The aim of studying and bibliography

The main aim of study: To provide students with the basic methods of instrumental analysis, construction and operation of the equipment and its use for the determination of the composition, purity of biotechnology products and materials.

The general information about the module: The module is implemented in the 5th semester and includes 30 hours of lecture and 45 hours of laboratory. The module ends with an exam

others: instrukcje do ćwiczeń laboratoryjnych

Bibliography required to complete the module

Bibliography used during lectures

1	A.Cygański	Metody spektroskopowe w chemii analitycznej	WNT Warszawa.	1997
2	E. Hoffmann, J. Charette, V. Stroobant	Spektrometria mas	WNT Warszawa.	1998
3	A. Cygański	Metody elektroanalityczne	WNT Warszawa.	1995
4	J. krysiak	Chemiczna analiza instrumentalna	PZWL.	1999
5	Z. Witkiewicz	Podstawy chromatografii	WNT Warszawa.	2005

Bibliography used during classes/laboratories/others

1	W. Szczepaniak	Metody instrumentalne w analizie chemicznej	PWN Warszawa.	2004
2	R.M. Silverstein i inn.	Spekroskopowe metody identyfikacji związkow organicznych	PWN Warszawa.	2007

Bibliography to self-study

1

W. Szczepaniak

Metody instrumentalne w analizie chemicznej

PWN Warszawa.

2004

Basic requirements in category knowledge/skills/social competences

Formal requirements: completed course of general chemistry, analytical chemistry,and physical chemistry

Basic requirements in category knowledge: Knowledge of physics, analytical, organic and physical chemistry.

Basic requirements in category skills: Basic skills relating to the calculation of analyte concentration, preparing solutions and the use of the chemical laboratory equipment.

Basic requirements in category social competences: Knowledge of safety rules in the chemical laboratory, the responsibility required during chemical experiments performed individually and in a group.

Module outcomes

MEK	The student who completed the module	Types of classes / teaching methods leading to achieving a given outcome of teaching	Methods of verifying every mentioned outcome of teaching	Relationships with KEK	Relationships with PRK
01	Has knowledge about theoretical foundations of the common intrumental methods of analysis, principles of operation and construction of modern analytical instrumentation.	lecture, laboratory	written test and report for every laboratory exercise, observation of performance,	K_W02+ K_W08++ K_U06+ K_U15+	P6S_UU P6S_UW P6S_WG
02	Acquired knowlewdge on the application of the various instrumental techniques to the solution of analytical problems in experimental research, biotechnology and chemical production.	lecture	pass a final written exam	K_W07+ K_W08++ K_U06++	P6S_UW P6S_WG
03	Able to select and justify the use of instrumental methods of analysis in terms of analytical and economic requirements.	lecture,	written test and report for every laboratory exercise, observation of performance,	K_U01+ K_U06++ K_K03+	P6S_KR P6S_UK P6S_UW
04	Mastered the practical usage of the instrumental methods to perform chemical analyses	laboratory	written test , observation of performance,	K_U05+ K_U06++ K_K02+	P6S_KO P6S_KR P6S_UW
05	Knows and can practically use the qualitative and quantitative methods of analysis, and calibration techniques.	lecture, laboratory	written test , observation of performance,	K_U06+++	P6S_UW
06	Knows how to prepare a report on laboratory exercises, is able to evaluate the results and draw valid conclusions.	laboratory	written raport	K_U01+	P6S_UK P6S_UW
07	Has ability to work independently and to interact in a team.	laboratory	observation of performance,	K_U14++ K_K02++ K_K03++	P6S_KO P6S_KR P6S_UO
08	Knows the principles of good laboratory practice, including the principles of safety, waste collection.	laboratory	observation of performance,	K_K02++	P6S_KO P6S_KR
09	Understands the need for continuous complement of knowledge about the new solutions and methods of instrumental analysis.	lecture, laboratory	observation of performance,	K_K01++	P6S_KK

Attention: Depending on the epidemic situation, verification of the achieved learning outcomes specified in the study program, in particular credits and examinations at the end of specific classes, can be implemented remotely (real-time meetings).

The syllabus of the module

Sem.	TK	The content	realized in	MEK
5	TK01	Analytical process, its elements and statistical evaluation of each step. Analysis of elements and compounds by spectroscopic methods. Atomic Emission Spectroscopy - basis of the method, methods of sample atomization and excitation, applications. Atomic absorption spectroscopy. Molecular spectroscopy in the ultraviolet and visible light. Infrared spectroscopy. Spectra recording techniques, methods of quantitative and qualitative analysis. Fundamentals of nuclear magnetic resonance spectroscopy. The quantitative and structural analysis based on the NMR spectra. Fundamentals of mass spectrometry. Interpretation and application of analytical mass spectra for organic compounds. Chromatographic methods for separation of mixtures. Basic principles and classification. Theoreticasl basis of separation process. Retention mechanisms and parameters. Separation efficiency. Definition and determination of resolution index, theoretical plate number, selectivity factor. Separation techniques in liquid chromatography - adsorption chromatography, partition - normal/reverse chromatography, ion-exchange chromatography , gel filtration chromatography. Selection of the chromatographic conditions - rules for the selection of the stationary and mobile phases. High Performance Liquid Chromatography HPLC and thin-layer HPTLC. Isocratic and gradient techniques, instrumentation. Gas chromatography. The rate theory of chromatography - band broadening, column efficiency. Optimization of column performance. Chromatographic methods of qualitative and quantitative analysis. Potentiometric methods. Design, operation and application of the selected ion-selective electrodes. Conductometry and its analytical application. Voltammetric methods - linear-sweep LSV, cyclic CV, and stripplng CSV, ASV techniques. Quantitative and qualitative analysis. Selected applications in analytical laboratory and industrial applications, criteria for the method selection.	W30	MEK01 MEK03 MEK05 MEK09
5	TK02	Gas Chromatography. Identification of components in a mixture of hydrocarbons. Quantitative determination of the substance content in a multi-component mixture. Liquid chromatography. Determination of caffeine using liquid chromatography. Mass spectrometry. Analysis of the composition of the mixture of hydrocarbons using gas chromatography coupled with mass spectrometry. Analysis of the structure of organic compounds by IR spectroscopy. Basic rules for the interpretation of IR spectra. Methods of sample preparation in IR spectroscopy. Determination of the parameters of the absorption band and the molar absorption coefficient. Quantitative determination of the content of picric acid in the test sample. Analysis of 1H-NMR spectra. Determination of the content of elements in solutions by atomic absorption spectroscopy (AAS). Polarimetric determination of sucrose concentration in aqueous solutions. Quantitative determination of paracetamol content by cyclic voltammetry. Determination of the concentration of iodides and chlorides side by side by potentiometric precipitation titration. Determination of weak acid concentration by conductometric titration.	L45	MEK01 MEK02 MEK03 MEK04 MEK05 MEK06 MEK07 MEK08

The student's effort

The type of classes	The work before classes	The participation in classes	The work after classes
Lecture (sem. 5)	The preparation for a test: 3.00 hours/sem.	contact hours: 30.00 hours/sem.	complementing/reading through notes: 15.00 hours/sem. Studying the recommended bibliography: 15.00 hours/sem.
Laboratory (sem. 5)	The preparation for a Laboratory: 5.00 hours/sem. The preparation for a test: 20.00 hours/sem.	contact hours: 45.00 hours/sem.	Finishing/Making the report: 20.00 hours/sem.
Advice (sem. 5)		The participation in Advice: 2.00 hours/sem.
Exam (sem. 5)	The preparation for an Exam: 20.00 hours/sem.	The written exam: 2.00 hours/sem.

The way of giving the component module grades and the final grade

The type of classes	The way of giving the final grade
Lecture	Written examination in the form of a test (51% of the maximum number of points is required to pass) -OE. The test includes theoretical part and calculation problems. The mark (OE) depends on the score gained: 3.0 (50.0%-60.0%) MP ; 3.5 (60.1%-70.0%) MP; 4.0 (70.1%-80.0%) MP; 4,5 (80.1%-90.0%) MP; 5.0 (90.1%-100%) MP. MP denotes the full score.
Laboratory	The necessary condition for receiving a credit for the laboratory is obtaining at least a passing mark on each exercise included in the schedule. The mark obtained during each laboratory exercise is an arithmetic mean of the marks for a written/oral test, correct performance of an experiment and correct preparation of a report. The total mark in the laboratory work (OL) is calculated as arithmetic mean of the marks obtained for every exercise included in the schedule.
The final grade	The final mark in the module (K) is calculated according to the formula: K= w 0,5 OE + w 0,5 OL; where: OE, OL denote positive marks for lecture test and laboratory practice, respectively, w - coefficient for delay, w =1.0 when a passing mark is obtained in due time, w=0.9 for a first resit, w=0.8 for a second resit. The final mark is rounded according to WKZJK.

Sample problems

Required during the exam/when receiving the credit
(-)

Realized during classes/laboratories/projects
(-)

Others
(-)

Can a student use any teaching aids during the exam/when receiving the credit : no

The contents of the module are associated with the research profile: yes

1	D. Naróg; A. Sobkowiak	Electrochemistry of Flavonoids	2023
2	D. Naróg; A. Sobkowiak	Electrochemical Investigation of some Flavonoids in Aprotic Media	2022
3	D. Naróg	Electrochemical study of quercetin in the presence of galactopyranose: Potential application to the electrosynthesis of glycoconjugates of quinone/quinone methide of quercetin	2020