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In vitro cultures


Some basic information about the module

Cycle of education:
2022/2023
The name of the faculty organization unit:
The faculty Chemistry
The name of the field of study:
Biotechnology
The area of study:
technical sciences
The profile of studing:
The level of study:
first degree study
Type of study:
past time
discipline specialities :
Applied biochemistry, Purification and analysis of biotechnological products
The degree after graduating from university:
Bachelor of Science (BSc)
The name of the module department :
Department of Biochemistry and Bioinformatics
The code of the module:
10940
The module status:
mandatory for teaching programme Applied biochemistry, Purification and analysis of biotechnological products
The position in the studies teaching programme:
sem: 4 / W9 L9 / 2 ECTS / Z
The language of the lecture:
Polish
The name of the coordinator:
Piotr Dziadczyk, PhD, Eng.

The aim of studying and bibliography

The main aim of study:

The general information about the module:

Bibliography required to complete the module
Bibliography used during lectures
1 Malepszy S. red Biotechnologia roślin PWN. 2014

Basic requirements in category knowledge/skills/social competences

Formal requirements:
non

Basic requirements in category knowledge:

Basic requirements in category skills:

Basic requirements in category social competences:

Module outcomes

MEK The student who completed the module Types of classes / teaching methods leading to achieving a given outcome of teaching Methods of verifying every mentioned outcome of teaching Relationships with KEK Relationships with PRK
MEK01 Can run besic types of in vitro cultures. Know the rools of sterile work lecture, laboratory Exam in wrighting, observation of performace, wrightrn raport, K-W14+++
K-U06+
K-U09++
K-U19+++
K-K03+++
 P6S-KR
P6S-UO
P6S-UU
P6S-UW
P6S-WG

The syllabus of the module

Sem. TK The content realized in MEK
4 TK01 Definition of plant in vitro culture. Application of plant in vitro culture W01 MEK01
4 TK02 Organisation of in vitro culture laboratory: equipment, rules of sterile work, W02 MEK01
4 TK03 Methods of terilization for glasware, media, tool. W03 MEK01
4 TK04 Media used in plant in vitro culture: types of media, ingredients (macro- microelemets, vitamins, plant hormones, aminoacids, sugars, geling agents). Composition and preparation of Murashige nad Skoog medium 1962. W04-W06 MEK01
4 TK05 Primary and secondary explants. Sources of primary explants. Methods of primary expalnts harvesting. W07 MEK01
4 TK06 Organogenesis in in vitro culture. Micropropagation as technological application of in vitro culture. W08-W09 MEK01
4 TK07 Kallus culture: induction, maintenance, application. W10 MEK01
4 TK08 Suspension culture: induction maintenance, application. W11 MEK01
4 TK09 Root culture. W12 MEK01
4 TK10 Application of in vitro culture in obtainig virus free plants. W13 MEK01
4 TK11 Anther culture. Microspore culture and production of dihaploids. W14 MEK01
4 TK12 Isolation, culture and fusion of plant protoplasts. W15 MEK01
4 TK13 Work safety L01
4 TK14 Rools of sterile work in plant in vitro culture laboratory. Operetion of equipment. L02 MEK01
4 TK15 Preparation of medium for carrot callus induction. L03-L05 MEK01
4 TK16 Induction of calli from primary explants of carrot L06-L07 MEK01
4 TK17 Preparation of medium for micropropafation of wild strawberry L08-L10 MEK01
4 TK18 Transpantation of wild strawberry microplants L11-L12 MEK01
4 TK19 Isolation of mature rye embryos. L13-L15 MEK01

The student's effort

The type of classes The work before classes The participation in classes The work after classes
Lecture (sem. 4) contact hours: 9.00 hours/sem.
 Studying the recommended bibliography: 5.00 hours/sem.
Laboratory (sem. 4) The preparation for a test: 10.00 hours/sem.
 contact hours: 9.00 hours/sem.
Advice (sem. 4) The participation in Advice: 2.00 hours/sem.
Credit (sem. 4) The preparation for a Credit: 15.00 hours/sem.
 The written credit: 2.00 hours/sem.

The way of giving the component module grades and the final grade

The type of classes The way of giving the final grade
Lecture
Laboratory
The final grade Marc from written exam.

Sample problems

Required during the exam/when receiving the credit
(-)

Realized during classes/laboratories/projects
(-)

Others
(-)

Can a student use any teaching aids during the exam/when receiving the credit : no

The contents of the module are associated with the research profile no