The aim of studying and bibliography

The main aim of study: Teaching studets about methods of protein purification

The general information about the module: The course consist of lecture and laboratory. It concerns the most typical methods isolating and purification of proteins in industrial applications.

Bibliography required to complete the module

Bibliography used during lectures

1	Giorgio Carta, Alois Jungbauer	Protein Chromatography: Process Development and Scale-Up	Wiley.	2010
2	D. Antos, K. Kaczmarski, W. Piątkowski	Chromatografia preparatywna jako proces rozdzielania mieszanin	WNT.	2011

Basic requirements in category knowledge/skills/social competences

Formal requirements: registration for 2 semester

Basic requirements in category knowledge: Knowledge about basic unit operations in chemical and process engineering

Basic requirements in category skills:

Basic requirements in category social competences: student is able to work in a team

Module outcomes

MEK	The student who completed the module	Types of classes / teaching methods leading to achieving a given outcome of teaching	Methods of verifying every mentioned outcome of teaching	Relationships with KEK	Relationships with PRK
01	Students gain knowledge about the most important unit operation used for isolation and purification of biologically active compounds such as chromatography, precipitation and crystallization	lecture, laboratory	written test	K_W02+ K_W07+ K_U09+	P7S_UO P7S_UW P7S_WG

Attention: Depending on the epidemic situation, verification of the achieved learning outcomes specified in the study program, in particular credits and examinations at the end of specific classes, can be implemented remotely (real-time meetings).

The syllabus of the module

Sem.	TK	The content	realized in	MEK
2	TK01	Specific aspects concerning unit operations used in purification of bioactive compounds. Isolation of proteins by use of chromatographic techniques: hydrophobic interaction chromatography, ion-exchange, size exclusion chromatography, affinity chromatography. Specific and non-specific precipitation,. Separation of pharmaceutically by crystallization.	-	MEK01

The student's effort

The type of classes	The work before classes	The participation in classes	The work after classes
Lecture (sem. 2)	The preparation for a test: 10.00 hours/sem.	contact hours: 9.00 hours/sem.	complementing/reading through notes: 5.00 hours/sem. Studying the recommended bibliography: 6.00 hours/sem.
Laboratory (sem. 2)	The preparation for a Laboratory: 1.00 hours/sem. The preparation for a test: 7.00 hours/sem.	contact hours: 9.00 hours/sem.	Finishing/Making the report: 2.00 hours/sem.
Advice (sem. 2)		The participation in Advice: 1.00 hours/sem.
Credit (sem. 2)		The written credit: 1.00 hours/sem.

The way of giving the component module grades and the final grade

The type of classes	The way of giving the final grade
Lecture	On the basis of the mark obtained in colloquium
Laboratory	On the basis of the mark obtained in colloquium
The final grade	The final mark is calulated as follows: OK= 65% OW + 35% OL, OW - mark for lecture, OL - mark for laboratory

Sample problems

Required during the exam/when receiving the credit
(-)

Realized during classes/laboratories/projects
(-)

Others
(-)

Can a student use any teaching aids during the exam/when receiving the credit : no

The contents of the module are associated with the research profile: yes

1	D. Antos; M. Korbetskyy; P. Mruc; M. Olbrycht	Altering the mobile phase composition to enhance self-disproportionation of enantiomers in achiral chromatography	2024
2	D. Antos; W. Piątkowski	Equilibria and kinetics of ion-exchange	2024
3	D. Antos; M. Balawejder; J. Gumieniak; P. Mruc; M. Olbrycht; W. Piątkowski	Separation of non-racemic mixtures of enantiomers by achiral chromatography	2023
4	D. Antos; M. Kołodziej; W. Piątkowski; T. Rumanek	Preferential precipitation of acidic variants from monoclonal antibody pools	2023
5	D. Antos; M. Kołodziej; W. Piątkowski; T. Rumanek; P. Zimoch	Coupling of chromatography and precipitation for adjusting acidic variant content in a monoclonal antibody pool	2023
6	D. Antos; R. Bochenek; B. Filip; W. Marek	Flow behavior of protein solutions in a lab-scale chromatographic system	2023
7	D. Antos; R. Dürr; A. Kienle; E. Otto; M. Przywara	Modeling of particle formation in pan granulators with sieve-mill recycle	2023
8	D. Antos; R. Muca	Protein association on multimodal chromatography media	2023
9	D. Antos; W. Piątkowski	Kinetic and Thermodynamic Aspects of Hydrophobic Interaction Chromatography	2023
10	D. Antos; I. Poplewska; P. Zimoch	Dissociation events during processing of monoclonal antibodies on strong cation exchange resins	2022
11	D. Antos; A. Bajek-Bil; M. Balawejder; M. Olbrycht; W. Piątkowski	Sposób otrzymywania stereoizomeru szczawianu nafronylu o konfiguracji absolutnej (2S, 2\'R)	2021
12	D. Antos; A. Bajek-Bil; M. Balawejder; M. Olbrycht; W. Piątkowski; I. Poplewska	Development of a Route to the Most Active Nafronyl Stereoisomer by Coupling Asymmetric Synthesis and Chiral Chromatography Separation	2021
13	D. Antos; K. Baran; R. Bochenek; B. Filip; D. Strzałka	Influence of the geometry of extra column volumes on band broadening in a chromatographic system. Predictions by computational fluid dynamics	2021
14	D. Antos; K. Baran; W. Piątkowski; A. Stańczak; P. Zimoch	Separation of charge variants of a monoclonal antibody by overloaded ion exchange chromatography	2021
15	D. Antos; P. Antos; M. Balawejder; R. Bochenek; J. Gorzelany; K. Kania; M. Kołodziej; N. Matłok; M. Olbrycht; W. Piątkowski; M. Przywara; G. Witek	Sposób wytwarzania nawozu wieloskładnikowego o kontrolowanym uwalnianiu składników	2021
16	D. Antos; R. Dürr; A. Kienle; E. Otto; M. Przywara	Population Balance Modelling of Pan Granulation Processes	2021
17	D. Antos; R. Dürr; A. Kienle; E. Otto; M. Przywara	Process Behavior and Product Quality in Fertilizer Manufacturing Using Continuous Hopper Transfer Pan Granulation—Experimental Investigations	2021
18	D. Antos; W. Piątkowski; I. Poplewska	A case study of the mechanism of unfolding and aggregation of a monoclonal antibody in ion exchange chromatography	2021
19	D. Antos; A. Górak; M. Jaworska	Review on the application of chitin and chitosan in chromatography	2020
20	D. Antos; G. Carta; M. Kołodziej; R. Muca; W. Piątkowski	Effects of negative and positive cooperative adsorption of proteins on hydrophobic interaction chromatography media	2020
21	D. Antos; J. Beck; A. Durauer; R. Hahn; A. Jungbauer; M. Kołodziej; W. Marek; W. Piątkowski; D. Sauer	Scale up of a chromatographic capture step for a clarified bacterial homogenate - Influence of mass transport limitation and competitive adsorption of impurities	2020
22	D. Antos; K. Baran; A. Stańczak	A high-throughput method for fast detecting unfolding of monoclonal antibodies on cation exchange resins	2020
23	D. Antos; P. Antos; M. Balawejder; R. Bochenek; M. Kołodziej; N. Matłok; M. Olbrycht; W. Piątkowski; M. Przywara	Mechanism of nutrition activity of a microgranule fertilizer fortified with proteins	2020
24	D. Antos; K. Baran; W. Marek; W. Piątkowski	Effect of flow behavior in extra-column volumes on the retention pattern of proteins in a small column	2019
25	D. Antos; M. Balawejder; H. Lorenz; M. Olbrycht; W. Piątkowski; I. Poplewska; A. Seidel-Morgenstern	Cooperative Kinetic Model to Describe Crystallization in Solid Solution Forming Systems	2019
26	D. Antos; M. Kołodziej; A. Łyskowski; W. Piątkowski; I. Poplewska; P. Szałański	Determination of protein crystallization kinetics by a through-flow small-angle X-ray scattering method	2019
27	D. Antos; P. Antos; M. Balawejder; R. Bochenek; J. Gorzelany; K. Kania; M. Kołodziej; N. Matłok; M. Olbrycht; W. Piątkowski; M. Przywara; G. Witek	Sposób wytwarzania nawozu wieloskładnikowego o kontrolowanym uwalnianiu składników	2019