The aim of studying and bibliography

The main aim of study: Students are acquainting with techniques of purify biotechnological products.

The general information about the module: The student is getting the knowledge in the scope of mixtures separation and cleaning of a products with membrane, chromatography and extraction methods.

Bibliography required to complete the module

Bibliography used during lectures

1	R. Rautenbach	Procesy membranowe	WNT.	1996
2	W. Szewczyk	Technologia biochemiczna	Oficyna Wydawn. Pol. Warszawska.	2003
3	A. Selecki, R. Gawroński	Podstawy projektowania wybranych procesów rozdzielania mieszanin.	WNT.	1992
4	Antos Dorota, Kaczmarski Krzysztof, Piątkowski Wojciech	Chromatografia preparatywna jako proces rozdzielania mieszanin.	WNT.	2011

Bibliography used during classes/laboratories/others

1	praca zbiorowa pod red. R Petrusa	Inżynieria Chemiczna Laboratorium	Of. Wyd. PRz.	1998
2	W. Zapała, K. Kaczmarski, I. Poplewska, W. Piątkowski	Wybrane operacje jednostkowe w inżynierii procesowej	Of. Wyd. PRz.	2012

Basic requirements in category knowledge/skills/social competences

Formal requirements: registration for the given semester

Basic requirements in category knowledge: Student knows basic methods, techniques, tools and materials applied for solving basic problems connected with the biotechnology.

Basic requirements in category skills: Student has a skill for self-education.

Basic requirements in category social competences: NONE

Module outcomes

MEK	The student who completed the module	Types of classes / teaching methods leading to achieving a given outcome of teaching	Methods of verifying every mentioned outcome of teaching	Relationships with KEK	Relationships with PRK
01	Student is getting the knowledge about typpical unite operation proceses leading to isolation from the post-reaction mixture a required product. He knows physico-chemical principels of the separation and preparing the product with following methods: permeation, chromatography, extraction, drying and crystallization.	lecture	Colloquium	K_W10++	P6S_WG
02	He is able to separate required species with chromatography and membrane methods.	laboratory	pass all exercises	K_U17++	P6S_UO P6S_UW

Attention: Depending on the epidemic situation, verification of the achieved learning outcomes specified in the study program, in particular credits and examinations at the end of specific classes, can be implemented remotely (real-time meetings).

The syllabus of the module

Sem.	TK	The content	realized in	MEK
7	TK01	Strategies to recover and purify product. The permeate techniques of the mixtures separation: ultrafiltration, osmosis, reverse osmosis, microfiltration, dialysis, electrodialysis. Mathematical models of the processes. The examples of applications for species separation in biotechnology. Chromatographic and adsorptive technique of species separation. Thin layer chromatography, column periodical chromatography and continuous chromatography (SMB). Expanded bed adsorption chromatography. The normal and reversed phase chromatography. Ion exchange and gel chromatography. Theory of chromatographic separation: basic mathematical models of adsorption and mass transfer. The influence of process parameters: temperature, composition of mobile phase, solid phase, pH, ion strength of mobile phase on the mixtures separation. The optimization of periodical and continuous process. Principles of selections of chromatographic systems. Capillary electrophoresis and electrochromatography. Drying methods, crystallization methods.	A30 L15	MEK01 MEK02

The student's effort

The type of classes	The work before classes	The participation in classes	The work after classes
Lecture (sem. 7)	The preparation for a test: 2.00 hours/sem.	contact hours: 18.00 hours/sem.	complementing/reading through notes: 2.00 hours/sem.
Laboratory (sem. 7)	The preparation for a Laboratory: 2.00 hours/sem. The preparation for a test: 2.00 hours/sem.	contact hours: 9.00 hours/sem.	Finishing/Making the report: 2.00 hours/sem.
Advice (sem. 7)
Credit (sem. 7)	The preparation for a Credit: 15.00 hours/sem.	The written credit: 2.00 hours/sem.

The way of giving the component module grades and the final grade

The type of classes	The way of giving the final grade
Lecture	test
Laboratory	Arithmetic mean from grades of exercises
The final grade	FE = 0.6L+0.4Lab. w - coefficient taking into account the term when positive course credit was obtained, w = 1,0 first term, w = 0,9 second term, w = 0,8 third term.

Sample problems

Required during the exam/when receiving the credit
(-)

Realized during classes/laboratories/projects
(-)

Others
(-)

Can a student use any teaching aids during the exam/when receiving the credit : no

The contents of the module are associated with the research profile: yes

1	D. Antos; W. Piątkowski	Equilibria and kinetics of ion-exchange	2024
2	D. Antos; M. Balawejder; J. Gumieniak; P. Mruc; M. Olbrycht; W. Piątkowski	Separation of non-racemic mixtures of enantiomers by achiral chromatography	2023
3	D. Antos; M. Kołodziej; W. Piątkowski; T. Rumanek	Preferential precipitation of acidic variants from monoclonal antibody pools	2023
4	D. Antos; M. Kołodziej; W. Piątkowski; T. Rumanek; P. Zimoch	Coupling of chromatography and precipitation for adjusting acidic variant content in a monoclonal antibody pool	2023
5	D. Antos; W. Piątkowski	Kinetic and Thermodynamic Aspects of Hydrophobic Interaction Chromatography	2023
6	D. Antos; A. Bajek-Bil; M. Balawejder; M. Olbrycht; W. Piątkowski	Sposób otrzymywania stereoizomeru szczawianu nafronylu o konfiguracji absolutnej (2S, 2\'R)	2021
7	D. Antos; A. Bajek-Bil; M. Balawejder; M. Olbrycht; W. Piątkowski; I. Poplewska	Development of a Route to the Most Active Nafronyl Stereoisomer by Coupling Asymmetric Synthesis and Chiral Chromatography Separation	2021
8	D. Antos; K. Baran; W. Piątkowski; A. Stańczak; P. Zimoch	Separation of charge variants of a monoclonal antibody by overloaded ion exchange chromatography	2021
9	D. Antos; P. Antos; M. Balawejder; R. Bochenek; J. Gorzelany; K. Kania; M. Kołodziej; N. Matłok; M. Olbrycht; W. Piątkowski; M. Przywara; G. Witek	Sposób wytwarzania nawozu wieloskładnikowego o kontrolowanym uwalnianiu składników	2021
10	D. Antos; W. Piątkowski; I. Poplewska	A case study of the mechanism of unfolding and aggregation of a monoclonal antibody in ion exchange chromatography	2021
11	D. Antos; G. Carta; M. Kołodziej; R. Muca; W. Piątkowski	Effects of negative and positive cooperative adsorption of proteins on hydrophobic interaction chromatography media	2020
12	D. Antos; J. Beck; A. Durauer; R. Hahn; A. Jungbauer; M. Kołodziej; W. Marek; W. Piątkowski; D. Sauer	Scale up of a chromatographic capture step for a clarified bacterial homogenate - Influence of mass transport limitation and competitive adsorption of impurities	2020
13	D. Antos; P. Antos; M. Balawejder; R. Bochenek; M. Kołodziej; N. Matłok; M. Olbrycht; W. Piątkowski; M. Przywara	Mechanism of nutrition activity of a microgranule fertilizer fortified with proteins	2020
14	D. Antos; K. Baran; W. Marek; W. Piątkowski	Effect of flow behavior in extra-column volumes on the retention pattern of proteins in a small column	2019
15	D. Antos; M. Balawejder; H. Lorenz; M. Olbrycht; W. Piątkowski; I. Poplewska; A. Seidel-Morgenstern	Cooperative Kinetic Model to Describe Crystallization in Solid Solution Forming Systems	2019
16	D. Antos; M. Kołodziej; A. Łyskowski; W. Piątkowski; I. Poplewska; P. Szałański	Determination of protein crystallization kinetics by a through-flow small-angle X-ray scattering method	2019
17	D. Antos; P. Antos; M. Balawejder; R. Bochenek; J. Gorzelany; K. Kania; M. Kołodziej; N. Matłok; M. Olbrycht; W. Piątkowski; M. Przywara; G. Witek	Sposób wytwarzania nawozu wieloskładnikowego o kontrolowanym uwalnianiu składników	2019