The aim of studying and bibliography

The main aim of study: Familiarize students with basic concepts of classical and molecular cytogenetics, as well as the latest issues applied in laboratory diagnostics.

The general information about the module: The module is introduced in the 2nd semester of the second degree/ graduate course as a faculty module for specialty. It is accomplished with 15 hours of lecture and 20 hours of laboratory work. Lectures are conducted in the form of Power Point and Prezi presentations.

Bibliography required to complete the module

Bibliography used during lectures

1

Rogalska S., Małuszyńska J., Olszewska M.J.

Podstawy cytogenetyki roślin

Wydawnictwo Naukowe PWN, Warszawa .

2012

Basic requirements in category knowledge/skills/social competences

Formal requirements: Valid registration

Basic requirements in category knowledge: Basic knowledge of nucleic acid and chromosome structure.

Basic requirements in category skills: Self-education skills

Basic requirements in category social competences: Ability to work both alone and in a group.

Module outcomes

MEK	The student who completed the module	Types of classes / teaching methods leading to achieving a given outcome of teaching	Methods of verifying every mentioned outcome of teaching	Relationships with KEK	Relationships with PRK
01	Knows the structure of chromosomes in different organisms.	lecture	written test	K_W06+	P7S_WG
02	Is able to conduct experiments using the nucleic acid using the methods of molecular biology.	laboratory excercises	test, written raport, observation of performance	K_K02+++	P7S_KR
03	Extended knowledge of preparation of plant and animal cells for cytogenetic analysis.	lecture, laboratory	written test, raport, observation	K_W03+++	P7S_WG

Attention: Depending on the epidemic situation, verification of the achieved learning outcomes specified in the study program, in particular credits and examinations at the end of specific classes, can be implemented remotely (real-time meetings).

The syllabus of the module

Sem.	TK	The content	realized in	MEK
2	TK01	-	W01-W03	MEK01
2	TK02	In vivo, in vitro and in situ in cytogenetic studies. Cytogenetic effects of DNA damage. Apoptosis and necrosis.	W04-W08	MEK01
2	TK03	Cytogenetic methods of analysis. Fluorescence in situ hybridization, its development and modifications.	W09-W12	MEK01 MEK03
2	TK04	Modern methods of cytogenetic diagnosis in medicine.	W13-W15	MEK03
2	TK05	Preparation of material for cytogenetic studies.	L01	MEK02
2	TK06	Karyotype analysis of plant and/or animal cells.	L02	MEK02
2	TK07	Fluorescence in situ hybridization.	L03	MEK02
2	TK08	Comet assay.	L04	MEK02

The student's effort

The type of classes	The work before classes	The participation in classes	The work after classes
Lecture (sem. 2)		contact hours: 15.00 hours/sem.
Laboratory (sem. 2)		contact hours: 20.00 hours/sem.	Finishing/Making the report: 4.00 hours/sem.
Advice (sem. 2)		The participation in Advice: 2.00 hours/sem.
Credit (sem. 2)	The preparation for a Credit: 15.00 hours/sem.	The written credit: 2.00 hours/sem.

The way of giving the component module grades and the final grade

The type of classes	The way of giving the final grade
Lecture	The grade is issued in a manner appropriate to the number of points received during the final tes: 50-59% - [3], 60-69% - [3,5], 70-79% - [4], 80-89 - [4,5], 90-100 - [5]
Laboratory	The grade is issued on the basis of the average grade of the tests. Test points: 50-59% - [3], 60-69% - [3,5], 70-79% - [4], 80-89 - [4,5, 90-100 - [5].
The final grade	The final grade is the average of the grade of lectures and a final evaluation labs K=0,4wL+0,1wS+0,5wZ (L-llaboratory, Z-final test, S-raport, w-coefficient taking into account term, w = 1 for the first term. W = 0.9 for the second term in = 0.8 for the third term). The average may be drawn only if all grades are positive

Sample problems

Required during the exam/when receiving the credit
(-)

Realized during classes/laboratories/projects
(-)

Others
(-)

Can a student use any teaching aids during the exam/when receiving the credit : no

The contents of the module are associated with the research profile: yes

1	E. Ciszkowicz; M. Dżugan; K. Lecka-Szlachta; M. Miłek; M. Tomczyk	Coniferous Honeydew Honey: Antibacterial Activity and Anti-Migration Properties against Breast Cancer Cell Line (MCF-7)	2024
2	K. Awsiuk; J. Bała; P. Chmielarz; E. Ciszkowicz; J. Raczkowska; M. Sroka; K. Wolski; I. Zaborniak	Grafting of Multifunctional Polymer Brushes from a Glass Surface: Surface-Initiated Atom Transfer Radical Polymerization as a Versatile Tool for Biomedical Materials Engineering	2024
3	Ł. Byczyński; E. Ciszkowicz; D. Czachor-Jadacka; M. Kisiel; B. Mossety-Leszczak; B. Pilch-Pitera; M. Walczak; J. Wojturska	Wodna dyspersja kationomerów uretanowo-akrylowych, sposób wytwarzania wodnej dyspersji kationomerów uretanowo-akrylowych oraz sposób wytwarzania fotoutwardzalnej powłoki z wykorzystaniem tej wodnej dyspersji	2024
4	E. Ciszkowicz; M. Dżugan; J. Hęclik; K. Lecka-Szlachta; M. Miłek; E. Sidor	The Antioxidant, Antibacterial and Anti-Biofilm Properties of Rapeseed Creamed Honey Enriched with Selected Plant Superfoods	2023
5	E. Ciszkowicz; B. Creaven; H. Jenkins; D. Kamiński; D. Karcz; K. Lecka-Szlachta; A. Matwijczuk; A. Miłoś; K. Starzak; L. Ślusarczyk	Design, Spectroscopy, and Assessment of Cholinesterase Inhibition and Antimicrobial Activities of Novel Coumarin–Thiadiazole Hybrids	2022
6	E. Ciszkowicz; M. Dżugan; K. Lecka-Szlachta; M. Miłek; A. Miłoś; A. Pasternakiewicz ; G. Zaguła	Mineral Composition, Antioxidant, Anti-Urease, and Antibiofilm Potential of Juglans Regia Leaves and Unripe Fruits	2022
7	E. Ciszkowicz; M. Dżugan; K. Lecka-Szlachta; M. Miłek; A. Pasternakiewicz ; E. Sidor ; M. Tomczyk; G. Zaguła	The Study of Chemical Profile and Antioxidant Properties of Poplar-Type Polish Propolis Considering Local Flora Diversity in Relation to Antibacterial and Anticancer Activities in Human Breast Cancer Cells	2022
8	E. Ciszkowicz; M. Kosińska-Pezda; K. Lecka-Szlachta; A. Miłoś; E. Woźnicka; L. Zapała	Analiza właściwości antybakteryjnych oraz cytotoksyczności kompleksów jonów Co(II), Mn(II) oraz Zn(II) z chryzyną oraz 3-hydroksyflawonem	2022
9	P. Chmielarz; E. Ciszkowicz; K. Lecka-Szlachta; A. Macior; J. Smenda; K. Wolski; I. Zaborniak	A New Protocol for Ash Wood Modification: Synthesis of Hydrophobic and Antibacterial Brushes from the Wood Surface	2022
10	E. Ciszkowicz; B. Creaven; H. Jenkins; D. Kamiński; D. Karcz; K. Lecka-Szlachta; A. Matwijczuk; A. Miłoś; P. Radomski; K. Starzak; L. Ślusarczyk	Novel Coumarin-Thiadiazole Hybrids and Their Cu(II) and Zn(II) Complexes as Potential Antimicrobial Agents and Acetylcholinesterase Inhibitors	2021
11	E. Ciszkowicz; K. Lecka-Szlachta; J. Pusz; E. Woźnicka	Antybakteryjna aktywność sulfonowych pochodnych chryzyny	2021
12	E. Ciszkowicz; M. Kosińska-Pezda; A. Kuźniar; K. Lecka-Szlachta; E. Pieniążek; E. Woźnicka; L. Zapała	Synteza, charakterystyka i właściwości biologiczne kompleksów jonów antanowców lekkich z 3-hydroksyflawonem	2021
13	Ł. Byczyński; E. Ciszkowicz; M. Kosińska-Pezda; K. Lecka-Szlachta; U. Maciołek; E. Woźnicka; L. Zapała; W. Zapała	Green synthesis of niflumic acid complexes with some transition metal ions (Mn(II), Fe(III), Co(II), Ni(II), Cu(II) and Zn(II)). Spectroscopic, thermoanalytical and antibacterial studies	2021
14	A. Bocian; J. Buczkowicz; E. Ciszkowicz; K. Hus; K. Lecka-Szlachta; J. Legath; L. Legath; V. Petrilla; M. Petrillova; M. Pietrowska	Antimicrobial Activity of Protein Fraction from Naja ashei Venom Against Staphylococcus epidermidis	2020
15	E. Ciszkowicz; B. Creaven; D. Kamiński; D. Karcz; K. Lecka-Szlachta; A. Matwijczuk; K. Starzak	Structural Features of 1,3,4-Thiadiazole-Derived Ligands and Their Zn(II) and Cu(II) Complexes Which Demonstrate Synergistic Antibacterial Effects with Kanamycin	2020
16	E. Ciszkowicz; E. Kaznowska; P. Porzycki; M. Semik; M. Tyrka	MiR-93/miR-375: Diagnostic Potential, Aggressiveness Correlation and Common Target Genes in Prostate Cancer	2020
17	E. Ciszkowicz; P. Porzycki	Modern biomarkers in prostate cancer diagnosis	2020
18	E. Ciszkowicz; P. Porzycki	Detection of individual prostate cancer via multiparametric magnetic resonance imaging in own material - initial experience	2019
19	Ł. Byczyński; M. Chutkowski; E. Ciszkowicz; M. Kosińska; K. Lecka-Szlachta; E. Woźnicka; L. Zapała; W. Zapała	Comparison of spectral and thermal properties and antibacterial activity of new binary and ternary complexes of Sm(III), Eu(III) and Gd (III) ions with N-phenylanthranilic acid and 1,10-phenanthroline	2019