The aim of studying and bibliography

The main aim of study: The student will have basic informations about flow injection analysis systems, also about properties, preparations and activity of biosensors.The student know how to choose and make the right sensor for he's application and how to properly explain resived results.

The general information about the module: The module include 15 hours of lecture, and 30 hours of laboratory, and that sources of information or knowledge be appropriately credited.

Teaching materials: Instrukcje do ćwiczeń laboratoryjnych

others: Aktualne artykuły z czasopism (np. Elsevier) na temat biosensorów i analizy przepływowej

Bibliography required to complete the module

Bibliography used during lectures

1	P. Kościelniak, M. Trojanowicz	"Analiza przepływowa. Metody i zastosowania"	Wydawnictwo UJ.	2005
2	B. Karlberg, G.E. Pacey	"Wstrzykowa analiza przepływowa dla praktyków"	WNT, Warszawa .	1994
3	Z. Brzózka, W. Wróblewski	„Sensory chemiczne”	Oficyna Wydawnicza Politechniki Warszawskiej.	1988
4	Praca zbiorowa pod redakcją Z. Brzózki	"Mikrobioanalityka"	Oficyna Wydawnicza Politechniki Warszawskiej.	2009

Bibliography used during classes/laboratories/others

1	B. Karlberg, G.E. Pacey	"Wstrzykowa analiza przepływowa dla praktyków"	WNT, Warszawa , 1994.	1994
2	B.R. Eggings	" Chemical sensors and biosensors"	Willey.	2002

Bibliography to self-study

1

S. Kalinowski

"Elektrochemia membran lipidowych. Od błon komórkowych do biosensorów"

Wydawnictwo Uniwersytetu Warmińsko-Mazurskiego, Olsztyn.

2004

Basic requirements in category knowledge/skills/social competences

Formal requirements: The student have basic informations about chemical sensors, and fundamental information of instrumental analysis.

Basic requirements in category knowledge: The student know classifications and types of detectors used in chemical biosensors

Basic requirements in category skills: The student is prepared for group work, and can do the experiment by himself

Basic requirements in category social competences: The student know rules of occupational safety and health (OSH) in laboratory work and he is reliable and responsible for laboratory-based tasks.

Module outcomes

MEK	The student who completed the module	Types of classes / teaching methods leading to achieving a given outcome of teaching	Methods of verifying every mentioned outcome of teaching	Relationships with KEK	Relationships with PRK
01	The student will know basic types of biosensors, detectors used in biosensors and flow injection analysis, how to prepare of biosensors	lecture	test	K_W10++ K_K01+	P7S_KK P7S_WG
02	The student have knowledge about the applications and the actual progress of flow injection analysis and biosensors.	lecture	test	K_W10++ K_W12++	P7S_WG
03	The student know how to plan and realize the chemical experiment, can interpret results obtained for carried out exercise and can prepare a report	laboratory	test, report	K_U14+ K_K02+	P7S_KO P7S_UW
04	The student can use appropriate analytical methods to quality evaluate of biosensors and reactions catalysed by those biosensors and to choose the best available condition of reactions carried out by flow injection analysis equipment/apparatus	laboratory	test, report	K_U14+ K_K02+	P7S_KO P7S_UW
05	The student know rules of occupational safety and health (OSH) in laboratory work in particulary have protective cloth	laboratory	observation	K_W11+	P7S_WG

Attention: Depending on the epidemic situation, verification of the achieved learning outcomes specified in the study program, in particular credits and examinations at the end of specific classes, can be implemented remotely (real-time meetings).

The syllabus of the module

Sem.	TK	The content	realized in	MEK
2	TK01	Structure and principle of operation of flow systems. Theoretical basis, characteristics and parameters of flow analysis. Kinds of flow analysis techniques. Dispersion in the FIA (Flow Injection Analysis). Detection in the FIA. Applications of Flow Injection Methods in Routine Analysis. Commercially Available Instrumentation for FIA. Current Trends in Developments of Flow Analysis. Introduction to Biosensors, types of biosensors. Biosensors using biocatalysts. Theoretical basis of enzymatic sensors. Enzyme and protein immobilization methods. Biocatalytic materials in biosensors. Biosensors using receptors, nucleic acid and antibodies. Other biological receptor materials. Immunochemical selectivity. Detection systems in biosensors – electrochemical, mass, thermal and optical sensors. Theoretical aspects of transducer operation contacting with biological recognition layer – examples of practical solutions. Application of biosensors in clinical chemistry and environmental protection. Prospects for the development of flow analysis sensors and biosensors.	W1-W15	MEK01 MEK02 MEK04
2	TK02	Determination of flavonoids in beer by means of enzyme biosensors utilizing plant tissue. Determination of glucose by biosensor. Biosensor based on immobilized laccase on surface of glassy carbon electrode - properties and principle of operation. Detection of o-phenols by the use of biosensor containing immobilized enzyme - tyrosinase. Application of ion-selective electrodes for environmental determinations. Detection of base by the use FIA technique. Spectrophotometric determination of Iron with the use of Flow Techniques. Electrochemical Detection of Iron using FIA systems.	L1-L30	MEK02 MEK03 MEK04 MEK05

The student's effort

The type of classes	The work before classes	The participation in classes	The work after classes
Lecture (sem. 2)		contact hours: 15.00 hours/sem.	complementing/reading through notes: 5.00 hours/sem. Studying the recommended bibliography: 1.50 hours/sem.
Laboratory (sem. 2)	The preparation for a Laboratory: 1.00 hours/sem. The preparation for a test: 10.00 hours/sem.	contact hours: 30.00 hours/sem.	Finishing/Making the report: 2.50 hours/sem.
Advice (sem. 2)		The participation in Advice: 2.00 hours/sem.
Credit (sem. 2)	The preparation for a Credit: 18.00 hours/sem.	The written credit: 1.50 hours/sem. The oral credit: 0.50 hours/sem.

The way of giving the component module grades and the final grade

The type of classes	The way of giving the final grade
Lecture	Lecture: written test Written credit of lecture covering the entire range of material of lecture. Evaluation of the test depends on the number of points scored: 3,0 (50,0 %-60,0%) MP ; 3,5 (60,1%-70,0%) MP; 4,0 (70,1%-80,0%) MP; 4,5 (80,1%-90,0%) MP; 5,0 (90,1%-100%) MP. MP- max. points.
Laboratory	Laboratory: written tests, laboratory reports Evaluation of the test depends on the number of points scored: 3,0 (50,0 %-60,0%) MP ; 3,5 (60,1%-70,0%) MP; 4,0 (70,1%-80,0%) MP; 4,5 (80,1%-90,0%) MP; 5,0 (90,1%-100%) MP. MP- max. points.
The final grade	Final grade (K): K = 0,50 * W * w + 0,50 * L * w; where: W, L - positive evaluation of the credit of lecture, laboratory; w - factor related to the time of credit or laboratory, w= 1.0 first term, w = 0.9 second term , w = 0.8 third term.The grade is rounded according to WKZJK.

Sample problems

Required during the exam/when receiving the credit
(-)

Realized during classes/laboratories/projects
(-)

Others
(-)

Can a student use any teaching aids during the exam/when receiving the credit : no

The contents of the module are associated with the research profile: yes

1	K. Rydel-Ciszek	DFT Studies of the Activity and Reactivity of Limonene in Comparison with Selected Monoterpenes	2024
2	P. Chmielarz; T. Pacześniak; K. Rydel-Ciszek; A. Sobkowiak	Bio-Inspired Iron Pentadentate Complexes as Dioxygen Activators in the Oxidation of Cyclohexene and Limonene	2023
3	K. Rydel-Ciszek	The most reactive iron and manganese complexes with N-pentadentate ligands for dioxygen activation—synthesis, characteristics, applications	2021
4	P. Chmielarz; A. Miłaczewska; T. Pacześniak; K. Rydel-Ciszek; A. Sobkowiak	‘Oxygen-Consuming Complexes’–Catalytic Effects of Iron–Salen Complexes with Dioxygen	2021
5	P. Błoniarz; P. Chmielarz; T. Pacześniak; K. Rydel-Ciszek; A. Sobkowiak; K. Surmacz; I. Zaborniak	Iron-Based Catalytically Active Complexes in Preparation of Functional Materials	2020