Ciszkowicz; M. Dżugan; K. Lecka-Szlachta; M. Miłek; M. Tomczyk	Coniferous Honeydew Honey: Antibacterial Activity and Anti-Migration Properties against Breast Cancer Cell Line (MCF-7)	2024
2	K. Awsiuk; J. Bała; P. Chmielarz; E. Ciszkowicz; J. Raczkowska; M. Sroka; K. Wolski; I. Zaborniak	Grafting of Multifunctional Polymer Brushes from a Glass Surface: Surface-Initiated Atom Transfer Radical Polymerization as a Versatile Tool for Biomedical Materials Engineering	2024
3	Ł. Byczyński; E. Ciszkowicz; D. Czachor-Jadacka; M. Kisiel; B. Mossety-Leszczak; B. Pilch-Pitera; M. Walczak; J. Wojturska	Wodna dyspersja kationomerów uretanowo-akrylowych, sposób wytwarzania wodnej dyspersji kationomerów uretanowo-akrylowych oraz sposób wytwarzania fotoutwardzalnej powłoki z wykorzystaniem tej wodnej dyspersji	2024
4	E. Ciszkowicz; M. Dżugan; J. Hęclik; K. Lecka-Szlachta; M. Miłek; E. Sidor	The Antioxidant, Antibacterial and Anti-Biofilm Properties of Rapeseed Creamed Honey Enriched with Selected Plant Superfoods	2023
5	E. Ciszkowicz; B. Creaven; H. Jenkins; D. Kamiński; D. Karcz; K. Lecka-Szlachta; A. Matwijczuk; A. Miłoś; K. Starzak; L. Ślusarczyk	Design, Spectroscopy, and Assessment of Cholinesterase Inhibition and Antimicrobial Activities of Novel Coumarin–Thiadiazole Hybrids	2022
6	E. Ciszkowicz; M. Dżugan; K. Lecka-Szlachta; M. Miłek; A. Miłoś; A. Pasternakiewicz ; G. Zaguła	Mineral Composition, Antioxidant, Anti-Urease, and Antibiofilm Potential of Juglans Regia Leaves and Unripe Fruits	2022
7	E. Ciszkowicz; M. Dżugan; K. Lecka-Szlachta; M. Miłek; A. Pasternakiewicz ; E. Sidor ; M. Tomczyk; G. Zaguła	The Study of Chemical Profile and Antioxidant Properties of Poplar-Type Polish Propolis Considering Local Flora Diversity in Relation to Antibacterial and Anticancer Activities in Human Breast Cancer Cells	2022
8	E. Ciszkowicz; M. Kosińska-Pezda; K. Lecka-Szlachta; A. Miłoś; E. Woźnicka; L. Zapała	Analiza właściwości antybakteryjnych oraz cytotoksyczności kompleksów jonów Co(II), Mn(II) oraz Zn(II) z chryzyną oraz 3-hydroksyflawonem	2022
9	P. Chmielarz; E. Ciszkowicz; K. Lecka-Szlachta; A. Macior; J. Smenda; K. Wolski; I. Zaborniak	A New Protocol for Ash Wood Modification: Synthesis of Hydrophobic and Antibacterial Brushes from the Wood Surface	2022
10	E. Ciszkowicz; B. Creaven; H. Jenkins; D. Kamiński; D. Karcz; K. Lecka-Szlachta; A. Matwijczuk; A. Miłoś; P. Radomski; K. Starzak; L. Ślusarczyk	Novel Coumarin-Thiadiazole Hybrids and Their Cu(II) and Zn(II) Complexes as Potential Antimicrobial Agents and Acetylcholinesterase Inhibitors	2021
11	E. Ciszkowicz; K. Lecka-Szlachta; J. Pusz; E. Woźnicka	Antybakteryjna aktywność sulfonowych pochodnych chryzyny	2021
12	E. Ciszkowicz; M. Kosińska-Pezda; A. Kuźniar; K. Lecka-Szlachta; E. Pieniążek; E. Woźnicka; L. Zapała	Synteza, charakterystyka i właściwości biologiczne kompleksów jonów antanowców lekkich z 3-hydroksyflawonem	2021
13	Ł. Byczyński; E. Ciszkowicz; M. Kosińska-Pezda; K. Lecka-Szlachta; U. Maciołek; E. Woźnicka; L. Zapała; W. Zapała	Green synthesis of niflumic acid complexes with some transition metal ions (Mn(II), Fe(III), Co(II), Ni(II), Cu(II) and Zn(II)). Spectroscopic, thermoanalytical and antibacterial studies	2021
14	A. Bocian; J. Buczkowicz; E. Ciszkowicz; K. Hus; K. Lecka-Szlachta; J. Legath; L. Legath; V. Petrilla; M. Petrillova; M. Pietrowska	Antimicrobial Activity of Protein Fraction from Naja ashei Venom Against Staphylococcus epidermidis	2020
15	A. Georgiadis; N. Huleyuk; M. Kurpisz; M. Olszewska; N. Pollock; T. Stokowy; A. Yatsenko; S. Yatsenko; D. Zastavna	Familial Infertility (Azoospermia and Cryptozoospermia) in Two Brothers—Carriers of t(1;7) Complex Chromosomal Rearrangement (CCR): Molecular Cytogenetic Analysis	2020
16	E. Ciszkowicz; B. Creaven; D. Kamiński; D. Karcz; K. Lecka-Szlachta; A. Matwijczuk; K. Starzak	Structural Features of 1,3,4-Thiadiazole-Derived Ligands and Their Zn(II) and Cu(II) Complexes Which Demonstrate Synergistic Antibacterial Effects with Kanamycin	2020
17	E. Ciszkowicz; E. Kaznowska; P. Porzycki; M. Semik; M. Tyrka	MiR-93/miR-375: Diagnostic Potential, Aggressiveness Correlation and Common Target Genes in Prostate Cancer	2020
18	E. Ciszkowicz; P. Porzycki	Modern biomarkers in prostate cancer diagnosis	2020
19	E. Ciszkowicz; P. Porzycki	Detection of individual prostate cancer via multiparametric magnetic resonance imaging in own material - initial experience	2019
20	M. Fraczek; N. Huleyuk; M. Kurpisz; A. Midro; M. Olszewska; E. Wiland; D. Zastavna	Chromosome (re)positioning in spermatozoa of fathers and sons - Carriers of reciprocal chromosome translocation (RCT)	2019
21	Ł. Byczyński; M. Chutkowski; E. Ciszkowicz; M. Kosińska; K. Lecka-Szlachta; E. Woźnicka; L. Zapała; W. Zapała	Comparison of spectral and thermal properties and antibacterial activity of new binary and ternary complexes of Sm(III), Eu(III) and Gd (III) ions with N-phenylanthranilic acid and 1,10-phenanthroline	2019