The aim of studying and bibliography

The main aim of study: To get acquainted with the structure and properties of proteins with particular emphasis on proteins used in medicine.

The general information about the module: The module is introduced in the 5th semester of the first degree/ undergraduate course. It is accomplished with 15 hours of lecture and 15 hours of laboratories. It ends with an examination-based assessment.

Bibliography required to complete the module

Bibliography used during lectures

1	Agnieszka Kraj, Anna Drabik, Jerzy Silberring	Proteomika i metabolomika	WUW.	2010
2	P.C. Turner et al.	Krótkie wykłady: Biologia molekularna	Wydawnictwo Naukowe PWN.	2001
3	B.D. Hames et al.	Krótkie wykłady: Biochemia	Wydawnictwo naukowe PWN.	2002
4	Jeremy M. Berg, Lubert Stryer, John L. Tymoczko	Biochemia	Wydawnictwo Naukowe PWN.	2011

Basic requirements in category knowledge/skills/social competences

Formal requirements: Valid registration

Basic requirements in category knowledge: Basic knowledge of biochemistry

Basic requirements in category skills: Self-education skills, ability to operate basic laboratory equipment

Basic requirements in category social competences: Ability to work both alone and in a group

Module outcomes

MEK	The student who completed the module	Types of classes / teaching methods leading to achieving a given outcome of teaching	Methods of verifying every mentioned outcome of teaching	Relationships with KEK	Relationships with PRK
01	He knows the structure and functions of proteins.	lecture	written test	K_W02+++ K_W06++	P6S_WG
02	Knows the process of protein translation and the basics of post-translational modifications	lecture	written test	K_W06+++	P6S_WG
03	Knows the techniques used to separate, identify and characterise proteins.	lecture, laboratory	written tests	K_W07+++ K_U05+++ K_K03+	P6S_KR P6S_UW P6S_WG
04	Understands the structure and function of antibodies as well as other components of the immune system (cells, proteins and peptides)	lecture	written test	K_W07+	P6S_WG

Attention: Depending on the epidemic situation, verification of the achieved learning outcomes specified in the study program, in particular credits and examinations at the end of specific classes, can be implemented remotely (real-time meetings).

The syllabus of the module

Sem.	TK	The content	realized in	MEK
5	TK01	Structure and functions of proteins	W1-W7	MEK01
5	TK02	Techniques used to protein research	L1-5, W1-7	MEK03
5	TK03	Structure of antibodies and basics of immunology	W1-7	MEK04
5	TK04	Translation and post-translational modifications	W1-W7	MEK02

The student's effort

The type of classes	The work before classes	The participation in classes	The work after classes
Lecture (sem. 5)		contact hours: 15.00 hours/sem.	complementing/reading through notes: 2.00 hours/sem.
Laboratory (sem. 5)	The preparation for a Laboratory: 5.00 hours/sem. The preparation for a test: 5.00 hours/sem.	contact hours: 15.00 hours/sem.	Finishing/Making the report: 5.00 hours/sem.
Advice (sem. 5)
Credit (sem. 5)	The preparation for a Credit: 5.00 hours/sem.	The written credit: 2.00 hours/sem.

The way of giving the component module grades and the final grade

The type of classes	The way of giving the final grade
Lecture	The note is proportional to the number of points earned during the test and follows criteria of the 'Wydziałowa Księga Jakości Kształcenia'. The positive note is achieved only when 50% of points are collected.
Laboratory	The note is based on the mean score of the tests and the evaluation of the report.
The final grade	The final grade is the average of the grade of lectures and a final evaluation exercise (50/50). The average may be drawn only if both grades are positive

Sample problems

Required during the exam/when receiving the credit
(-)

Realized during classes/laboratories/projects
(-)

Others
(-)

Can a student use any teaching aids during the exam/when receiving the credit : no

The contents of the module are associated with the research profile: yes

1	A. Bocian; J. Buczkowicz; K. Hus; J. Legath; T. Litschka-Koen; V. Petrilla; M. Petrillova; M. Pietrowska	Venom diversity in Naja mossambica: Insights from proteomic and immunochemical analyses reveal intraspecific differences	2024
2	A. Bocian; J. Buczkowicz; M. Dżugan; J. Hęclik; M. Miłek; E. Sidor	The Application of SDS-PAGE Protein and HPTLC Amino Acid Profiling for Verification of Declared Variety and Geographical Origin of Honey	2023
3	A. Bocian; M. Dżugan; P. Kielar; K. Marciniak-Lukasiak; M. Miłek; M. Mołoń; A. Pasternakiewicz ; E. Sidor	The Comparison of Honey Enriched with Laboratory Fermented Pollen vs. Natural Bee Bread in Terms of Nutritional and Antioxidant Properties, Protein In Vitro Bioaccessibility, and Its Genoprotective Effect in Yeast Cells	2023
4	A. Bocian; J. Gikunju; K. Hus; J. Kimotho ; J. Legath; E. Manson; K. Mutinda; V. Petrilla	Development of an Inhibition Enzyme-Linked Immunosorbent Assay (ELISA) Prototype for Detecting Cytotoxic Three-Finger Toxins (3FTxs) in African Spitting Cobra Venoms	2022
5	A. Bocian; K. Hus; J. Kimani; J. Kimotho ; M. Kyama; J. Legath; E. Manson; V. Petrilla	Development and Characterization of Anti-Naja ashei Three-Finger Toxins (3FTxs)-Specific Monoclonal Antibodies and Evaluation of Their In Vitro Inhibition Activity	2022
6	A. Bocian; M. Dżugan; M. Miłek; E. Sidor ; M. Tomczyk; G. Zaguła	The Use of HPTLC and SDS-PAGE Methods for Coniferous Honeydew Honey Fingerprinting Compiled with Mineral Content and Antioxidant Activity	2022
7	A. Bocian; M. Dżugan; P. Kielar; M. Miłek; E. Sidor ; K. Stępień	SDS-PAGE Protein and HPTLC Polyphenols Profiling as a Promising Tool for Authentication of Goldenrod Honey	2022
8	A. Barbasz; A. Bocian; A. Czyżowska; B. Dyba; K. Hus; J. Legath; V. Petrilla; M. Petrillova; E. Rudolphi-Szydło	Effects of 3FTx Protein Fraction from Naja ashei Venom on the Model and Native Membranes: Recognition and Implications for the Mechanisms of Toxicity	2021
9	A. Bocian; M. Dżugan; E. Kleczyńska ; M. Miłek; P. Sowa	The Comparison of Physicochemical Parameters, Antioxidant Activity and Proteins for the Raw Local Polish Honeys and Imported Honey Blends	2021
10	A. Bocian; M. Dżugan; M. Miłek; E. Sidor ; G. Zaguła	Searching for Differences in Chemical Composition and Biological Activity of Crude Drone Brood and Royal Jelly Useful for Their Authentication	2021
11	A. Bocian; J. Buczkowicz; E. Ciszkowicz; K. Hus; K. Lecka-Szlachta; J. Legath; L. Legath; V. Petrilla; M. Petrillova; M. Pietrowska	Antimicrobial Activity of Protein Fraction from Naja ashei Venom Against Staphylococcus epidermidis	2020
12	A. Bocian; J. Buczkowicz; K. Hus; M. Jaromin; J. Legath; D. Łysiak; V. Petrilla; M. Petrillova; S. Sławek	Comparison of Methods for Measuring Protein Concentration in Venom Samples	2020
13	A. Bocian; K. Hus	Antibacterial properties of snake venom components	2020
14	A. Bocian; K. Hus; E. Kuna; J. Legath; A. Lewińska; V. Petrilla; M. Petrillova; M. Wnuk	Evaluation of Antifungal Activity of Naja pallida and Naja mossambica Venoms against Three Candida Species	2020
15	A. Bocian; K. Hus; J. Legath; Ł. Marczak; V. Petrilla; M. Petrillova	Different research approaches in unraveling the venom proteome of Naja ashei	2020
16	A. Bocian; J. Buczkowicz; K. Hus; M. Jaromin; J. Legath	An effective method of isolating honey proteins	2019
17	A. Bocian; K. Hus	Dlaczego jady węży wywołują krwotoki? Krótka historia metaloproteinaz z jadów węży	2019
18	A. Bocian; K. Hus	Potencjał farmakologiczny składników jadu węży	2019
19	J. Adamczyk‐Grochala; A. Bocian; W. Hendzel; K. Hus; E. Kaleniuk; A. Lewinska; V. Petrilla; M. Petrillova; K. Szymura; M. Wnuk	Snake venoms promote stress‐induced senescence in human fibroblasts	2019