The aim of studying and bibliography

The main aim of study: Understanding of roles governing the system of genetic information transfer fro generation to generation, understanding ways in which genes gover biochemical i fiziological proceses in cells. Learnic besic methods applied in molecular biology laboratories.

The general information about the module: 45h of lectures 45h of laboratories Divided in the following way: 30h of lecures and 30h of laboratories in the winter semester and 15h of lectures and 15h of laboratories in summer semester.

Bibliography required to complete the module

Bibliography used during lectures

1	James D. Watson, Tania A. Baker, Stephen P. Bell	Molecular Biology of the Gene	Pearson.	2013
2	Marcelo L. Larramendy and Sonia Soloneski	Nucleic Acids: From Basic Aspects to Laboratory Tools	ITexli.	2016

Bibliography used during classes/laboratories/others

1	Muller	Nucleic Acids from A to Z	2008.	Wiley
2	Westermeier	Electrophoresis in Practice: A Guide to Methods and Applications of DNA and Protein Separations	Viley.	2016

Bibliography to self-study

1

Alexander McLenann, Andy Bates, Mike White, Phil Turner

Krótkie wykłady Biologia molekularna

PWN.

2011

Basic requirements in category knowledge/skills/social competences

Formal requirements: none

Basic requirements in category knowledge: Knowlege of cell biology, genetics and biochemistry

Basic requirements in category skills: basic laboratory skills

Basic requirements in category social competences: non

Module outcomes

MEK	The student who completed the module	Types of classes / teaching methods leading to achieving a given outcome of teaching	Methods of verifying every mentioned outcome of teaching	Relationships with KEK	Relationships with PRK
01	Knows molecular mechanisms kontrolinng functions of the cells: replication, transcription , translation, DNA repare	lecture, laboratory	exam in wrighting, observation of performance., written report,	K_W05+++ K_W06+++ K_W14+++ K_U06+++ K_U09+ K_K01+++ K_K03+++	P6S_KK P6S_KR P6S_UO P6S_UU P6S_UW P6S_WG
02	Knows basic methods of nucleic acids analisys : isolation, electrophoresis, restriction, ligation, hybridyzation RCR.	lecture , laboratory	exam, observation of performace, written report	K_W05+ K_W14+ K_U09+ K_K01+ K_K03+	P6S_KK P6S_KR P6S_UO P6S_UW P6S_WG
03	Knows practical possibilities of using knowlage from the field of molecular biology in biotechnology, in production of medicines, cosmetics, foods and medical analitics.	lecture laboratories	Exame	K_W05+ K_W14+ K_K01+ K_K03+	P6S_KK P6S_KR P6S_WG

Attention: Depending on the epidemic situation, verification of the achieved learning outcomes specified in the study program, in particular credits and examinations at the end of specific classes, can be implemented remotely (real-time meetings).

The syllabus of the module

Sem.	TK	The content	realized in	MEK
5	TK01	Basic terminology in the field of molecular biology. Differences in the structure of genetic information between pro and eucariots. Introduction to labolatory procedures - isolation of nucleic acids.	W01-W04	MEK01 MEK02 MEK03
5	TK02	Plasmids: structure, replication, biological function, transfer of information between cells, resiatance to unfavorable environmental conditions like antibitics, heave metal ions, sulfonamids, phenol and its derivatives. Virulence towards host, elimination of competitors from environment. Systematcs of pasmids. Application of plasmids in genetic ingeniering; Ti, Ri, E. coli plasmids.Introduction to laboratorie; restriction enzymes, restriction mappiong	W05-W12	MEK01 MEK02 MEK03
5	TK03	Structure of the bacterial chromosome. Recplication of the bacterial chromosome. Hetthylation of bacteroal chromosome. RCR.	W13-W17	MEK01 MEK02 MEK03
5	TK04	Transcription in procariots	W18-W20	MEK01 MEK02 MEK03
5	TK05	Structure and function of bacterial ribosoms. Translation in procariotic cells. Posttranslational modification of proteins.	W21-W23	MEK01 MEK02 MEK03
5	TK06	Sources of diversity in microorganisms.	W24-W25	MEK01 MEK02 MEK03
5	TK07	Compartmentalization of eucariotic cells and its influence on structure of eucariotic genomes.	W26	MEK01 MEK02 MEK03
5	TK08	Structure of eucariotic chromosom: centromer, telomers, eucgromatin, heterochromatin, nucleosom, histones. Replication of eucariotic chromosom.	W27-W30	MEK01
5	TK09	E. coli plasmids isolation.	L01-L05	MEK01 MEK02 MEK03
5	TK10	DNA electroforeis in agarose gel.	L06-L10	MEK01 MEK02 MEK03
5	TK11	Digestion of DNA with restriction enzymes.	L11-L15	MEK01 MEK02 MEK03
5	TK12	PCR	L16-L20	MEK01 MEK02 MEK03
5	TK13	Restriction mapping , aanalizys of PCR products.	L21-L25	MEK01 MEK02 MEK03
5	TK14	DNA ligation	L26-L30	MEK01 MEK02 MEK03
6	TK01	-	W01	MEK01 MEK02 MEK03
6	TK02	-	W02	MEK01 MEK02 MEK03
6	TK03	-	W03-W05	MEK01 MEK02 MEK03
6	TK04	-	W06-Wo7	MEK01 MEK02 MEK03
6	TK05	-	W08	MEK01 MEK02 MEK03
6	TK06	-	W09-W10	MEK01 MEK02 MEK03
6	TK07	-	W11-W12	MEK01 MEK02 MEK03
6	TK08	-	W13-W14	MEK01 MEK02 MEK03
6	TK09	-	W15	MEK01 MEK02 MEK03
6	TK10	-	L01-L05	MEK01 MEK02 MEK03
6	TK11	-	L06-L15	MEK01 MEK02 MEK03

The student's effort

The type of classes	The work before classes	The participation in classes	The work after classes
Lecture (sem. 5)		contact hours: 18.00 hours/sem.	complementing/reading through notes: 10.00 hours/sem. Studying the recommended bibliography: 15.00 hours/sem.
Laboratory (sem. 5)	The preparation for a Laboratory: 15.00 hours/sem. The preparation for a test: 15.00 hours/sem.	contact hours: 18.00 hours/sem.	Finishing/Making the report: 10.00 hours/sem.
Advice (sem. 5)		The participation in Advice: 2.00 hours/sem.
Exam (sem. 5)	The preparation for an Exam: 25.00 hours/sem.	The written exam: 1.00 hours/sem.
Lecture (sem. 6)		contact hours: 9.00 hours/sem.	complementing/reading through notes: 2.00 hours/sem. Studying the recommended bibliography: 10.00 hours/sem.
Laboratory (sem. 6)		contact hours: 9.00 hours/sem.
Advice (sem. 6)
Credit (sem. 6)	The preparation for a Credit: 20.00 hours/sem.	The written credit: 1.00 hours/sem.

The way of giving the component module grades and the final grade

The type of classes	The way of giving the final grade
Lecture	marc from exam
Laboratory	score
The final grade
Lecture	marc from exam
Laboratory	score
The final grade	marc from exam

Sample problems

Required during the exam/when receiving the credit
(-)

Realized during classes/laboratories/projects
Pytania biologia molekularna.pdf

Others
(-)

Can a student use any teaching aids during the exam/when receiving the credit : no

The contents of the module are associated with the research profile: no