Cycle of education: 2022/2023
The name of the faculty organization unit: The faculty Chemistry
The name of the field of study: Biotechnology
The area of study: technical sciences
The profile of studing:
The level of study: first degree study
Type of study: past time
discipline specialities : Applied biochemistry, Purification and analysis of biotechnological products
The degree after graduating from university: Bachelor of Science (BSc)
The name of the module department : Department of Polymers and Biopolymers
The code of the module: 10992
The module status: mandatory for the speciality Applied biochemistry
The position in the studies teaching programme: sem: 5 / W9 L9 / 3 ECTS / E
The language of the lecture: Polish
The name of the coordinator: Prof. Tomasz Ruman, DSc, PhD, Eng.
The main aim of study: Introduction of basics of chemical analysis. Modern and classical methods of biomolecules isolation, purification and analysis. Basic knowledge of proteomics is also discussed. New methods of protein post-translational modifications determination. Application of NMR, FTIR, MS, CD, XRD, fluorescence and other methods etc along with various purification techniques (electrophoresis, chromatography etc.). Methods dedicated for small molecules (for example metabolites) and biomacromolecules.
The general information about the module: Module is realized in 5th semester. Contain 15hours of lectures and 15 hours of laboratory classes. Module ends with written colloquium.
Teaching materials: tr.sd.prz.edu.pl
1 | Robert M. Silverstein, Francis X. Webster, David J. Kiemle | Spektroskopowe metody identyfikacji związków organicznych | Wydawnictwo Naukowe PWN. | 2012 |
2 | W. Zielinski, A. Rajca | Metody spektroskopowe i ich zastosowanie do identyfikacji zwiazków organicznych | Wydawnictwo Naukowo Techniczne. | 1995 |
3 | M. Szafran, Z. Dega-Szafran | Okreslanie struktury zwiazków organicznych metodami spektroskopowymi | Wydawnictwo Naukowe PWN. | 1988 |
4 | L.A. Kazicyna, N.B. Kupletska | Metody spektroskopowe wyznaczania struktury zwiazków organicznych | Wydawnictwo Naukowe PWN. | 1974 |
5 | Walczyna, J. Sokołowski, G. Kupryszewski | Analiza zwiazków organicznych | Wydawnictwo Uniwersytetu Gdańskiego. | 1996 |
6 | L. Kłyszejko-Stefanowicz | Ćwiczenia z biochemii | Wydawnictwo Naukowe PWN. | 2005 |
7 | A. Berthillier | Chromatografia i jej zastosowanie | Wydawnictwo Naukowe PWN. | 1975 |
8 | A. Kraj, J. Silberring | Proteomika | Wydział Chemii UJ. | 2004 |
9 | A. Dubin | Wprowadzenie do chemii białek | Wydział Biotechnologii UJ. | 2003 |
10 | Walkowiak B. Techniki chromatografii cieczowej. Przykłady zastosowań. | Techniki chromatografii cieczowej. Przykłady zastosowań | Amersham Pharmacia Biotech. MORPOL. | 2000 |
11 | Kozik A., Rąpała-Kozik M., Guevara-Lora I | Analiza instrumentalna w biochemii. Wybrane problemy i metody instrumentalnej biochemii analitycznej | Instytutu Biologii Molekularnej UJ. | 2001 |
12 | A. Kraj, A. Drabik, J.Silberring | Proteomika i metabolomika | Wydawnictwo Uniwersytetu Warszawskiego. | 2010 |
Formal requirements: semester registration
Basic requirements in category knowledge: Composition of proteins and nucleic acids. Composition od function of saccharides, amino acids, peptides and other low molecular weight organics of biological origin.
Basic requirements in category skills: Good technical or chemical english; able of internet and literature search; laboratory practice with corrosive compounds and automatic pipettes.
Basic requirements in category social competences: effective collaboration with team members
MEK | The student who completed the module | Types of classes / teaching methods leading to achieving a given outcome of teaching | Methods of verifying every mentioned outcome of teaching | Relationships with KEK | Relationships with PRK |
---|---|---|---|---|---|
01 | know physical background of methods: NMR, FTIR, MS | lectures | written exam |
K_W04+ K_W05+ K_W10+ K_U03+ |
P6S_UK P6S_WG |
02 | can work with biomolecules without its decomposition; can perform analyses and results interpretation | Lectures | colloquium |
K_W10+ K_U03+ K_U09+++ K_K03+ |
P6S_KR P6S_UK P6S_UO P6S_UW P6S_WG |
Attention: Depending on the epidemic situation, verification of the achieved learning outcomes specified in the study program, in particular credits and examinations at the end of specific classes, can be implemented remotely (real-time meetings).
Sem. | TK | The content | realized in | MEK |
---|---|---|---|---|
5 | TK01 | W01-W05 | MEK01 MEK02 | |
5 | TK02 | W06-W10 | MEK02 | |
5 | TK03 | W11-15 | MEK02 |
The type of classes | The work before classes | The participation in classes | The work after classes |
---|---|---|---|
Lecture (sem. 5) | The preparation for a test:
9.00 hours/sem. |
contact hours:
9.00 hours/sem. |
complementing/reading through notes:
5.00 hours/sem. Studying the recommended bibliography: 15.00 hours/sem. |
Laboratory (sem. 5) | The preparation for a Laboratory:
5.00 hours/sem. The preparation for a test: 15.00 hours/sem. |
contact hours:
9.00 hours/sem. |
Finishing/Making the report:
5.00 hours/sem. |
Advice (sem. 5) | |||
Exam (sem. 5) | The preparation for an Exam:
15.00 hours/sem. |
The written exam:
2.00 hours/sem. |
The type of classes | The way of giving the final grade |
---|---|
Lecture | exam |
Laboratory | colloquium |
The final grade | Final grade K = 0,5wW+ 0,5wL, where W - lecture grade (lecture material colloquium/exam grade) L - average of laboratory colloquia grades w - factor of positive grade session number - 1 for first, 0.9 for second, 0.8 for third Positive grade - min 50% of maximum points; every 10% more - grade rises of 0.5 up to 5.0 |
Required during the exam/when receiving the credit
(-)
Realized during classes/laboratories/projects
(-)
Others
(-)
Can a student use any teaching aids during the exam/when receiving the credit : no
1 | A. Kołodziej; Z. Krupa; J. Nizioł; A. Ossolińska; K. Ossoliński; T. Ossoliński; A. Płaza-Altamer; T. Ruman | Untargeted metabolomics of bladder tissue using liquid chromatography and quadrupole time-of-flight mass spectrometry for cancer biomarker detection | 2024 |
2 | B. Guratowska; A. Kuźniar; J. Nizioł; A. Nowak; M. Okrasa; T. Ruman; M. Ryngajłło; J. Szulc | Uncontrolled Post-Industrial Landfill—Source of Metals, Potential Toxic Compounds, Dust, and Pathogens in Environment—A Case Study | 2024 |
3 | V. Copie; A. Kołodziej; Z. Krupa; J. Nizioł; A. Ossolińska; K. Ossoliński; T. Ossoliński; A. Płaza-Altamer; T. Ruman; B. Tripet | Metabolomic profiling of human bladder tissue extracts | 2024 |
4 | Z. Krupa; M. Misiorek; J. Nizioł; T. Ruman | Infrared Laser-Based Selected Reaction Monitoring Mass Spectrometry Imaging of Banana (Musa spp.) Tissue—New Method for Detection and Spatial Localization of Metabolites in Food | 2024 |
5 | A. Arendowski; A. Kołodziej; J. Nizioł; A. Ossolińska; K. Ossoliński; T. Ossoliński; A. Płaza-Altamer; T. Ruman | Monoisotopic silver nanoparticles-based mass spectrometry imaging of human bladder cancer tissue: Biomarker discovery | 2023 |
6 | A. Kołodziej; A. Nieczaj; J. Nizioł; A. Ossolińska; K. Ossoliński; T. Ossoliński; A. Płaza-Altamer; T. Ruman | Untargeted urinary metabolomics for bladder cancer biomarker screening with ultrahigh-resolution mass spectrometry | 2023 |
7 | A. Kołodziej; Z. Krupa; J. Nizioł; A. Płaza-Altamer; T. Ruman | Infrared pulsed fiber laser-produced gold and silver-109 nanoparticles for laser desorption/ionization mass spectrometry of steroid hormones | 2023 |
8 | M. Dudek; B. Gutarowska; M. Komar; J. Nizioł; P. Nowicka-Krawczyk; T. Ruman | Biodeterioration potential of algae on building materials - Model study | 2023 |
9 | S. Kuberski; A. Kuźniar; J. Nizioł; A. Nowak; I. Nowak; M. Okrasa; T. Ruman; B. Szponar; J. Szulc | Biological and chemical contamination of illegal, uncontrolled refuse storage areas in Poland | 2023 |
10 | V. Copie; A. Kołodziej; A. Nieczaj; J. Nizioł; A. Ossolińska; K. Ossoliński; T. Ossoliński; A. Płaza-Altamer; T. Ruman; B. Tripet | Targeted and untargeted urinary metabolic profiling of bladder cancer | 2023 |
11 | A. Kołodziej; J. Nizioł; A. Ossolińska; K. Ossoliński; T. Ossoliński; A. Płaza-Altamer; T. Ruman | Untargeted ultra-high-resolution mass spectrometry metabolomic profiling of blood serum in bladder cancer | 2022 |
12 | A. Kołodziej; J. Nizioł; A. Płaza-Altamer; T. Ruman | Infrared pulsed fiber laser-produced silver-109 nanoparticles for laser desorption/ionization mass spectrometry of 3-hydroxycarboxylic acids | 2022 |
13 | A. Kołodziej; J. Nizioł; A. Płaza-Altamer; T. Ruman | Infrared pulsed fiber laser-produced silver-109-nanoparticles for laser desorption/ionization mass spectrometry of amino acids | 2022 |
14 | A. Kołodziej; J. Nizioł; A. Płaza-Altamer; T. Ruman | Infrared pulsed fiber laser-produced silver-109-nanoparticles for laser desorption/ionization mass spectrometry of carboxylic acids | 2022 |
15 | A. Kołodziej; J. Nizioł; A. Płaza-Altamer; T. Ruman | Laser Ablation Synthesis in Solution and Nebulization of Silver-109 Nanoparticles for Mass Spectrometry and Mass Spectrometry Imaging | 2022 |
16 | A. Kołodziej; J. Nizioł; A. Płaza-Altamer; T. Ruman | Laser generated gold nanoparticles for mass spectrometry of low molecular weight compounds | 2022 |
17 | A. Kołodziej; J. Nizioł; A. Płaza-Altamer; T. Ruman | Obrazowanie tkanek za pomocą spektrometrii mas z laserową desorpcją/jonizacją | 2022 |
18 | B. Gutarowska; M. Komar; P. Konca; J. Nizioł; P. Nowicka-Krawczyk; T. Ruman | Metabolomic analysis of photosynthetic biofilms on building façades in temperate climate zones | 2022 |
19 | B. Gutarowska; T. Ruman; J. Szulc | Metagenomika i metabolomika – nowoczesne metody systemowe w identyfikacji mikroorganizmów oraz metabolitów odpowiedzialnych za niszczenie obiektów zabytkowych | 2022 |
20 | S. Kuberski; J. Nizioł; A. Nowak; M. Okrasa; T. Ruman; J. Szulc | Assessment of Physicochemical, Microbiological and Toxicological Hazards at an Illegal Landfill in Central Poland | 2022 |
21 | V. Copie; A. Kołodziej; J. Nizioł; K. Nogueira; L. Nogueira; A. Ossolińska; K. Ossoliński; T. Ossoliński; A. Płaza-Altamer; T. Ruman; B. Tripet | Metabolomic and elemental profiling of blood serum in bladder cancer | 2022 |
22 | A. Arendowski; J. Nizioł; A. Ossolińska; K. Ossoliński; T. Ossoliński; T. Ruman | Serum and urine analysis with gold nanoparticle-assisted laser desorption/ionization mass spectrometry for renal cell carcinoma metabolic biomarkers discovery | 2021 |
23 | A. Arendowski; V. Copie; J. Nizioł; K. Nogueira; L. Nogueira; K. Ossoliński; T. Ruman; B. Tripet | Metabolomic and elemental profiling of human tissue in kidney cancer | 2021 |
24 | A. Arendowski; V. Copie; J. Nizioł; K. Ossoliński; T. Ruman; B. Tripet | Nuclear magnetic resonance and surface-assisted laser desorption/ionization mass spectrometry-based metabolome profiling of urine samples from kidney cancer patients | 2021 |
25 | A. Kołodziej; T. Ruman; J. Szulc | Silver-109/Silver/Gold Nanoparticle-Enhanced Target Surface-Assisted Laser Desorption/Ionisation Mass Spectrometry—The New Methods for an Assessment of Mycotoxin Concentration on Building Materials | 2021 |
26 | B. Gutarowska; K. Majchrzycka; J. Nizioł; A. Nowak; M. Okrasa; T. Ruman; M. Sulyok; B. Szponar; J. Szulc | Microbiological and Toxicological Hazards in Sewage Treatment Plant Bioaerosol and Dust | 2021 |
27 | I. Beech; A. Drążkowska; B. Guratowska; J. Karbowska-Berent; T. Ruman; J. Sunner; J. Szulc | Metabolomics and metagenomics analysis of 18th century archaeological silk | 2021 |
28 | M. Misiorek; J. Nizioł; T. Ruman | Zastosowanie spektometrii mas do obrazowania rozmieszczenia flawonoidów w owocu truskawki | 2021 |
29 | A. Arendowski; J. Nizioł; K. Ossoliński; T. Ruman | Gold nanostructures - assisted laser desorption/ionization mass spectrometry for kidney cancer blood serum biomarker screening | 2020 |
30 | A. Arendowski; J. Nizioł; K. Ossoliński; T. Ruman | Screening of Urinary Renal Cancer Metabolic Biomarkers with Gold Nanoparticles-assisted Laser Desorption/Ionization Mass Spectrometry | 2020 |
31 | A. Arendowski; V. Copie; J. Nizioł; K. Ossoliński; T. Ruman; B. Tripet | Nuclear magnetic resonance and surface-assisted laser desorption/ionization mass spectrometry-based serum metabolomics of kidney cancer | 2020 |
32 | A. Kołodziej; J. Nizioł; T. Ruman | Gold and silver nanoparticles-based laser desorption/ionization mass spectrometry method for detection and quantification of carboxylic acids | 2020 |
33 | B. Guratowska; J. Karbowska-Berent; T. Kozielec; T. Ruman; J. Szulc | Analyses of microorganisms and metabolites diversity on historic photographs using innovative methods | 2020 |
34 | B. Gutarowska; A. Jachowicz; S. Kowalska; W. Machnowski; T. Ruman; A. Steglinska; J. Szulc | Beeswax-Modified Textiles: Method of Preparation and Assessment of Antimicrobial Properties | 2020 |
35 | B. Gutarowska; I. Jablonskaja; E. Jabłońska; J. Karbowska-Berent; T. Ruman; J. Szulc | Metabolomics and metagenomics characteristic of historic beeswax seals | 2020 |
36 | I. Beech; J. Nizioł; A. Ossolińska; K. Ossoliński; T. Ossoliński; A. Płaza; T. Ruman; J. Sunner | Localization of Metabolites of Human Kidney Tissue with Infrared Laser-Based Selected Reaction Monitoring Mass Spectrometry Imaging and Silver-109 Nanoparticle-Based Surface Assisted Laser Desorption/Ionization Mass Spectrometry Imaging | 2020 |
37 | T. Ruman; J. Szulc | Laser Ablation Remote-Electrospray Ionisation Mass Spectrometry (LARESI MSI) Imaging—New Method for Detection and Spatial Localization of Metabolites and Mycotoxins Produced by Moulds | 2020 |
38 | A. Arendowski; J. Kucharz; J. Nizioł; A. Ossolińska; K. Ossoliński; T. Ossoliński; T. Ruman; P. Wiechno | Mass spectrometry-based metabolomic profiling of prostate cancer-a pilot study | 2019 |
39 | J. Cebulski; M. Kus-Liśkiewicz; T. Ruman; M. Stompor; D. Szmuc; K. Szmuc; Ł. Szyller; S. Wołowiec; I. Zawlik | Silver nanoparticles deposited on calcium hydrogenphosphate - silver phosphate matrix; biological activity of the composite | 2019 |
40 | M. Misiorek; J. Nizioł; T. Ruman | Mass spectrometry imaging of low molecular weight metabolites in strawberry fruit (Fragaria x ananassa Duch.) cv. Primoris with 109Ag nanoparticle enhanced target | 2019 |