The aim of studying and bibliography

The main aim of study: To provide knowledge on modern methods of chromatographic analysis. Familiarize students with the criteria of selection of suitable chromatographic method, depending on the task of analysis.

The general information about the module:

Teaching materials: Instrukcje do ćwiczeń laboratoryjnych.

Bibliography required to complete the module

Bibliography used during lectures

1	Zygfryd Witkiewicz	Podstawy chromatografii	WNT Warszawa.	2004
2	Zygfryd Witkiewicz, Joanna Kałużna-Czaplińska.	Podstawy chromatografii i technik elektromigracyjnych	WNT, Warszawa.	2014
3	A. Kozik, M. Rąpała-Kozik, I. Guevara-Lora	Analiza instrumentalna w biochemii. Wybrane problemy i metody instrumentalnej biochemii analitycznej	Instytut Biologii Molekularnej UJ, Kraków .	2001
4	W. Szepaniak	Metody instrumentalne w analizie chemicznej	PWN, Warszawa.	1996
5	Marianna Zając, Anna Jelińska	Ocena jakości substancji i produktów leczniczych	UM, Poznań .	2010

Bibliography to self-study

1

Artykuły naukowe z zakresu najnowocześniejszych metod analizy chromatograficznej.

.

Basic requirements in category knowledge/skills/social competences

Formal requirements: Required course credits: physical chemistry, organic chemistry, analytical chemistry, instrumental analysis and instrumental analysis II.

Basic requirements in category knowledge: Knowledge of the basics of chromatographic methods. Knowledge of the sorption / desorption proces. Knowledge of basic physicochemical properties of the chemical compounds.

Basic requirements in category skills: Practical skills in use of basic laboratory glass and measuring equipment. Computer skills in use of word processor and a spreadsheet.

Basic requirements in category social competences: An ability to work in a team.

Module outcomes

MEK	The student who completed the module	Types of classes / teaching methods leading to achieving a given outcome of teaching	Methods of verifying every mentioned outcome of teaching	Relationships with KEK	Relationships with PRK
01	The student has knowledge of the analysis of biotechnological products by clasical chromatographic methods.	lecture	test	K_W05+	P7S_WG
02	The student knows the range of applications of particular techniques of chromatographic analysis of biotechnological products.	lecture	test
03	Utilization of chromatographic methods to conduct the analysis of the composition of the biotech products.	lab classes, lecture	test, observation of performance	K_U11+	P7S_UW
04	Ability to work in a team.	lab classes	observation of performance	K_K02+	P7S_KR

Attention: Depending on the epidemic situation, verification of the achieved learning outcomes specified in the study program, in particular credits and examinations at the end of specific classes, can be implemented remotely (real-time meetings).

The syllabus of the module

Sem.	TK	The content	realized in	MEK
2	TK01	Modern chromatographic methods (GC, GLC, HPLC, GPC) in analysis of biotechnological products. Types of detectors and chromatographic columns and their scope of application in the analysis of biotechnological products. Rules for selection of analysis conditions, depending on the type of sample to be analyzed by gas chromatography and liquid chromatography. Methods for assessing the reliability of results and validation of chromatographic methods. Examples of methods for marking the latest biotechnology products by chromatographic methods.	W01-W14	MEK01 MEK02
2	TK02	The selection and optimization of chromatographic methods for quality and quantity determinations of selected substances in biotechnological products by chromatographic methods such as GC, GC-MS, GC-chiral, GPC, HPLC. Validation of selected parameters of one of the methods used in the lab classes. The preparation of calibration curves, determinations of analytes and analysis of the obtained results.	L01-L20	MEK03 MEK04

The student's effort

The type of classes	The work before classes	The participation in classes	The work after classes
Lecture (sem. 2)		contact hours: 15.00 hours/sem.
Laboratory (sem. 2)	The preparation for a Laboratory: 4.00 hours/sem. The preparation for a test: 4.00 hours/sem.	contact hours: 20.00 hours/sem.	Finishing/Making the report: 4.00 hours/sem.
Advice (sem. 2)		The participation in Advice: 1.00 hours/sem.
Credit (sem. 2)	The preparation for a Credit: 10.00 hours/sem.	The written credit: 1.00 hours/sem.

The way of giving the component module grades and the final grade

The type of classes	The way of giving the final grade
Lecture	test,
Laboratory
The final grade

Sample problems

Required during the exam/when receiving the credit
(-)

Realized during classes/laboratories/projects
(-)

Others
(-)

Can a student use any teaching aids during the exam/when receiving the credit : no

The contents of the module are associated with the research profile: yes

1	K. Bester; A. Bukowska; W. Bukowski; A. Kawka; M. Pytel	Salophen chromium(III) complexes functionalized with pyridinium salts as catalysts for carbon dioxide cycloaddition to epoxides	2024
2	K. Bester; A. Bukowska; W. Bukowski; M. Drajewicz; K. Dychtoń; R. Ostatek; P. Szałański	Sposób wytwarzania salofenowego kompleksu chromu(III)	2024
3	K. Bester; A. Bukowska; W. Bukowski; S. Flaga	Reactive Polymer Composite Microparticles Based on Glycidyl Methacrylate and Magnetite Nanoparticles	2024
4	K. Bester; A. Bukowska; W. Bukowski; M. Drajewicz; K. Dychtoń; R. Ostatek; P. Szałański	Ligand salphenowy oraz sposób syntezy tego ligandu salphenowego	2023
5	K. Bester; W. Bukowski; M. Kaczmarek; D. Tomczyk	Electrocatalytic Properties of Ni(II) Schiff Base Complex Polymer Films	2022
6	K. Bester; W. Bukowski; P. Seliger; D. Tomczyk	The Influence of Electrolyte Type on Kinetics of Redox Processes in the Polymer Films of Ni(II) Salen-Type Complexes	2022
7	K. Bester; A. Bukowska; W. Bukowski	Homogeniczny katalizator chromowy, sposób jego wytwarzania, układ katalityczny zawierający ten katalizator oraz zastosowanie tego układu katalitycznego	2021
8	K. Bester; A. Bukowska; W. Bukowski; M. Pytel	Polymer Beads Decorated with Dendritic Systems as Supports for A3 Coupling Catalysts	2021
9	K. Bester; A. Bukowska; W. Bukowski; M. Pytel; A. Sobota	Copolymerization of Phthalic Anhydride with Epoxides Catalyzed by Amine-Bis(Phenolate) Chromium(III) Complexes	2021
10	K. Bester; A. Bukowska; W. Bukowski	Homogeniczny katalizator chromowy, sposób jego wytwarzania, układ katalityczny zawierający ten katalizator oraz zatosowanie tego układu katalitycznego	2019
11	K. Bester; W. Bukowski; D. Tomczyk	Kinetics of Redox Processes in the Polymer Films of Ni(II) Salen Type Complexes	2019