The aim of studying and bibliography

The main aim of study: To provide students with the basic methods of instrumental analysis, construction and operation of the equipment and its use for the determination of the composition, purity of biotechnology products and materials.

The general information about the module:

others: Instrukcje do ćwiczeń laboratoryjnych

Bibliography required to complete the module

Bibliography used during lectures

1	K. Danzer i inn.	Analityka – przegląd systematyczny	WNT, Warszawa .	1993
2	A. Cygański	Metody spektroskopowe w chemii analitycznej	WNT, Warszawa.	1997
3	E. Hoffmann, J. Charette, V. Stroobant	Spektrometria mas	WNT, Warszawa.	1998
4	A. Cygański	Metody elektroanalityczne	WNT, Warszawa.	1995
5	Z. Witkiewicz	Podstawy chromatografii	WNT, Warszawa.	2005

Bibliography used during classes/laboratories/others

1	W. Szczepaniak	Metody instrumentalne w analizie chemicznej	PWN, Warszawa.	2004
2	R.M. Silverstein i inn.	Spektroskopowe metody identyfikacji związków organicznych	PWN, Warszawa.	2007

Bibliography to self-study

1

W. Szczepaniak

Metody instrumentalne w analizie chemicznej

PWN, Warszawa.

2004

Basic requirements in category knowledge/skills/social competences

Formal requirements: completed course of general chemistry, analytical chemistry,and physical chemistry

Basic requirements in category knowledge: Knowledge of physics, analytical, organic and physical chemistry.

Basic requirements in category skills: Basic skills relating to the calculation of analyte concentration, preparing solutions and the use of the chemical laboratory equipment.

Basic requirements in category social competences: Knowledge of safety rules in the chemical laboratory, the responsibility required during chemical experiments performed individually and in a group.

Module outcomes

MEK	The student who completed the module	Types of classes / teaching methods leading to achieving a given outcome of teaching	Methods of verifying every mentioned outcome of teaching	Relationships with KEK	Relationships with PRK
01	Has knowledge about theoretical foundations of the common intrumental methods of analysis, principles of operation and construction of modern analytical instrumentation.	lecture, laboratory	written test and report for every laboratory exercise, observation of performance, pass a final written test	K_W04++	P6S_WG
02	Acquired knowlewdge on the application of the various instrumental techniques to the solution of analytical problems in experimental research, biotechnology and chemical production.	lecture	pass a final written test	K_W04++ K_W10+	P6S_WG
03	Able to select and justify the use of instrumental methods of analysis in terms of analytical and economic requirements.	lecture, laboratory	written test and report for every laboratory exercise, observation of performance, pass a final written test	K_U17+	P6S_UO P6S_UW
04	Mastered the practical usage of the instrumental methods to perform chemical analyses	laboratory	written report for every laboratory exercise, observation of performance	K_U16++	P6S_UO P6S_UW
05	Knows and can practically use the qualitative and quantitative methods of analysis, and calibration techniques.	lecture, laboratory	written test and report for every laboratory exercise, observation of performance, pass a final written test	K_U16+	P6S_UO P6S_UW
06	Knows how to prepare a report on laboratory exercises, is able to evaluate the results and draw valid conclusions.	laboratory	laboratory report
07	Has ability to work independently and to interact in a team.	laboratory	observation of performance
08	Knows the principles of good laboratory practice, including the principles of safety, waste collection.
09	Understands the need for continuous complement of knowledge about the new solutions and methods of instrumental analysis.	lecture, laboratory		K_K01++	P6S_KK P6S_KR

Attention: Depending on the epidemic situation, verification of the achieved learning outcomes specified in the study program, in particular credits and examinations at the end of specific classes, can be implemented remotely (real-time meetings).

The syllabus of the module

Sem.	TK	The content	realized in	MEK
5	TK01	Analytical process, its elements and statistical evaluation of each step. Analysis of elements and compounds by spectroscopic methods. Atomic Emission Spectroscopy - basis of the method, methods of sample atomization and excitation, applications. Atomic absorption spectroscopy. Molecular spectroscopy in the ultraviolet and visible light. Infrared spectroscopy. Spectra recording techniques, methods of quantitative and qualitative analysis. Fundamentals of nuclear magnetic resonance spectroscopy. The quantitative and structural analysis based on the NMR spectra. Fundamentals of mass spectrometry. Interpretation and application of analytical mass spectra for organic compounds. Chromatographic methods for separation of mixtures. Basic principles and classification. Theoreticasl basis of separation process. Retention mechanisms and parameters. Separation efficiency. Definition and determination of resolution index, theoretical plate number, selectivity factor. Separation techniques in liquid chromatography - adsorption chromatography, partition - normal/reverse chromatography, ion-exchange chromatography , gel filtration chromatography. Selection of the chromatographic conditions - rules for the selection of the stationary and mobile phases. High Performance Liquid Chromatography HPLC and thin-layer HPTLC. Isocratic and gradient techniques, instrumentation. Gas chromatography. The rate theory of chromatography - band broadening, column efficiency. Optimization of column performance. Chromatographic methods of qualitative and quantitative analysis. Potentiometric methods. Design, operation and application of the selected ion-selective electrodes. Conductometry and its analytical application. Voltammetric methods - linear-sweep LSV, cyclic CV, and stripplng CSV, ASV techniques. Quantitative and qualitative analysis. Selected applications in analytical laboratory and industrial applications, criteria for the method selection.	W30	MEK01 MEK02 MEK03 MEK05 MEK09
5	TK02	Identification of components in the mixture of hydrocarbons and their determination by gas chromatography GC. Determination of hydrocarbons and their derivatives using HPLC. Analysis of the composition of mixtures of organic compounds using a GC-MS. Identification and a quantitative analysis by IR spectroscopy. Determination of the concentration of substances bythe UV-VIS spectroscopy. Structural analysis on the base of 1H-NMR spectra. Determination of the element content in the solutions by atomic absorption spectroscopy (AAS). Polarimetric determination of sucrose in aqueous solution. Quantitative determination of elements by cyclic voltammetry CV. Determination of iodide and chloride by potentiometric precipitation titration. Determination of the concentration of the phenol by conductrometric titration method.	L45	MEK01 MEK03 MEK04 MEK05 MEK06 MEK07 MEK08 MEK09

The student's effort

The type of classes	The work before classes	The participation in classes	The work after classes
Lecture (sem. 5)		contact hours: 18.00 hours/sem.	complementing/reading through notes: 5.00 hours/sem. Studying the recommended bibliography: 15.00 hours/sem.
Laboratory (sem. 5)	The preparation for a Laboratory: 5.00 hours/sem. Others: 15.00 hours/sem.	contact hours: 27.00 hours/sem.	Finishing/Making the report: 20.00 hours/sem.
Advice (sem. 5)	The preparation for Advice: 2.00 hours/sem.	The participation in Advice: 2.00 hours/sem.
Credit (sem. 5)	The preparation for a Credit: 15.00 hours/sem.	The written credit: 2.00 hours/sem.

The way of giving the component module grades and the final grade

The type of classes	The way of giving the final grade
Lecture
Laboratory
The final grade

Sample problems

Required during the exam/when receiving the credit
(-)

Realized during classes/laboratories/projects
(-)

Others
(-)

Can a student use any teaching aids during the exam/when receiving the credit : no

The contents of the module are associated with the research profile: yes

1	D. Naróg; A. Sobkowiak	Electrochemistry of Flavonoids	2023
2	P. Chmielarz; T. Pacześniak; K. Rydel-Ciszek; A. Sobkowiak	Bio-Inspired Iron Pentadentate Complexes as Dioxygen Activators in the Oxidation of Cyclohexene and Limonene	2023
3	Ł. Florczak; B. Kościelniak; A. Kramek; A. Sobkowiak	The Influence of Potassium Hexafluorophosphate on the Morphology and Anticorrosive Properties of Conversion Coatings Formed on the AM50 Magnesium Alloy by Plasma Electrolytic Oxidation	2023
4	D. Naróg; A. Sobkowiak	Electrochemical Investigation of some Flavonoids in Aprotic Media	2022
5	K. Darowicki; Ł. Florczak; G. Nawrat; K. Raga; J. Ryl; J. Sieniawski; A. Sobkowiak; M. Wierzbińska	The Effect of Sodium Tetrafluoroborate on the Properties of Conversion Coatings Formed on the AZ91D Magnesium Alloy by Plasma Electrolytic Oxidation	2022
6	A. Baran; M. Drajewicz; A. Dryzner; M. Dubiel; Ł. Florczak; M. Kocój-Toporowska; A. Krząkała; K. Kwolek; P. Kwolek; G. Lach; G. Nawrat; Ł. Nieużyła; K. Raga; J. Sieniawski; A. Sobkowiak; T. Wieczorek	Method of Forming Corrosion Resistant Coating and Related Apparatus	2021
7	P. Chmielarz; A. Miłaczewska; T. Pacześniak; K. Rydel-Ciszek; A. Sobkowiak	‘Oxygen-Consuming Complexes’–Catalytic Effects of Iron–Salen Complexes with Dioxygen	2021
8	D. Naróg	Electrochemical study of quercetin in the presence of galactopyranose: Potential application to the electrosynthesis of glycoconjugates of quinone/quinone methide of quercetin	2020
9	P. Błoniarz; P. Chmielarz; T. Pacześniak; K. Rydel-Ciszek; A. Sobkowiak; K. Surmacz; I. Zaborniak	Iron-Based Catalytically Active Complexes in Preparation of Functional Materials	2020
10	P. Chmielarz; A. Gennaro; G. Grześ; A. Isse; A. Sobkowiak; K. Wolski; I. Zaborniak; S. Zapotoczny	Tannic acid-inspired star-like macromolecules via temporally-controlled multi-step potential electrolysis	2019