The aim of studying and bibliography

The main aim of study: Provide knowledge on the modern instrumental methods, hyphenated techniques including sample preparation used in the analysis of biological material and biotechnology products.

The general information about the module: This module is taught in the first semester and involves 15 hours of lectures and laboratory: 30 hours. Module is assessed through a written exam. The module will provide students with a theoretical knowledge and experience on practical applications of advanced instrumental analysis techniques used for chemical analysis of organic and bioorganic materials.

Bibliography required to complete the module

Bibliography used during lectures

1	A. Hrynkiewicz	Fizyczne metody badań w biologii, medycynie i ochronie	Warszawa WN, Warszawa .	1999
2	A. Kozik, M. Rąpała-Kozik, I. Guevara-Lora	Analiza instrumentalna w biochemii. Wybrane problemy i metody instrumentalnej biochemii analitycznej	Instytut Biologii Molekularnej UJ, Kraków .	2001
3	R. Kocjan	Chemia analityczna. Podręcznik dla studentów. Tom 2: Analiza instrumentalna	Wydawnictwo Lekarskie PZWL, Warszawa .	2000
4	M. Szafran, Z. Dega-Szafran	Określanie struktury związków organicznych metodami spektroskopowymi	PWN, Warszawa.	1988
5	K. H. Hausser, H. R. Kalbitzer	NMR w biologii i medycynie	Wyd. Nauk. UAM, Poznań.	1993
6	R. Rosset, H. Kołodziejczyk	Współczesna chromatografia cieczowa	PWN, Warszawa.	1996
7	E. Hoffman, J. Charette, V. Stroobant	Spektrometria mas	WNT Warszawa.	1998
8	R. Krimse, J. Stach	Spektroskopia EPR Zastosowanie w chemii	Wyd. UJ Kraków.	1994
9	l. Que (Jr)	Physical Methods in bioorganic chemistry	Univ. Science Books, Sausalito, CA (USA) .	2000
10	L. Kłyszejko-Stefanowicz (red)	Ćwiczenia z biochemii	PWN Warszawa .	2003

Bibliography to self-study

1	R. A. W. Johnstone, M. E. Rose	Spektrometria mas	PWN, Warszawa.	2001
2	J. Sadlej	Spektroskopia molekularna	WNT, Warszawa.	2002
3	H. Gunther	Spektroskopia magnetycznego rezonansu jadrowego	PWN, Warszawa.	1983

Basic requirements in category knowledge/skills/social competences

Formal requirements: Required course credits: physical chemistry, analytical chemistry, instrumental analysis

Basic requirements in category knowledge: Knowledge of the physicochemical basis of spectroscopic, chromatographic and electroanalytical methods, principles of instrumentation, as well as common quantitative methods of analysis

Basic requirements in category skills: practical skills in the use of basic laboratory equipment and instrumentation

Basic requirements in category social competences: Ability to work independently and to interact in a team.

Module outcomes

MEK	The student who completed the module	Types of classes / teaching methods leading to achieving a given outcome of teaching	Methods of verifying every mentioned outcome of teaching	Relationships with KEK	Relationships with PRK
01	Mastered theoretical and practical knowledge of modern instrumental methods of analysis, including chromatography (GC &GC/GC, MCC-GC, HPLC, UHPLC), spectrometry (13C NMR, 2D NMR, MS, MS/MS), and hyphenated techniques (GC-MS, GC-MS/MS, LC-MS, LC-MS/MS) and their applications in biochemisty, drug chemistry, food scence. Acquired experience and skills iin their practical usage.	lecture, laboratory exercises	written tests and laboratory reports, completion of the lecture	K_W01++	P7S_WG
02	Has knowledge and skills in the modern techniques of preparation of samples of biological material for instrumental analysis, including purification, isolation and enrichment of analytes (SPE, SPME, HS, P&T, affinity chromatography).	lecture, laboratory exercises	written tests and laboratory reports, completion of the lecture
03	He knows the quantitative methods and calibration techniques used in analysis of samples with complex matrix. Is capable to carry out the identification and structural analysis. It able to interpret and evaluate the results of the analysis and draw conclusions.	lecture, laboratory exercises	written tests and laboratory reports, completion of the lecture	K_U11+++ K_U16+	P7S_UW
04	Is able to to select, design and perform the best analytical procedure for specific analytical problem, type of the sample and analytes.	laboratory exercises	written tests and laboratory reports	K_U08+++ K_U15++	P7S_UO P7S_UW
05	Understands the need for continuous learning and improvement of acquired skills. He can build up and update their knowledge of instruments and instrumental methods.	laboratory exercises	observation of performance	K_K01+++	P7S_KK

Attention: Depending on the epidemic situation, verification of the achieved learning outcomes specified in the study program, in particular credits and examinations at the end of specific classes, can be implemented remotely (real-time meetings).

The syllabus of the module

Sem.	TK	The content	realized in	MEK
1	TK01	Modern chromatographic techniques and separation mechanisms in quantitative and qualitative analysis of biological samples (UHPLC, GCxGC, MCC GC). Chromatography of hydrophilic interaction HILIC and Size Exclusion Ch. SEC.Methods of sample preparation, purification and isolation of analytes in different biological matrices: SPE, SPME, HS, thermal desorption.	W01-W03	MEK01
1	TK02	Advanced spectroscopic methods, including fluorescence spectroscopy and infrared and Raman spectroscopy, selected multi-pulse techniques of correlation NMR spectroscopy (COSY, TOCSY, HMQC, HSQC NOESY), EPR spectroscopy, mass spectrometry MS (ESI, APCI, APPI, MALDI, SELDI) and MS/MS and their use for the identification and structural analysis of selected classes of biologically active compounds. Bioinformatic tools for structural analysis of biomolecules.	W04-W22	MEK02
1	TK03	Coupled techniques. Variants in combining separation techniques with methods of sample preparation and spectroscopic methods of identification.	W23-W30	MEK02 MEK05
1	TK04	Determination of the active substance in the preparations and natural products - the evaluation of matrix interference effect on the accuracy and precision of quantitative analysis. Natural antioxidants in plant foods - the isolation, separation and analysis by RP-HPLC, HILC. Analysis of triglycerides in food with GC-MS - the use of mass spectrometry to determine the structure and distinguish between constitutional isomers. Isolation of methylxanthines from biological material by the solid phase extraction SPE - evaluation of the extraction yeld by HPLC. Identification of lipids and sacharides on the base of the 1 H-NMR, 13C NMR and correlation COSY, HSQC and HMBC spectra. Structural analysis of geometric and conformational isomers. The use of tandem mass spectrometry with chromatographic sepatation (LC-MS/MS) for structural and quantitative analysis of compounds.	L01-L30	MEK03 MEK04

The student's effort

The type of classes	The work before classes	The participation in classes	The work after classes
Lecture (sem. 1)		contact hours: 15.00 hours/sem.	complementing/reading through notes: 5.00 hours/sem. Studying the recommended bibliography: 10.00 hours/sem.
Laboratory (sem. 1)	The preparation for a Laboratory: 7.00 hours/sem. The preparation for a test: 14.00 hours/sem.	contact hours: 30.00 hours/sem.	Finishing/Making the report: 14.00 hours/sem.
Advice (sem. 1)	The preparation for Advice: 1.00 hours/sem.	The participation in Advice: 2.00 hours/sem.
Exam (sem. 1)	The preparation for an Exam: 15.00 hours/sem.	The written exam: 2.00 hours/sem.

The way of giving the component module grades and the final grade

The type of classes	The way of giving the final grade
Lecture	The written examination including the content of the course. The exam includes theoretical part and calculation problems. The mark (OW) depends on the score gained: 3.0 (50.0%-60.0%) MP; 3.5 (60.1%-70.0%) MP; 4.0 (70.1%-80.0%) MP; 4,5 (80.1%-90.0%) MP; 5.0 (90.1%-100%) MP. MP denotes the full score.
Laboratory	To pass the course, the student must successfully complete the laboratory experiments with positive grade. The mark obtained during each laboratory exercise is an arithmetic mean of the marks for a written/oral test, correct performance of an experiment and correct preparation of a report. The total mark in the laboratory work (OL) is calculated as arithmetic mean of the marks obtained for every exercise included in the schedule. A final mark for the laboratory is rounded according to WKJK.
The final grade	The final mark in the module (K) is calculated according to the formula: K= w 0,5 OW + w 0,5 OL; where: OW, OL denote positive marks for lecture test and laboratory practice, respectively, w- coefficient for delay, w =1.0 when a passing mark is obtained in due course, w=0.9 for a first resit, w=0.8 for a second resit. The final mark is rounded according to WKJK.

Sample problems

Required during the exam/when receiving the credit
(-)

Realized during classes/laboratories/projects
(-)

Others
(-)

Can a student use any teaching aids during the exam/when receiving the credit : no

The contents of the module are associated with the research profile: yes

1	D. Naróg; A. Sobkowiak	Electrochemistry of Flavonoids	2023
2	D. Naróg; A. Sobkowiak	Electrochemical Investigation of some Flavonoids in Aprotic Media	2022
3	D. Naróg	Electrochemical study of quercetin in the presence of galactopyranose: Potential application to the electrosynthesis of glycoconjugates of quinone/quinone methide of quercetin	2020