The aim of studying and bibliography

The main aim of study: The main aim of the course is theoretical and practical training in the performance and observation cytological preparations using light microscopy, including fluorescence microscopy and methods for labeling and identification of biological material.

The general information about the module: The module includes 15 hours of lectures and 15 hours of laboratories. Laboratories will take place in the OND in Albigowa.

Bibliography required to complete the module

Bibliography used during lectures

1	Litwin J., Gajda M.	Podstawy technik mikroskopowych	Kraków: Wydawnictwo UJ.	2011
2	Tiago Ferreira, Wayne Rasband	ImageJ User Guide	ON-line https://imagej.nih.gov/ij/docs/guide/user-guide.pdf.	2012

Bibliography used during classes/laboratories/others

1

Ewa U. Kurczyńska, Dorota Borowska-Wykręt

Mikroskopia świetlna w badaniach komórki roślinnej : ćwiczenia

Warszawa: Wydawnictwo Naukowe PWN.

2013

Bibliography to self-study

1

Alberto Diaspro ; aut. Roman Amberger [et al.]

Nanoscopy and multidimensional optical fluorescence microscopy

Boca Raton : CRC Press / Taylor & Francis Group.

2010

Basic requirements in category knowledge/skills/social competences

Formal requirements: Registration for the present semester

Basic requirements in category knowledge: Basic knowledge of the principles of the light microscopy and the field of cell biology

Basic requirements in category skills: Computer skills, the ability to self study

Basic requirements in category social competences: Ability of team work

Module outcomes

MEK	The student who completed the module	Types of classes / teaching methods leading to achieving a given outcome of teaching	Methods of verifying every mentioned outcome of teaching	Relationships with KEK	Relationships with PRK
01	has knowledge of the structure and use of different types of microscopy in biological research	lecture	written exam	K_W02+++ K_U07++	P7S_UW P7S_WG
02	can perform the specimen from biological material, to make his observations with using an optical or a fluorescence microscope and analyze the obtained images of the specimen in ImageJ software.	laboratory	test	K_U09+++ K_K02+++	P7S_KR P7S_UW

Attention: Depending on the epidemic situation, verification of the achieved learning outcomes specified in the study program, in particular credits and examinations at the end of specific classes, can be implemented remotely (real-time meetings).

The syllabus of the module

Sem.	TK	The content	realized in	MEK
2	TK01	History of microscopy and types of microscopes	W01, W02	MEK01
2	TK02	Methods of performing and fixation of specimens	W03, W04	MEK01 MEK02
2	TK03	Specimens staining	W05, W06, L01 - L05	MEK01
2	TK04	Staining using antibodies	W07, W08	MEK01
2	TK05	Collection of microscope images	W09, W10, L06-L10	MEK01
2	TK06	Basics of ImageJ software	W11, W12 L11-L15	MEK02
2	TK07	Tracking the drugs in the cell	W13, W14	MEK02
2	TK08	Exam	W15	MEK01 MEK02

The student's effort

The type of classes	The work before classes	The participation in classes	The work after classes
Lecture (sem. 2)	The preparation for a test: 5.00 hours/sem.	contact hours: 15.00 hours/sem.	complementing/reading through notes: 1.00 hours/sem. Studying the recommended bibliography: 3.00 hours/sem.
Laboratory (sem. 2)	The preparation for a Laboratory: 3.00 hours/sem. The preparation for a test: 3.00 hours/sem.	contact hours: 15.00 hours/sem.	Finishing/Making the report: 2.00 hours/sem.
Advice (sem. 2)	The preparation for Advice: 1.00 hours/sem.	The participation in Advice: 1.00 hours/sem.
Credit (sem. 2)	The preparation for a Credit: 5.00 hours/sem.	The written credit: 1.00 hours/sem.

The way of giving the component module grades and the final grade

The type of classes	The way of giving the final grade
Lecture	Written Exam. 51-60% (3); 61-70% (+3); 71-80% (4); 81-90 (+4); 91-100% (5)
Laboratory	Two written tests. 51-60% (3); 61-70% (+3); 71-80% (4); 81-90 (+4); 91-100% (5)
The final grade	The final grade: (K): K = 0,5wL + 0,5wE; where: L, E is possitive grade from laboratory and exam, w - term of test or exam coefficient, w = 1,0 the first term, w = 0,9 the second term, w = 0,8 the third term.

Sample problems

Required during the exam/when receiving the credit
(-)

Realized during classes/laboratories/projects
(-)

Others
(-)

Can a student use any teaching aids during the exam/when receiving the credit : no

The contents of the module are associated with the research profile: yes

1	M. Misiorek; N. Pieńkowska; M. Siorek; Ż. Szymaszek; M. Twardowska; Ł. Uram; S. Wołowiec	Repurposed Drugs Celecoxib and Fmoc-L-Leucine Alone and in Combination as Temozolomide-Resistant Antiglioma Agents—Comparative Studies on Normal and Immortalized Cell Lines, and on C. elegans	2024
2	K. Balawender; K. Bulanda; K. Kroczek; B. Lewandowski; M. Oleksy; S. Orkisz; M. Potoczek; J. Szczygielski; Ł. Uram	Polylactide-based composites with hydroxyapatite used in rapid prototyping technology with potential for medical applications	2023
3	Ż. Szymaszek; M. Twardowska; Ł. Uram; M. Walczak; S. Wołowiec; K. Wróbel	Exploring the Potential of Lapatinib, Fulvestrant, and Paclitaxel Conjugated with Glycidylated PAMAM G4 Dendrimers for Cancer and Parasite Treatment	2023
4	J. Markowicz; W. Rode; Ł. Uram; S. Wołowiec	Synthesis and Properties of α-Mangostin and Vadimezan Conjugates with Glucoheptoamidated and Biotinylated 3rd Generation Poly(amidoamine) Dendrimer, and Conjugation Effect on Their Anticancer and Anti-Nematode Activities	2022
5	J. Markowicz; Ł. Uram; E. Wałajtys-Rode; S. Wołowiec; K. Wróbel	Synthesis of Biotinylated PAMAM G3 Dendrimers Substituted with R-Glycidol and Celecoxib/Simvastatin as Repurposed Drugs and Evaluation of Their Increased Additive Cytotoxicity for Cancer Cell Lines	2022
6	J. Markowicz; W. Rode; Ł. Uram; S. Wołowiec	Biotin Transport-Targeting Polysaccharide-Modified PAMAM G3 Dendrimer as System Delivering α-Mangostin into Cancer Cells and C. elegans Worms	2021
7	M. Dąbrowska; M. Dąbrowski; E. Sikora; Ł. Uram	Antigen presentation capability and AP-1 activation accompany methotrexate-induced colon cancer cell senescence in the context of aberrant β-catenin signaling	2021
8	M. Malinga-Drozd; Ł. Uram; S. Wołowiec; K. Wróbel	Chiral Recognition of Homochiral Poly (amidoamine) Dendrimers Substituted with R- and S-Glycidol by Keratinocyte (HaCaT) and Squamous Carcinoma (SCC-15) Cells In Vitro	2021
9	A. Filipowicz-Rachwał; J. Markowicz; M. Misiorek; Ł. Uram; E. Wałajtys-Rode; S. Wołowiec	Celecoxib substituted biotinylated poly(amidoamine) G3 dendrimer as potential treatment for temozolomide resistant glioma therapy and anti-nematode agent	2020
10	B. Botta; A. Calcaterra; Y. Cau; P. Maj; J. Markowicz; M. Mori; D. Quaglio ; W. Rode; J. Sobich; Ł. Uram; Z. Zieliński	Alvaxanthone, a Thymidylate Synthase Inhibitor with Nematocidal and Tumoricidal Activities	2020
11	B. Król; P. Król; K. Pielichowska; M. Sochacka-Piętal; Ł. Uram; M. Walczak	Synthesis and property of polyurethane elastomer for biomedical applications based on nonaromatic isocyanates, polyesters, and ethylene glycol	2020
12	E. Chmiel; A. Czerniecka-Kubicka; M. Misiorek; M. Pyda; P. Tutka; Ł. Uram; M. Walczak; S. Wołowiec	Stepwise glucoheptoamidation of poly(amidoamine) dendrimer G3 to tune physicochemical properties of the potential drug carrier: in vitro tests for cytisine conjugates	2020
13	J. Czarnik-Kwaśniak; I. Filiks; K. Kwaśniak; M. Stompor; K. Tutaj; Ł. Uram; S. Wołowiec	Glucoheptoamidated polyamidoamine PAMAM G3 dendrimer as a vehicle for succinate linked doxorubicin; enhanced toxicity of DOX against grade IV glioblastoma U-118 MG cells	2020
14	A. Filipowicz-Rachwał; J. Markowicz; M. Misiorek; M. Pichla; Ł. Uram; E. Wałajtys-Rode; S. Wołowiec	The Effect of Biotinylated PAMAM G3 Dendrimers Conjugated with COX-2 Inhibitor (celecoxib) and PPARγ Agonist (Fmoc-L-Leucine) on Human Normal Fibroblasts, Immortalized Keratinocytes and Glioma Cells in Vitro	2019
15	A. Filipowicz-Rachwał; M. Misiorek; Ł. Uram; E. Wałajtys-Rode; A. Winiarz; S. Wołowiec	Synthesis and Different Effects of Biotinylated PAMAM G3 Dendrimer Substituted with Nimesulide in Human Normal Fibroblasts and Squamous Carcinoma Cells	2019
16	A. Filipowicz-Rachwał; M. Szuster; Ł. Uram; E. Wałajtys-Rode; S. Wołowiec	Evaluation of the localization and biological effects of PAMAM G3 dendrimer-biotin/pyridoxal conjugate as HaCaT keratinocyte targeted nanocarrier	2019
17	D. Aebisher; A. Białońska; M. Kopaczyńska; P. Sareło; Ł. Uram; M. Walczak; S. Wołowiec; M. Zaręba	Mixed-Generation PAMAM G3-G0 Megamer as a Drug Delivery System for Nimesulide: Antitumor Activity of the Conjugate Against Human Squamous Carcinoma and Glioblastoma Cells	2019
18	M. Dąbrowska; W. Rode; M. Skoneczny; Ł. Uram	Methotrexate-induced senescence of human colon cancer cells depends on p53 acetylation, but not genomic aberrarions	2019
19	P. Maj; L. Mangiardi; J. Markowicz; W. Rode; J. Sobich; Ł. Uram	Antitumor and anti-nematode activities of α-mangostin	2019