The aim of studying and bibliography

The main aim of study: Acquaint students with: a) the basics of genetics, breeding and physiology of plants including flowering biology, b) methods for generating transgenic plants and the main trends and prospects of their use, c) practical applications of in vitro cultures in plant production, the biosynthesis of secondary metabolites and the production of research materials d) methods of modern molecular diagnostics in the protection and plant breeding, and in basic genetic research, e) new research tools in understanding the plant genome. Familiarization with selected biochemical processes specific to the plant world. Understanding the importance and preservation of genetic biodiversity. Knowledge of basic methods of PCR analyzes of plants Practical skills of working with plant material: the isolation of DNA, documentation of test results, setting PCR (RAPD, ISSR, SSR, CAPS) and the detection of point mutations. Separation on agarose gel and polyacrylamide. Elements of planning research and interpretation of the results.

The general information about the module: The module is implemented in the sixth semester includes 30 hours of lectures and 15 hours laboratory. The module ends with an exam.

Teaching materials: Instrukcje laboratoryjne na stronach domowych pracowników prowadzących

Bibliography required to complete the module

Bibliography used during lectures

1	Malepszy S.	Biotechnologia roślin	PWN Warszawa.	2004
2	Malepszy S.	Biotechnologia roślin	PWN Warszawa.	2009
3	Buchowicz J	Biotechnologia molekularna: modyfikacje genetyczne, postępy, problemy	Wydaw.Nauk.PWN.	2009
4	Glick, Bernard R..	Molecular biotechnology : principles and applications of recombinant DNA.	Washington : ASM Press.	2003

Basic requirements in category knowledge/skills/social competences

Formal requirements: registration for the semester

Basic requirements in category knowledge: Practical skills acquired previously during laboratory classes in the use of automatic pipettes and knowledge of the rules of behavior in the laboratory.

Basic requirements in category skills: Practical skills acquired previously during laboratory classes in the use of automatic pipettes and knowledge of the rules of behavior in the laboratory.

Basic requirements in category social competences: Competence for the safe use of equipment, research laboratories.

Module outcomes

MEK	The student who completed the module	Types of classes / teaching methods leading to achieving a given outcome of teaching	Methods of verifying every mentioned outcome of teaching	Relationships with KEK	Relationships with PRK
01	Knows the rules of expression and inheritance of genetic information and molecular techniques used to study the genetic material of plants.	lecture	written exam	K_W06+++ K_W09+++ K_W14++ K_K02++ K_K07+	P6S_KO P6S_KR P6S_WG
02	Has a general understanding of the current directions of development of plant biotechnology and modern research methods.	lecture	written exam	K_W12++ K_U03+++	P6S_UK P6S_WG
03	Able to plan an experiment in genetic engineering and biotechnology, properly execute it, interpret the results and draw conclusions	laboratory	pass of the practical part	K_U09++ K_U18+ K_U19++	P6S_UO P6S_UW

Attention: Depending on the epidemic situation, verification of the achieved learning outcomes specified in the study program, in particular credits and examinations at the end of specific classes, can be implemented remotely (real-time meetings).

The syllabus of the module

Sem.	TK	The content	realized in	MEK
5	TK01	Genetics and Biotechnology. Elements of population genetics, genetics and plant breeding. Cytogenetics in plant biotechnology. Molecular diagnosis of plant and pathogen. Genomics research plants. Feedback and gene mapping. Isolation and characterization of genes. Transgenic plants - methods of transformation, identification and breeding. The cell wall - structure and improve biotechnology.	W01-W10	MEK01
5	TK02	The concept of biotechnology. Biomass feedstock biotechnology. Biotransformation of selected chemicals. Plant biotechnology to improve the quality of food, modified starch and other carbohydrates. The transgenic plants as a source of modified oils of storage proteins with improved functional properties. Use of bioreactor cultures of plant cells and tissue. Production of immunotherapeutic agents and biopharmaceuticals in plants. Production of bio fuel.	W11-W20	MEK02
5	TK03	Regulation of physiological processes, plant growth and development by endogenous and exogenous factors. Creating a structure gene in plant transformation. Industrial strategies for detection of bioactive compounds in plants. Transgenic plants in improving resistance to biotic, abiotic and herbicides. Transformations and functions of lipids (waxes, cutin and suberin).	W21-W30	MEK03

The student's effort

The type of classes	The work before classes	The participation in classes	The work after classes
Lecture (sem. 5)		contact hours: 18.00 hours/sem.	complementing/reading through notes: 10.00 hours/sem. Studying the recommended bibliography: 10.00 hours/sem.
Laboratory (sem. 5)	The preparation for a Laboratory: 10.00 hours/sem. The preparation for a test: 10.00 hours/sem.	contact hours: 9.00 hours/sem.	Finishing/Making the report: 10.00 hours/sem.
Advice (sem. 5)		The participation in Advice: 2.00 hours/sem.
Exam (sem. 5)	The preparation for an Exam: 20.00 hours/sem.	The written exam: 2.00 hours/sem.

The way of giving the component module grades and the final grade

The type of classes	The way of giving the final grade
Lecture
Laboratory
The final grade

Sample problems

Required during the exam/when receiving the credit
(-)

Realized during classes/laboratories/projects
(-)

Others
(-)

Can a student use any teaching aids during the exam/when receiving the credit : no

The contents of the module are associated with the research profile: yes

1	B. Bakera; M. Rakoczy-Trojanowska; M. Szeliga; M. Święcicka; M. Tyrka	Identification of candidate genes responsible for chasmogamy in wheat	2023
2	P. Bednarek; A. Dorczyk; T. Drzazga; D. Jasińska; P. Krajewski; B. Ługowska; R. Martofel; P. Matysik; M. Niewińska; D. Ratajczak; K. Rączka; T. Sikora; D. Tyrka; M. Tyrka; E. Witkowski; U. Woźna-Pawlak	Genome-wide association mapping in elite winter wheat breeding for yield improvement	2023
3	M. Dyda; G. Gołębiowska; M. Rapacz; M. Szechyńska-Hebda; M. Tyrka; I. Wąsek; M. Wędzony	Quantitative trait loci and candidate genes associated with freezing tolerance of winter triticale (× Triticosecale Wittmack)	2022
4	M. Dyda; G. Gołębiowska; M. Rapacz; M. Tyrka; M. Wędzony	Genetic mapping of adult-plant resistance genes to powdery mildew in triticale	2022
5	M. Dyda; G. Gołębiowska; M. Rapacz; M. Tyrka; M. Wędzony	Mapping of QTL and candidate genes associated with powdery mildew resistance in triticale (× Triticosecale Wittm.)	2022
6	P. Krajewski; R. Marcinkowski; R. Martofel; P. Matysik; M. Mokrzycka; M. Rakoczy-Trojanowska; M. Rokicki; S. Stojałowski; M. Tyrka; U. Woźna-Pawlak; B. Żmijewska	Genome-Wide Association Analysis for Hybrid Breeding in Wheat	2022
7	A. Pietrusińska; M. Tyrka	Linkage of Lr55 wheat leaf rust resistance gene with microsatellite and DArT-based markers	2021
8	B. Bakera; P. Krajewski; M. Mokrzycka; M. Rakoczy-Trojanowska; M. Szeliga; M. Święcicka; M. Tyrka	Identification of Rf Genes in Hexaploid Wheat (Triticumaestivum L.) by RNA-Seq and Paralog Analyses	2021
9	B. Bakera; P. Krajewski; P. Matysik; M. Mokrzycka; M. Rakoczy-Trojanowska; M. Rokicki; S. Stojałowski; M. Szeliga; D. Tyrka; M. Tyrka	Evaluation of genetic structure in European wheat cultivars and advanced breeding lines using high-density genotyping-by-sequencing approach	2021
10	J. Buczkowicz; T. Drzazga; B. Ługowska; P. Matysik; K. Rubrycki; M. Semik; D. Tyrka; M. Tyrka; E. Witkowski	Identyfikacja efektywnych genów odporności na wybrane choroby wirusowe i grzybowe pszenicy zwyczajnej	2021
11	J. Buczkowicz; T. Drzazga; G. Fic; M. Jaromin; P. Krajewski; P. Matysik; R. Mazur; P. Milczarski; T. Sikora; M. Szeliga; D. Tyrka; M. Tyrka; E. Witkowski	Selekcja genomowa pszenicy ozimej	2021
12	E. Ciszkowicz; E. Kaznowska; P. Porzycki; M. Semik; M. Tyrka	MiR-93/miR-375: Diagnostic Potential, Aggressiveness Correlation and Common Target Genes in Prostate Cancer	2020
13	G. Czajowski; M. Karbarz; M. Pojmaj; A. Strzembicka; D. Tyrka; M. Tyrka; A. Wardyńska; M. Wędzony	Quantitative trait loci mapping of adult-plant resistance to powdery mildew in triticale	2020
14	J. Ciura; M. Szeliga; M. Tyrka	Representational Difference Analysis of Transcripts Involved in Jervine Biosynthesis	2020
15	J. Ciura; M. Grzesik; M. Szeliga; M. Tyrka	Identification of candidate genes involved in steroidal alkaloids biosynthesis in organ-specific transcriptomes of Veratrum nigrum L.	2019
16	M. Dyda; M. Szechyńska-Hebda; M. Tyrka; I. Wąsek; M. Wędzony	Local and systemic regulation of PSII efficiency in triticale infected by the hemibiotrophic pathogen Microdochium nivale	2019
17	M. Dziurka; K. Hura; T. Hura; A. Ostrowska; M. Tyrka	Participation of Wheat and Rye Genome in Drought Induced Senescence in Winter Triticale (X Triticosecale Wittm.)	2019
18	Z. Banaszak; A. Fiust; Z. Nita; W. Orłowska-Job; M. Pojmaj; M. Rapacz; M. Tyrka; M. Wójcik-Jagła	Sposób selekcji mrozoodpornych genotypów jęczmienia ozimego	2019