The aim of studying and bibliography

The main aim of study: To acquaint students with the latest techniques in the field of genetic engineering used in therapy and diagnostics.

The general information about the module: The module is implemented in the second semester, includes 15 hours of lectures and 15 hours laboratory. The module ends with credit.

Bibliography required to complete the module

Bibliography used during lectures

1	Drewa G, Ferenc T.	Genetyka medyczna. Podręcznik dla studentów	Urban & Partner.	2011
2	Bal J.	Biologia molekularna w medycynie	PWN, Warszawa.	2008
3	Addgene	CRISPR 101: A desktop resurce	Addgene.	2016
4	źródłowe prace przeglądowe	Biotechniques, Nature Methods,Current oppinion ..., Trends in ...	.
5	Primrose S.B., Twyman R.M., Old R.W.	Principles of Gene Manipulation	Wiley-Blackwell.	2006

Bibliography used during classes/laboratories/others

1

Instrukcje do ćwiczeń

.

Bibliography to self-study

1

Bamshad MJ, Carey C.J, Jorde L.B.

Genetyka medyczna

Urban & Partner,.

2002

Basic requirements in category knowledge/skills/social competences

Formal requirements: Knowledge of genetic basics , molecular biology, genetic engineering and bioinformatics

Basic requirements in category knowledge: Has specific knowledge about genetic engineering methods and the ability to use them to identify the factors that cause diseases

Basic requirements in category skills: Student mastered the use of the automatic pipette and knows the basic rules of behavior in the laboratory

Basic requirements in category social competences: Ability to cooperate as a member of team, is ethically prepared to work in field of biotechnology

Module outcomes

MEK	The student who completed the module	Types of classes / teaching methods leading to achieving a given outcome of teaching	Methods of verifying every mentioned outcome of teaching	Relationships with KEK	Relationships with PRK
01	Knows the latest genetic engineering techniques used to edit the genome. Understands the importance of these methods in order to adapt organisms as bio-indicators and conveyors of drugs in targeted therapy.	lecture	written exam	K_W06+++	P7S_WG
02	Able to identify the defective gene changes and suggest correction. Knows the defects and benefits of the most important systems used to deliver genetic material and can adapt to the needs of the system..	laboratory	project report, written exam	K_K02++	P7S_KR

Attention: Depending on the epidemic situation, verification of the achieved learning outcomes specified in the study program, in particular credits and examinations at the end of specific classes, can be implemented remotely (real-time meetings).

The syllabus of the module

Sem.	TK	The content	realized in	MEK
2	TK01	Introduction to the genomic engineering. Characteristic of CRISPR method and its research application. CRISPR expression method , therapeutic applications. Genetic modification of organisms for the purposes of use in targeted therapy and diagnosis.	w1	MEK01
2	TK02	The identification of defective mutations with the selected genes.Design of gene structure adapted to different systems used in gene therapy, using Gateway technology, silencing RNAi and CRISPR. Designing genetic modifications aimed at creating a bio-indicators.	L01-L03	MEK02

The student's effort

The type of classes	The work before classes	The participation in classes	The work after classes
Lecture (sem. 2)		contact hours: 15.00 hours/sem.
Project/Seminar (sem. 2)	The preparation for projects/seminars: 5.00 hours/sem.	contact hours: 15.00 hours/sem..	Doing the project/report/ Keeping records: 10.00 hours/sem.
Advice (sem. 2)		The participation in Advice: 2.00 hours/sem.
Credit (sem. 2)	The preparation for a Credit: 8.00 hours/sem.	The written credit: 2.00 hours/sem.

The way of giving the component module grades and the final grade

The type of classes	The way of giving the final grade
Lecture
Project/Seminar
The final grade

Sample problems

Required during the exam/when receiving the credit
(-)

Realized during classes/laboratories/projects
(-)

Others
(-)

Can a student use any teaching aids during the exam/when receiving the credit : no

The contents of the module are associated with the research profile: yes

1	B. Bakera; M. Rakoczy-Trojanowska; M. Szeliga; M. Święcicka; M. Tyrka	Identification of candidate genes responsible for chasmogamy in wheat	2023
2	P. Bednarek; A. Dorczyk; T. Drzazga; D. Jasińska; P. Krajewski; B. Ługowska; R. Martofel; P. Matysik; M. Niewińska; D. Ratajczak; K. Rączka; T. Sikora; D. Tyrka; M. Tyrka; E. Witkowski; U. Woźna-Pawlak	Genome-wide association mapping in elite winter wheat breeding for yield improvement	2023
3	M. Dyda; G. Gołębiowska; M. Rapacz; M. Szechyńska-Hebda; M. Tyrka; I. Wąsek; M. Wędzony	Quantitative trait loci and candidate genes associated with freezing tolerance of winter triticale (× Triticosecale Wittmack)	2022
4	M. Dyda; G. Gołębiowska; M. Rapacz; M. Tyrka; M. Wędzony	Genetic mapping of adult-plant resistance genes to powdery mildew in triticale	2022
5	M. Dyda; G. Gołębiowska; M. Rapacz; M. Tyrka; M. Wędzony	Mapping of QTL and candidate genes associated with powdery mildew resistance in triticale (× Triticosecale Wittm.)	2022
6	P. Krajewski; R. Marcinkowski; R. Martofel; P. Matysik; M. Mokrzycka; M. Rakoczy-Trojanowska; M. Rokicki; S. Stojałowski; M. Tyrka; U. Woźna-Pawlak; B. Żmijewska	Genome-Wide Association Analysis for Hybrid Breeding in Wheat	2022
7	A. Pietrusińska; M. Tyrka	Linkage of Lr55 wheat leaf rust resistance gene with microsatellite and DArT-based markers	2021
8	B. Bakera; P. Krajewski; M. Mokrzycka; M. Rakoczy-Trojanowska; M. Szeliga; M. Święcicka; M. Tyrka	Identification of Rf Genes in Hexaploid Wheat (Triticumaestivum L.) by RNA-Seq and Paralog Analyses	2021
9	B. Bakera; P. Krajewski; P. Matysik; M. Mokrzycka; M. Rakoczy-Trojanowska; M. Rokicki; S. Stojałowski; M. Szeliga; D. Tyrka; M. Tyrka	Evaluation of genetic structure in European wheat cultivars and advanced breeding lines using high-density genotyping-by-sequencing approach	2021
10	J. Buczkowicz; T. Drzazga; B. Ługowska; P. Matysik; K. Rubrycki; M. Semik; D. Tyrka; M. Tyrka; E. Witkowski	Identyfikacja efektywnych genów odporności na wybrane choroby wirusowe i grzybowe pszenicy zwyczajnej	2021
11	J. Buczkowicz; T. Drzazga; G. Fic; M. Jaromin; P. Krajewski; P. Matysik; R. Mazur; P. Milczarski; T. Sikora; M. Szeliga; D. Tyrka; M. Tyrka; E. Witkowski	Selekcja genomowa pszenicy ozimej	2021
12	E. Ciszkowicz; E. Kaznowska; P. Porzycki; M. Semik; M. Tyrka	MiR-93/miR-375: Diagnostic Potential, Aggressiveness Correlation and Common Target Genes in Prostate Cancer	2020
13	G. Czajowski; M. Karbarz; M. Pojmaj; A. Strzembicka; D. Tyrka; M. Tyrka; A. Wardyńska; M. Wędzony	Quantitative trait loci mapping of adult-plant resistance to powdery mildew in triticale	2020
14	J. Ciura; M. Szeliga; M. Tyrka	Representational Difference Analysis of Transcripts Involved in Jervine Biosynthesis	2020
15	J. Ciura; M. Grzesik; M. Szeliga; M. Tyrka	Identification of candidate genes involved in steroidal alkaloids biosynthesis in organ-specific transcriptomes of Veratrum nigrum L.	2019
16	M. Dyda; M. Szechyńska-Hebda; M. Tyrka; I. Wąsek; M. Wędzony	Local and systemic regulation of PSII efficiency in triticale infected by the hemibiotrophic pathogen Microdochium nivale	2019
17	M. Dziurka; K. Hura; T. Hura; A. Ostrowska; M. Tyrka	Participation of Wheat and Rye Genome in Drought Induced Senescence in Winter Triticale (X Triticosecale Wittm.)	2019
18	Z. Banaszak; A. Fiust; Z. Nita; W. Orłowska-Job; M. Pojmaj; M. Rapacz; M. Tyrka; M. Wójcik-Jagła	Sposób selekcji mrozoodpornych genotypów jęczmienia ozimego	2019